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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Camera93] * 2
    #29129764 -

Camera93 said:
Sorta like "ass to mouth", in the heat of the moment does anyone go clone tissue plate -> to grain?

Assuming no satellites or bacterial blooms present on the plate?

I haven't done it, but sorta thinking why not if the tissue sample was sterile and taken to plate in flow

:takingnotes:


I always go clone plate to grain.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 1
    #29129766 -

I should've asked sooner...
:cheers:


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.
Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can

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Re: Ask Quick Questions, Get Quick Answers [Re: Camera93] * 2
    #29129767 -

Not ashamed to say: I do tissue to grain pint master in the sab
Kinda josex poke but trying to aim for chunky filaments.


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“All this buttoning and unbuttoning”

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Re: Ask Quick Questions, Get Quick Answers [Re: 999999999] * 2
    #29129775 -

Tissue to grain, plin to grain, clone plate to grain. It’s all Gucci. I try to put as few steps between the genes i want and the genes im running. Barring a mishap, indoor mushrooms always take clean clones.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 1
    #29129864 -

Umm, I just got back from a weekend vacation. What the fuck did I grow.

Looks like low fresh air. Why didn't the caps not develop? Senescence? I did grow this first batch from an LC from a vendor.

Species is Ochraceocentrata. Whatever these are, they're blue on the inside.




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Accept the pain, but don't accept that you deserved it.

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #29129890 -

Smellyhobbit said:
Tissue to grain, plin to grain, clone plate to grain. It’s all Gucci. I try to put as few steps between the genes i want and the genes im running. Barring a mishap, indoor mushrooms always take clean clones.


What about when you want to expand that tissue plate? Then you are still introducing steps, something you keep in mind there or transfers as usual?

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Re: Ask Quick Questions, Get Quick Answers [Re: eilauver] * 4
    #29129895 -

eilauver said:
Smellyhobbit said:
Tissue to grain, plin to grain, clone plate to grain. It’s all Gucci. I try to put as few steps between the genes i want and the genes im running. Barring a mishap, indoor mushrooms always take clean clones.


What about when you want to expand that tissue plate? Then you are still introducing steps, something you keep in mind there or transfers as usual?


When I want to expand the tissue plate I do it, but I like to use as much of the original as possible. Usually the subsequent Ts are totally fine. Like identical, basically.

Just understand now before you get too far into things that no culture lasts forever. Not really. It changes over time and eventually they get lost. Best thing to do is get good at making good cultures and let go of the idea that you’ll ever truly keep them. These are living, changing, thinking things. They don’t like staying the same forever. That’s why we find so much variety in just cubes.

Enjoy the process because it’s all you’ve got


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 2
    #29130007 -

:whathesaid:


Plus you can always expand with g2g and g2stasis

Edited by LewDoja (02/18/25 03:41 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: zbarron]
    #29130014 -

GenesisCorrupted said:
Can you get closer?
I just wanna see the white part of the bag.


zbarron said:


Does anyone know what’s with this weird white film at the top of these grow bags? Prior to inoculation they looked like condensation.


Maybe reach out to the vendor and ask them? If it's anything unwanted they should replace it.


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29130018 -



One all white natty and my jar natty. Cloned the all white one, is it possible it's luci or albino?


--------------------
Check out my wish list and let me know what you got! UPDATED: 2/22/25
Current wish list


Edited by mike-ologist (02/18/25 04:06 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: mike-ologist]
    #29130152 -

I’m going to test if an old dried up plate will grow again (don’t think it will) and I’m wondering the best option in yall opinion…..

1. try to crack a piece off onto a fresh plate
2. drop a large piece of wet agar on top of the dry stuff
3. squirt sterile water on the dry plate and then do a transfer after it’s rehydrated
4. something I’m not thinking of?

tia

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Re: Ask Quick Questions, Get Quick Answers [Re: san pedro guy]
    #29130155 -

If it can be revived, 1 or 2 sounds great. People have definitely revived dried plates before. Good luck!


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 2
    #29130220 -

Finishe picking these side pins and turned em into tee:





Put everything back in its tub.

Should I go ahead and water the tub in the sink in hopes of getting another flush?

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Re: Ask Quick Questions, Get Quick Answers [Re: Peleus] * 1
    #29130226 -

I have this in a shoebox of sorts. As you can see, its got a great microclimate and its pinning like mad.



Thing is, it doesn't have a lot of space for growth, and  this is a clone of a 100g monster



Should I just leave it as is until they are touching the lid? Or do I try putting it in a bigger tub?

Also, these are experiments with chickpea flour, but as you can see, they did not colonize the brick at the bottom. But they are pinning like mad. Think I should just spawn these as is ? I don't want to squish these pins that look so happy.







--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: san pedro guy] * 2
    #29130265 -

san pedro guy said:
I’m going to test if an old dried up plate will grow again (don’t think it will) and I’m wondering the best option in yall opinion…..

1. try to crack a piece off onto a fresh plate
2. drop a large piece of wet agar on top of the dry stuff
3. squirt sterile water on the dry plate and then do a transfer after it’s rehydrated
4. something I’m not thinking of?

tia


What Smelly said. Also brf pucks are great for recovering at risk cultures.


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Ask Quick Questions, Get Quick Answers [Re: ReverendMyc] * 3
    #29130327 -

Thank you both,

I’ll post if it’s a success :thumbup:

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Re: Ask Quick Questions, Get Quick Answers [Re: san pedro guy]
    #29130416 -

Anybody have a recommendation for presorted 000 capsules? The ones on amazon have bad reviews.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 2
    #29130420 -

My currents are OO capsuline from Amazon. I have no complaints. Not sure brands I’ve used in the past but never had issues with any of them.

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Re: Ask Quick Questions, Get Quick Answers [Re: PancyanterA] * 1
    #29130429 -

https://www.amazon.com/XPRS-Nutra-Vegan-Separated-Capsules/dp/B0BHTV7MB3
I love these vegan ones. I'm not vegan, but they are said to be easier and quicker to digest than gelatin. I usually do 00 and sort my own, but I still feel good about recommending these.


The two color ones would probably be easy to sort to save some $$$. 10x for a couple dollars more? I'll sort, thanks. :wink::moneyeyes::money:
https://www.amazon.com/dp/B088D394S9


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

Edited by ReverendMyc (02/18/25 03:22 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: ReverendMyc]
    #29130457 -

Hi everyone, why my cake has this gap? I think it prevents mycellium to grow to bottom. How can I fix this? I have 13 jars and almost all of them looks like this, and I'm waiting for 1.5 months. It's a lot. Temperature is good btw.


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Shop: Myyco.com APE Liquid Culture For Sale   North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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