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Re: Let's All Grow Mushrooms 2025 [Re: Lilgrower]
    #29091904 -

Sounds like your agar is just a little soft. Not ideal for transfers, but once you're almost there it's really practical to make slurry / LI from. Nothing gets a jar started like inoculant dripping from the glass.

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Re: Let's All Grow Mushrooms 2025 [Re: Churpee Choozle] * 3
    #29091912 -

My cube transfer took and didn't contaminate, kinda surprised me. After all the effort I put in to trash that plate. Made another T1 two days ago.
Galindoi does nothing, also on BRF/verm.
The Gymnopilus print has been lost in transit for well over a month now.

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Re: Let's All Grow Mushrooms 2025 [Re: Churpee Choozle] * 4
    #29091948 -

1/17


Well, most of my pan cyan plates went bacterial.  my technique of going spore print to agar isn’t the most professional (I take a small bit of agar from the xacto cooling dish, rub it on spores, and then put into a sterile agar dish).  I could’ve stepped that up some, but live and learn.


One plate is showing a tiiiiny tiny bit of mycelium on it so I’m going to say my shroomy prayers and in 2-3 days hopefully take enough transfers to keep this train rolling

India Woodlover plates have yet to show any activity.  Keeping a close eye on them, though.  Only been like 4 days :smile:


--------------------

When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
- nick sand


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Re: Let's All Grow Mushrooms 2025 [Re: PinePalace] * 7
    #29092319 -

P
PinePalace said:
already transferred this rhizo but kinda crazy how distinct it now is... prob gonna get another T2 so im 100% sure i got it!



:feelsgoodman:



Yup, think i got it, from ~10% rhizo to now 50+% :smile:

Very excited for these charcoal glass T3 plates, should be throwin straight ropes. Gotta control myself and let them cool until the morning lol.

Used 6g agar, 4g LME, 3g charcoal, ~300ml water











1/18/25 (hopefully) last transfers complete


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:mushroom2: panta rhei :mushroom2:

Edited by PinePalace (01/18/25 07:02 AM)

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Re: Let's All Grow Mushrooms 2025 [Re: PinePalace] * 8
    #29093038 -


While my LAGM Neox spores didn't germ (which I re-streaked today) - the Stropharia alcis did.  The myc is a nightmare, and made me very suspicious at first.  It is radial on both germ dishes, and structured the same- which gives me hope.  It is more uniformly radial on T1s (example pictured against germ dishes) as well,  so there's that.  Fingers crossed.  Took some T2s today,  so we'll see if we go uphill or roll back down.  I notice that with a lot of random wild mushrooms you clone - the myc can be really random at times.  Even something like lion's mane or maitake tend to express irregular/non-uniform structures.  I hate using potato broth agar too - always gives weak ass growth which isn'thelping in this case :resignednonacceptance:
I'll be very happy if I manage to get wedges of this species to grain this month.

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Re: Let's All Grow Mushrooms 2025 [Re: PinePalace] * 2
    #29093111 -

Pretty sure I’ve gotten duped and I think I need to change my agar recipe haha. Been running Milkboys pda and it’s been good to me for the most part.
But I’m seeing a trend that I think needs to be addressed.

So for some context:
This is an ochraceocentrata wedge that I received, you can see how the mycelium didn’t start to look fuzzy and white until it grew out substantially. I thought because it was no pour and super soft..


Initially, it looked just like these, which are crimini clones taken from the sterile inner stipe tissue a few weeks ago. You can see the typical form of mycelial growth.



And now, all the “contam” that my lagm plates have showed me..







Now, I typically simmer my agar to help incorporate all the dry ingredients before pc’ing.
Which I didn’t with this batch.
There were still chunks of unincorporated dry material visible.
Maybe it was too soft as well?

Gonna make some xfers with a new batch and see what happens.


--------------------
For it is only Darkness that crafts one to thrive in the Light. As illumination of the soul, its brilliance unmatched.
               
                    🌕🌖🌗🌘🌑🌒🌓🌔🌕
  :sporedrop:Gymnopilus subspectibilis grow log:sporedrop:             
•89g fresh subspectibilis tea•
•Rapéh Crafters•
• Gift yourself a healthy dose of Self banning•

Edited by Soloist (01/18/25 10:57 AM)

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Re: Let's All Grow Mushrooms 2025 [Re: Dala] * 6
    #29093535 -

January 18, 2025

Made 4 T1's of AA+.  All 3 plates had germinated.

Went swab > 3 pucks with a fresh PE swab, as there was no action on the 3 agar germ plates.


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MUSHROOMS RULE

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Re: Let's All Grow Mushrooms 2025 [Re: Dala] * 6
    #29093963 -

1/18



India Woodlover and Pan Cyan Peace River spores appear to have germinated!  Very excited :smile: 

will update when I take transfers in a few days.  I dunno if this pan cyan plate is clean but it isn’t shleppin bacteria like the other ones so that’s kinda sweet

Keep up the good work people!


--------------------

When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
- nick sand


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Re: Let's All Grow Mushrooms 2025 [Re: Psicomb] * 1
    #29094118 -

Psicomb said:
1/17


Well, most of my pan cyan plates went bacterial.  my technique of going spore print to agar isn’t the most professional (I take a small bit of agar from the xacto cooling dish, rub it on spores, and then put into a sterile agar dish).  I could’ve stepped that up some, but live and learn.


One plate is showing a tiiiiny tiny bit of mycelium on it so I’m going to say my shroomy prayers and in 2-3 days hopefully take enough transfers to keep this train rolling

India Woodlover plates have yet to show any activity.  Keeping a close eye on them, though.  Only been like 4 days :smile:



I like flame sterilizing my #11 blade, place it on top of the closed receiving plate, wait a minute and then gently (using the blade tip) scrape off a few specks from the spore print into the plate in a manner that resembles a clam..the lid just half way open. Took me a lifetime to get to where i am now but for every decent grow i also had a million loses. Much love :heart::heart:


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:ancientaliens:

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Re: Let's All Grow Mushrooms 2025 [Re: astra] * 1
    #29094355 -



The cubensis T1s. The discoloration is soot from flaming the knife with a large candle, I know, that's not how it's supposed to go. Couldn't find the torch when the next day it was just standing on the table. Drugs r bad.
Galindoi is probably done for. This little pissdrop is all that grew out of my ancient spores. Important lesson: make fresh prints yearly. Learn freezer storage. I've grown this species since 2016, never used it more than say three times a year. It was however our absolute fav for dancing (high doses of Ps cyan are hit ir miss at crowded venues with 200 bpm music), but I fucked up on long term survivability.



A very kind member offered a swab of Gymnopilus, so my hopes of growing those are reinstated. I see you removed the offer from your post, so don't know if you want to be mentioned by name, but thanks for your generosity!

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Re: Let's All Grow Mushrooms 2025 [Re: Churpee Choozle] * 7
    #29094587 -

Vader T1s have grown out, took T2s today.



--------------------
This hobby has taught me many things.
Most of those things are patience.


The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
CakeWars
III

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Re: Let's All Grow Mushrooms 2025 [Re: Scemilope] * 5
    #29094593 -

Nat T1s:



Still trying to make time to re-swab WGT, as well as the prints I took from a couple of PE7s the other day.


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Let's All Grow Mushrooms 2025 [Re: Axman] * 7
    #29094607 -

Axman said:
1-19-25:


Grabbed a different Amazonian print, wet 4x swabs with sterilized distilled water, rolled them in the print, then broke them off and jammed into each puck. I also took the entire print and made a spore syringe with it. I used that syringe to also put some drops on the opposing side of each BRF puck.


I'm not convinced, but this might be some white fluff growing on this contaminated plate/swab.


Tico Rye jars are doing well. I did a break and shake on this jar after the picture was taken.



I'm fairly determined to get these spores to germinate, I'm going to see if I can find some rubber bands and glue the tip of the syringe shut to force hydrate it.

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Re: Let's All Grow Mushrooms 2025 [Re: Axman] * 11
    #29095136 -

Some of the main contenders showing promise. Bispo, Outdoor ttbvi, GFK and Minami Okinawa also joining the race :heart::heart:



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:ancientaliens:

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Re: Let's All Grow Mushrooms 2025 [Re: astra] * 7
    #29095205 -

Jan 20 Update!

So, I see what strikes me as Germination in one of the remaining 2 GT plates! It took a bit, likely a mix of stubbornness and cold temps. But it's something! Not the best pic, but I can see a few patches, roughly circular. There are other competitors on that plate, though. So I'll likely xfer to a "just in case" BRF puck as well as a couple of fresh plates.



The other GT plate is very quiet, still. But it looks to have the least absolute growth. But it may just be shy/undetectable my monkey eyes atm. We'll have to wait and see.



As for the surviving P. Djamor plate...



It's looking grim. Looks even worse IRL :sad: Prolly gonna toss that, start the spores again into a puck. On the bright side, it'll make my log a little simpler to just keep track of GT. Specially due to some unrelated projects creeping in (myco and otherwise).

T1s will have to wait until Wednesday due to some work/paperwork/busywork stuff. By then, both GT plates should give me more information to work off of. Feeling nervous, but excited!


--------------------
There is no season when you are grown
You are always risen from the seeds you've sown
There is no reason to rise alone
Other stories given have sages of their own

Greene's LAGM 2025 Log!
Current Projects![/url]

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Re: Let's All Grow Mushrooms 2025 [Re: GreeneMyco] * 8
    #29095389 -

I think one of my Pan cyan plates has started to germinate! Still trying to get a decent pic tho.


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Let's All Grow Mushrooms 2025 [Re: KlarnEvil9] * 3
    #29095416 -

KlarnEvil9 said:
I think one of my Pan cyan plates has started to germinate! Still trying to get a decent pic tho.



hell yeah congrats!
and are you trying to use a cell phone, a point and shoot camera, or a full size digital camera?

cellphone: download a camera app that lets you control focus manually. set it to a foot or two and move the plate back and forth until it's sharp. slightly over-expose as auto mode will try and even out the backlight and make the mycelium really dark.

point and shoot: not much can be done, enable macro mode if it has one.

full size: manual focus w/ macro lens, set the exposure to 'single point' on the agar and blast away. Preferably in RAW format.


--------------------
:mushroom2: panta rhei :mushroom2:

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Re: Let's All Grow Mushrooms 2025 [Re: PinePalace] * 1
    #29095420 -

I appreciate the info, that will be helpful!

I've been using a cell phone. The main issue is being able to see anything with backlighting, but a friend is coming by later to help me get it sorted.


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Let's All Grow Mushrooms 2025 [Re: KlarnEvil9] * 5
    #29096340 -

One side of the brf puck looking great. Something sinister ( I think cobweb ?) showed up on the other side. Took a couple transfers.




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Re: Let's All Grow Mushrooms 2025 [Re: KlarnEvil9] * 8
    #29096430 -

Pan cyan plate has finally germed!



--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Shop: Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy   MagicBag.co All-In-One Bags That Don't Suck


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