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Re: Ask Quick Questions, Get Quick Answers [Re: san pedro guy] * 1
    #29130461 -

san pedro guy said:
I’m going to test if an old dried up plate will grow again (don’t think it will) and I’m wondering the best option in yall opinion…..

1. try to crack a piece off onto a fresh plate
2. drop a large piece of wet agar on top of the dry stuff
3. squirt sterile water on the dry plate and then do a transfer after it’s rehydrated
4. something I’m not thinking of?

tia


You could pour warm agar over entire plate.


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Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
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Re: Ask Quick Questions, Get Quick Answers [Re: tripground]
    #29130462 -

tripground said:
Hi everyone, why my cake has this gap? I think it prevents mycellium to grow to bottom. How can I fix this? I have 13 jars and almost all of them looks like this, and I'm waiting for 1.5 months. It's a lot. Temperature is good btw.





Wait, all 13 jars have been growing for 1.5 months and have a crack like that from which they won't grow further?

Im going to guess something is going very wrong but as to what that is, i couldn't tell ya.


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Endlessly endorsing empathy empirically energizing existence™

~Always practice radical kindness~
R.I.P. Betty Boop, greatest mother to ever do it.

Edited by QuantumZ00 (02/18/25 03:40 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: QuantumZ00]
    #29130469 -

Yes. A few jars colonized %90 but they have a little gap in the bottom. And other jars looks like %50, like the one I posted.

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Re: Ask Quick Questions, Get Quick Answers [Re: ReverendMyc] * 1
    #29130476 -

ReverendMyc said:
https://www.amazon.com/XPRS-Nutra-Vegan-Separated-Capsules/dp/B0BHTV7MB3
I love these vegan ones. I'm not vegan, but they are said to be easier and quicker to digest than gelatin. I usually do 00 and sort my own, but I still feel good about recommending these.


The two color ones would probably be easy to sort to save some $$$. 10x for a couple dollars more? I'll sort, thanks. :wink::moneyeyes::money:
https://www.amazon.com/dp/B088D394S9


Yeah, good point. Thanks brotha!


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #29130575 -

Out of curiosity has anyone, or is it possible; to put a piece of years old dried mushroom onto agar to grow it out?

I have some stuff that I never stored genetics from I was thinking about reviving.

If you were to break a part off and place a flake from the inside of the stipe would that grow? Or is this not a feasible option.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Ask Quick Questions, Get Quick Answers [Re: potato11teen]
    #29130578 -

potato11teen said:
Umm, I just got back from a weekend vacation. What the fuck did I grow.

Looks like low fresh air. Why didn't the caps not develop? Senescence? I did grow this first batch from an LC from a vendor.

Species is Ochraceocentrata. Whatever these are, they're blue on the inside.





Were these grown in darkness?


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𝕴𝖋 𝕴 𝖍𝖆𝖛𝖊 𝖘𝖊𝖊𝖓 𝖋𝖚𝖗𝖙𝖍𝖊𝖗 𝖎𝖙 𝖎𝖘 𝖇𝖞 𝖘𝖙𝖆𝖓𝖉𝖎𝖓𝖌 𝖚𝖕𝖔𝖓 𝖙𝖍𝖊 𝖘𝖍𝖔𝖚𝖑𝖉𝖊𝖗𝖘 𝖔𝖋 𝕲𝖎𝖆𝖓𝖙𝖘

:drugnerd: The TekDek v.2    Autist Tally :laugh:

:mushroom2:How to make a TEK:mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: Tri]
    #29130582 -

Question of field capacity for JUST pure coir:

Without verm, what should I be looking for. I've heard different ratios from different posters across the years. Normally I'm 5xing my dry coir weight with water. So, what is your field capacity?

Squeeze and quick stream then nothing?

or

Squeeze hard, few droplets, then nothing?

I normally go for the second, and I'm wondering if my substrate might be too dry.


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I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies] * 2
    #29130587 -

Huskies said:
Question of field capacity for JUST pure coir:

Without verm, what should I be looking for. I've heard different ratios from different posters across the years. Normally I'm 5xing my dry coir weight with water. So, what is your field capacity?

Squeeze and quick stream then nothing?

or

Squeeze hard, few droplets, then nothing?

I normally go for the second, and I'm wondering if my substrate might be too dry.


Few drops then nothing, then I do a heavy mist on a top pseudo casing in order to promote pinning on the surface, so its hydrated less in the main substrate but more hydrated on the pseudo casing on the top.

I very rarely have bad outbreaks of side pins, just an occasional here and there and I don't use liners.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Ask Quick Questions, Get Quick Answers [Re: Axman]
    #29130595 -

OOh I've been getting a lot of side pins, but I like that idea of misting it. You do the misting at spawn?


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies] * 2
    #29130605 -

Huskies said:
OOh I've been getting a lot of side pins, but I like that idea of misting it. You do the misting at spawn?


I mix my sub and myco quart at the above mentioned field capacity, add at least a 1/2" of a pseudo casing at the same field capacity, then mist the heck out of it.

If I'm spawning a shoebox, I'll mist while counting to 20. It will be quite wet, but it seems to set my surface conditions well.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Ask Quick Questions, Get Quick Answers [Re: Axman] * 2
    #29130620 -

Axman said:
Out of curiosity has anyone, or is it possible; to put a piece of years old dried mushroom onto agar to grow it out?

I have some stuff that I never stored genetics from I was thinking about reviving.

If you were to break a part off and place a flake from the inside of the stipe would that grow? Or is this not a feasible option.


I've heard of it being done; I'm pretty sure it wasn't a mycelial clone,rather, a spore collection from the gills. If I remember right, extreme measures were needed to force hydrate the collected spores...

I say try and John Hammond those genetics!


--------------------
Check out my wish list and let me know what you got! UPDATED: 2/22/25
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Re: Ask Quick Questions, Get Quick Answers [Re: mike-ologist] * 1
    #29130641 -

mike-ologist said:
I've heard of it being done; I'm pretty sure it wasn't a mycelial clone,rather, a spore collection from the gills. If I remember right, extreme measures were needed to force hydrate the collected spores...

I say try and John Hammond those genetics!


I'm considering trying to put some of the dried stipe into some sterile distilled h20 and putting it on agar and brf pucks.

I was curious if anyone has gone down this rabbit hole before. It's a variety of Gandolf that I'm trying to reclaim, and I'm not sure if it sporulates or not.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Ask Quick Questions, Get Quick Answers [Re: mike-ologist]
    #29130644 -

Trying to clean up a dirty GT print. The germ plate didn't look great. I came across the trenching technique, giving it a try.

Does this look right?

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Re: Ask Quick Questions, Get Quick Answers [Re: Hotboxer]
    #29130660 -

Mycelium or mould I've noticed a bit of fuzz on these cordyceps that weren't around a few days ago hard to tell. It also grew this much very fast within 3 days then slowed down and changed colour a lil to a more orange it came from liquid culture and has been around 2 weeks since squirtee


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off on an adventure, exploring entheogens🌱🌌

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Re: Ask Quick Questions, Get Quick Answers [Re: tripground] * 1
    #29130669 -

tripground said:
Hi everyone, why my cake has this gap? I think it prevents mycellium to grow to bottom. How can I fix this? I have 13 jars and almost all of them looks like this, and I'm waiting for 1.5 months. It's a lot. Temperature is good btw.




Looks like you’re suffocating it. No gas exchange will cause the stuff on the top to colonize leaving the stuff on the bottom to do nothing. I would take the colonized piece out. Remove all uncolonized grain and fruit it. 

In the future you need smaller jars for PF tek.


--------------------
The Hitchikers Guide to the Shroomery

Sometimes you just have to shut up and color!
~CMS Wilbur

Edited by OLDDAD77 (02/18/25 07:16 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Hotboxer]
    #29130670 -

Hotboxer said:
Trying to clean up a dirty GT print. The germ plate didn't look great. I came across the trenching technique, giving it a try.

Does this look right?



Just read up on that yesterday, but yeah seems good! curious to see that jump the gap


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Axman] * 2
    #29130687 -

Axman said:
Out of curiosity has anyone, or is it possible; to put a piece of years old dried mushroom onto agar to grow it out?

I have some stuff that I never stored genetics from I was thinking about reviving.

If you were to break a part off and place a flake from the inside of the stipe would that grow? Or is this not a feasible option.


Ppl have definitely tried and succeeded.  More trying than succeeding tho.  The barriers are if it was dried using too much heat and the insane amount of contamination now on the sample.  Dried tissue is 100% revivable.  Whether or not that tissue is dead or beyond getting clean is the issue.

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Re: Ask Quick Questions, Get Quick Answers [Re: fahtster]
    #29130692 -

Quote:
fahtster

Ppl have definitely tried and succeeded.  More trying than succeeding tho.  The barriers are if it was dried using too much heat and the insane amount of contamination now on the sample.  Dried tissue is 100% revivable.  Whether or not that tissue is dead or beyond getting clean is the issue.


I appreciate the input, I think I'll go ahead and give it a whirl.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Ask Quick Questions, Get Quick Answers [Re: Axman] * 4
    #29130702 -

Im almost certain that gill fragments reactivated when trying to revive old spore stock, here:

https://www.shroomery.org/forums/showflat.php/Number/28790126#28790126

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Re: Ask Quick Questions, Get Quick Answers [Re: Stipe-n Cap] * 5
    #29130719 -

I think this plate's ok? Not 100%



--------------------
Learn it, know it, live it


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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