Axman said:

2-1-25:
I decided to attempt a cross between AA+ and a Tico F-1 Fruit.
I took a swab, swabbed the AA+, then Tico, then AA+, and then back to Tico
Did that for two different fruits of each and put them to LME agar.

---

I've never tried anything like this before so here's to new things.

bluespirit13 said:

I’m in - this will be a great way to kick off my agar journey I suppose. Will probably do Jack Frost.

EDIT - will be doing Oak Ridge since a kind member on here sent me a print so I don’t have to order spores.

12/28 - my first agar! I couldn’t find glad mini rounds anywhere to do pasty plates (have some in the mail but won’t be here until 1/4) so I used some 4oz jars with a filter patch.



1/1 - 8 plates streaked and last one got the broken off swab tip. The larger containers ended up having a lot of moisture so I don’t have high hopes for those ones

This is my first time using agar and I’m super excited!






1/7 - growth spotted on 3 out of 9 plates 👍 good growth? Bad growth? Who knows! But we about to find out lol.

1/10 - making some plates for transfers - will do that tonight or tomorrow. How’s the growth looking so far? First time using agar so I’m not sure. Pics suck because the containers are far from ideal.




1/11 - made first transfers. My pasty plates in the glad mini rounds turned out far from ideal. Some agar was soupy (dunno why) I ended up doing 4 agar plates and then making 2 transfers to BRF pucks which are my kind of “fail safe” if the pasty plates fail.

I kept 3 plates from the initial streak around that look the most clean just in case I need to transfer from them again.


1/14 - made some more agar up for next transfers - did a Josex Poke from an initial streak plate and sent it to a BRF puck just for fun. We will see how it goes. Everything looking clean so hopefully next transfers will be ready to go to grain!

1/18 - some grain prepped - my first time in like 14 years - also a photo of brf puck from an agar transfer looking great





2/2 updates - I have one quart of WBS that I dropped some wedges in on 1/19 - looking really good so far - I also have another very clean looking plate now thanks to a poke from a brf puck. Planning on dropping that to grain this week. Things are looking good 👍

---

Axman said:

2-4-25:
-{Tico Update}-

The tub spawned on 1-30 is coming along nicely.

-{Amazonian Update}-

Hydrating the swabs was definitely the move.


I'll be taking transfers from that plate soon.

I threw some sketchy transfers to grain while a little tipsy the other night.

-{Tico x AA+ Update}-
Three out of the four plates are showing signs of germination.

---

Axman said:

Axman said:

2-4-25:
-{Tico Update}-

The tub spawned on 1-30 is coming along nicely.

-{Amazonian Update}-

Hydrating the swabs was definitely the move.


I'll be taking transfers from that plate soon.

I threw some sketchy transfers to grain while a little tipsy the other night.

-{Tico x AA+ Update}-
Three out of the four plates are showing signs of germination.

---

So far I have 2 tubs of Tico spawned. Hoping within a few more transfers I can have some solid Amazonian plates that I can trust to be clean.

---

Those Amazonian jars look so juicy, i'd probably eat all of them in one sitting..yummm

Quote:

Kloakk said:

05.02.2025

Still not much happening. Did more transfer two days ago, I am still not 100% happy with my plates of x7x+ and TWxPE6. I was going to do cabin sequestering for these T3's and T4's, but my lab "dealer" took they're sweet ass time sending my order so here we are, doing transfers just so they don't outgrow they're current home..

Well my order is on it's way and should arrive in a day or two. After reading MycCycle's journal I decided to not use agar as cabin roof, not going to get in to that here, but it just makes more sense to me and so I ordered different sized slides and will be using them when doing cabin sequestering, while we wait for that, some pictures.

x7x+ T3's



x7x+ T3 closeup


TWxPE6 T4's



TWxPE6 T4's closeup



x7x+ 12 days

---

eilauver said:

What’s with the intendation/hole? Haven’t stumbled on anything that talks about something like this yet, but it’s a second time I see it recently.

---

These are just packed a bit more on the out edges and center. I've been toying around with trying to direct/encourage growth patterns.

I think removing some bulk from the center of a large bed would probably allow better aeration and quick colonization for the rest of the bed.

In my mini-bog planters for carnivorous plants I inset an empty pot so I can see the water levels and allow aeration.