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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29013248 -

Are they all ms? I’d transfer from the far right one if I had to, otherwise they’d each get their own shoe


--------------------
Thank your postal workers when you see them. None of this would be possible without them.

Necessity is the mother of invention

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies] * 1
    #29013250 -

Huskies said:
Which of these Natalensis jars looks best for g2g? The middle one was my initial pick, but now that it has consolidated a bit and has these very thick ropey growths idk anymore.




Honestly, they ALL look a little bacterial. The first is the best of them all. I wouldn't use any for G2G though.

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Re: Ask Quick Questions, Get Quick Answers [Re: EniQma]
    #29013253 -

Nope. Some are from agar plates the others from my previous g2g on oats and was, which overall look fine to me but maybe I’m dumb


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29013310 -

Real quick question, y'all!

I got 8 trays for my dehydrator. I filled up 4 @ around midnight on 11/4.

Then around 5pm or so on 11/4 I filled the other 4 trays and put them underneath the first 4 in the dehydrator.

My question is should the top 4 trays be dry in spite of fresh ones being added below them? It's now about noon on 11/5 so the top 4 had 36 hours to dry.

On a related note...would I be able to take the bottom 4 trays, make them the new top 4, refill the bottom 4, and empty the NEW top 4 (previously the bottom 4) once they hit 24 hours?

Basically can a dehydrator be rotated in such a way that fresh mush can be added before a "cycle" is complete without prolonging the amount of time needed for ones that were partially dried when fresh were added???

Sorry if this is phrased all wonky, just tryna be specific about my situation.

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Re: Ask Quick Questions, Get Quick Answers [Re: RockinRobot] * 1
    #29013327 -

RockinRobot said:
Huskies said:
Which of these Natalensis jars looks best for g2g? The middle one was my initial pick, but now that it has consolidated a bit and has these very thick ropey growths idk anymore.




Honestly, they ALL look a little bacterial. The first is the best of them all. I wouldn't use any for G2G though.


I generally believe 100% that if I dont give up, I will always figure it out, but I'm not sure that I will ever be able to see bacterial spawn and it's getting to the point of ridiculous frustration. ReverendMyc sent me to a fantastic post, and I understand the pressed grains with bare spots and condensation, but Ive never seen any of the blatant signs like goopy shit or creamy myc or death stars, barren patches of grain, etc. My plates look clean and I've even poked and taken to brf - if I'm still fuckin up, it's gotta somehow be my a2g and I dont get it. Sorry for the rant, but this comment kicked me in the nuts bc I dont see it. Im tired of running so many shoeboxes due to the workload involved, but this where I'm at until I figure out where I'm being sloppy. Ugh...


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #29013354 -





When I said plates that look like this, how the hell is my spawn still coming out bacterial? I typically slice them up into 9-12 pieces and flick them into the jar. The plate is maybe somewhat hovering over the jar, but I keep it to a minimum, and idk how else one would get the shit in there without having them close together. This is all done in front of an ffu - I keep an upside down qt jar in first air off to the side where I set the jar lid down(upside down) while I inoculate. I flame sterilize my scalpel and even run the first couple inches of the handle through the flame a bit, just as added precaution. I initially thought maybe I was cracking my unmodded jar lids too much, so I abandoned that in favor of SFD cutouts, which get a ring of silicone on the underside and around the edge. I've never felt like I was being so thorough, yet somehow still fuckin things up and this has been months of trying to get this right. Im ditching wbs too - fuck this mixed seeds and corn. Done with it. I've done 50min boiling water in a bucket tek (sorry, can't remember who wrote it), these last 2 rounds I did no soak simmer (hamloaf?), Ive done 24hr soak, load wet and PC, I did a test run on Yoshis no prep which came out too wet...I'm about to pull my fuggin hair out. Ive got to be letting contaminates in when I inoculate- theres literally nothing left, and idk what to do about it. Maybe just take a small piece I can pitch into the jar real quick and reduce exposure time to next to nothing? Prepping shoeboxes for 4 jars is a PITA and I want to move on to bigger tubs and g2g, but I just feel stuck as hell right now. I'm not even necessarily looking for an answer to a question- I dont even know what the hell to ask, at this point. Sorry for the rant - that defeated feeling is creeping in. Maybe time for a break, idk.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 2
    #29013367 -

WBS

rinse well

Soak

Eliminate floaters/corn (kinda optional but I prefer it)

Rinse well again

Spread out on towel (kinda optional)

Load up

PC a MINIMUM of 105 minutes, a full 2 hours wouldn't hurt (these times are for jars not bags)

Make sure you're venting the PC for at least 10-15 minutes before bringing up psi


Personally I think your plates look great. It's probably a grain sterilization issue.

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Re: Ask Quick Questions, Get Quick Answers [Re: Winston Smith] * 1
    #29013377 -

I rotate my mushrooms in the dehydrator. I try to keep the drier ones closer to the fan and heating element, but I doubt it makes a difference. I almost always run MS so I tend to have shit coming in at different times.

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Re: Ask Quick Questions, Get Quick Answers [Re: Winston Smith] * 4
    #29013383 -

Plates look good. You already know where the problem is and how to fix it cuz you said it.

Not sure how you're "flicking" multiple pieces of agar into a jar, but that doesn't sound sterile. Do what you said and use one wedge per jar, size doesn't matter, sterile tek does.


--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: Ask Quick Questions, Get Quick Answers [Re: Winston Smith]
    #29013387 -

Winston Smith said:
WBS

rinse well

Soak

Eliminate floaters/corn (kinda optional but I prefer it)

Rinse well again

Spread out on towel (kinda optional)

Load up

PC a MINIMUM of 105 minutes, a full 2 hours wouldn't hurt (these times are for jars not bags)

Make sure you're venting the PC for at least 10-15 minutes before bringing up psi


Personally I think your plates look great. It's probably a grain sterilization issue.


The only thing I havent done there is rinse again after the soak, dry after soak, or remove corn, which I have a minnow net that I think would be perfect for such a thing, if I can't source another grain next go around.

Rinsed and removed floaters

Soaked 24hr

*didnt dry* and loaded jars

Vent 10min with the timer starting when the locking pin engaged.

Sterilize a full 2hrs without exception.

Half the time Ive left them until room temp and half the time Ive pulled them when the lock disengaged.

Ive only run it like that twice, but the grains did come out less swollen, lighter in color, and overall not as wet looking.

Last go around I rinsed and removed floaters, put on the stove, brought to 200 degrees, reduced heat to medium and stirred for 5min. Strained in brewer bag and then left them spread out until room temp before PC cycle. I only loaded the jars half full bc I have gotten quite a bit of expansion with past runs, but they didnt expand any on this one and there wasn't much for signs of excess moisture or burst grains, so idk...maybe that was the best Ive done. Ill nocc one up tonight and see how she goes. Appreciate the feedback brotha.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: LadysKnight] * 2
    #29013402 -

LadysKnight said:
Plates look good. You already know where the problem is and how to fix it cuz you said it.

Not sure how you're "flicking" multiple pieces of agar into a jar, but that doesn't sound sterile. Do what you said and use one wedge per jar, size doesn't matter, sterile tek does.


Idk if "flicking" was an accurate description, but I'm picking up what you're laying down. I was basically following beefs video on a2g. Slice plate and push into the jar with the scalpel, but I'm going to try just a single wedge and see if that does the trick. More importantly, I'm gonna get some sloppy toppy tonight and meditate on the fact that this is a hobby and I need to keep my ego from pushing me into frustration. I rarely struggle for this long, but I need to keep my shit in check too. Appreciate ya, amigo.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 1
    #29013416 -

ThurmanMurman said:
RockinRobot said:
Huskies said:
Which of these Natalensis jars looks best for g2g? The middle one was my initial pick, but now that it has consolidated a bit and has these very thick ropey growths idk anymore.




Honestly, they ALL look a little bacterial. The first is the best of them all. I wouldn't use any for G2G though.


I generally believe 100% that if I dont give up, I will always figure it out, but I'm not sure that I will ever be able to see bacterial spawn and it's getting to the point of ridiculous frustration. ReverendMyc sent me to a fantastic post, and I understand the pressed grains with bare spots and condensation, but Ive never seen any of the blatant signs like goopy shit or creamy myc or death stars, barren patches of grain, etc. My plates look clean and I've even poked and taken to brf - if I'm still fuckin up, it's gotta somehow be my a2g and I dont get it. Sorry for the rant, but this comment kicked me in the nuts bc I dont see it. Im tired of running so many shoeboxes due to the workload involved, but this where I'm at until I figure out where I'm being sloppy. Ugh...


Same, my plates have uniform growth without any bacterial blobs. I do everything under the sab, if my technique was complete shit my poured plates or transfers would contaminate but they don't, especially when left alone.

Either its a case of bacteria has been going along the entire time since the spores in such a passive way that it only fucks up when going to bulk, or I am fucking up when going to grain with agar or g2g.

Some condensation can be due to just the environment in the jar, especially once it mycelium takes over and shrinks it a tiny bit, so some humidity gets in between the grain and class.

If someone can help identify WHAT makes you think bacteria besides the condensation, because I do not know what to look for it seems.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: Winston Smith]
    #29013421 -

Winston Smith said:
Real quick question, y'all!

I got 8 trays for my dehydrator. I filled up 4 @ around midnight on 11/4.

Then around 5pm or so on 11/4 I filled the other 4 trays and put them underneath the first 4 in the dehydrator.

My question is should the top 4 trays be dry in spite of fresh ones being added below them? It's now about noon on 11/5 so the top 4 had 36 hours to dry.

On a related note...would I be able to take the bottom 4 trays, make them the new top 4, refill the bottom 4, and empty the NEW top 4 (previously the bottom 4) once they hit 24 hours?

Basically can a dehydrator be rotated in such a way that fresh mush can be added before a "cycle" is complete without prolonging the amount of time needed for ones that were partially dried when fresh were added???

Sorry if this is phrased all wonky, just tryna be specific about my situation.


Don’t do this.  Do one batch at a time or they could just be taking in moisture being put off from the fresh ones.

One batch at a time, highest temp setting, 24 hours min.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies] * 1
    #29013424 -

Huskies said:
ThurmanMurman said:
RockinRobot said:
Huskies said:
Which of these Natalensis jars looks best for g2g? The middle one was my initial pick, but now that it has consolidated a bit and has these very thick ropey growths idk anymore.




Honestly, they ALL look a little bacterial. The first is the best of them all. I wouldn't use any for G2G though.


I generally believe 100% that if I dont give up, I will always figure it out, but I'm not sure that I will ever be able to see bacterial spawn and it's getting to the point of ridiculous frustration. ReverendMyc sent me to a fantastic post, and I understand the pressed grains with bare spots and condensation, but Ive never seen any of the blatant signs like goopy shit or creamy myc or death stars, barren patches of grain, etc. My plates look clean and I've even poked and taken to brf - if I'm still fuckin up, it's gotta somehow be my a2g and I dont get it. Sorry for the rant, but this comment kicked me in the nuts bc I dont see it. Im tired of running so many shoeboxes due to the workload involved, but this where I'm at until I figure out where I'm being sloppy. Ugh...


Same, my plates have uniform growth without any bacterial blobs. I do everything under the sab, if my technique was complete shit my poured plates or transfers would contaminate but they don't, especially when left alone.

Either its a case of bacteria has been going along the entire time since the spores in such a passive way that it only fucks up when going to bulk, or I am fucking up when going to grain with agar or g2g.

Some condensation can be due to just the environment in the jar, especially once it mycelium takes over and shrinks it a tiny bit, so some humidity gets in between the grain and class.

If someone can help identify WHAT makes you think bacteria besides the condensation, because I do not know what to look for it seems.


Growth that isn’t homogeneous, sweaty jar, really anything that doesn’t look “just right.”

All it takes is a single rod floating in and you’ve got bacteria.  Most of the time it doesn’t show up on the plate in a visible colony, or it gets in when transferring.

Never eliminate your SAB work as a contamination vector, hubris never fails to be punished.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #29013428 -

Mr Piggy said:
Winston Smith said:
Real quick question, y'all!

I got 8 trays for my dehydrator. I filled up 4 @ around midnight on 11/4.

Then around 5pm or so on 11/4 I filled the other 4 trays and put them underneath the first 4 in the dehydrator.

My question is should the top 4 trays be dry in spite of fresh ones being added below them? It's now about noon on 11/5 so the top 4 had 36 hours to dry.

On a related note...would I be able to take the bottom 4 trays, make them the new top 4, refill the bottom 4, and empty the NEW top 4 (previously the bottom 4) once they hit 24 hours?

Basically can a dehydrator be rotated in such a way that fresh mush can be added before a "cycle" is complete without prolonging the amount of time needed for ones that were partially dried when fresh were added???

Sorry if this is phrased all wonky, just tryna be specific about my situation.


Don’t do this.  Do one batch at a time or they could just be taking in moisture being put off from the fresh ones.

One batch at a time, highest temp setting, 24 hours min.


So uh...lets say a guy has been doing this. Not me of course, but a guy...is it worth running them through the dehydrator again? They've been in mason jars since, and it hasn't been that long. Snap test seemed on point. Mostly nats, so skinnier than cubes, for what that's worth. Also, what would you recommend one do with the batch that's on deck? Harvest late or paperbag in fridge maybe?


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies] * 1
    #29013444 -

Huskies said:
ThurmanMurman said:
RockinRobot said:
Huskies said:
Which of these Natalensis jars looks best for g2g? The middle one was my initial pick, but now that it has consolidated a bit and has these very thick ropey growths idk anymore.




Honestly, they ALL look a little bacterial. The first is the best of them all. I wouldn't use any for G2G though.


I generally believe 100% that if I dont give up, I will always figure it out, but I'm not sure that I will ever be able to see bacterial spawn and it's getting to the point of ridiculous frustration. ReverendMyc sent me to a fantastic post, and I understand the pressed grains with bare spots and condensation, but Ive never seen any of the blatant signs like goopy shit or creamy myc or death stars, barren patches of grain, etc. My plates look clean and I've even poked and taken to brf - if I'm still fuckin up, it's gotta somehow be my a2g and I dont get it. Sorry for the rant, but this comment kicked me in the nuts bc I dont see it. Im tired of running so many shoeboxes due to the workload involved, but this where I'm at until I figure out where I'm being sloppy. Ugh...


Same, my plates have uniform growth without any bacterial blobs. I do everything under the sab, if my technique was complete shit my poured plates or transfers would contaminate but they don't, especially when left alone.

Either its a case of bacteria has been going along the entire time since the spores in such a passive way that it only fucks up when going to bulk, or I am fucking up when going to grain with agar or g2g.

Some condensation can be due to just the environment in the jar, especially once it mycelium takes over and shrinks it a tiny bit, so some humidity gets in between the grain and class.

If someone can help identify WHAT makes you think bacteria besides the condensation, because I do not know what to look for it seems.


I think I've previously underestimated how sneaky bacteria can be on agar, but since getting much pickier about my plates, I'm pretty sure I'm fuckin up when I inoculate. There's not really anything left. If anything, my grain is over hydrated, and my PC cycles are on point, my lids are solid...my technique is all thats left and the only variable that requires any real skill too. 🤣🤣 We'll get there man...I hope.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 1
    #29013456 -

...perhaps my lids are not enough. When I use the standard ring ones, I flip them over before I make the whole. SOme of them have been used a lot, I straighten them out if i notice they are bumpy.

Now, if its bacteria during my grain incolucation, would Lysol help sterilize inside the SAB? Spray, cover the holes, wait 15 mins, and then after alcoholign the crap out of my hands and arms, do the transfers?


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

Edited by Huskies (11/05/24 03:10 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 1
    #29013467 -

Quote:
I think I've previously underestimated how sneaky bacteria can be on agar, but since getting much pickier about my plates, I'm pretty sure I'm fuckin up when I inoculate. There's not really anything left. If anything, my grain is over hydrated, and my PC cycles are on point, my lids are solid...my technique is all thats left and the only variable that requires any real skill too. 🤣🤣 We'll get there man...I hope.


Man, I feel your pain. I'm glad you post a bunch of detailed stuff here, most of it applies to my grows - or it will soon. I read all the Q's and A's and pay attention, but I see the lack of refinement in my own sterile technique. We will figure it out!


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Ask Quick Questions, Get Quick Answers [Re: KlarnEvil9]
    #29013510 -

KlarnEvil9 said:
Quote:
I think I've previously underestimated how sneaky bacteria can be on agar, but since getting much pickier about my plates, I'm pretty sure I'm fuckin up when I inoculate. There's not really anything left. If anything, my grain is over hydrated, and my PC cycles are on point, my lids are solid...my technique is all thats left and the only variable that requires any real skill too. 🤣🤣 We'll get there man...I hope.


Man, I feel your pain. I'm glad you post a bunch of detailed stuff here, most of it applies to my grows - or it will soon. I read all the Q's and A's and pay attention, but I see the lack of refinement in my own sterile technique. We will figure it out!


Another realization that kinda ties in - agar is the best tool we have for sure, but it's not a picture perfect window into the culture and still requires us to hone our skills in reading the plate. Obvious contamination is obvious, but I absolutely went in with the idea that if myc was growing on any given spot, it must be good bc how could it grow there if something else had colonized that particular spot, right? Wrong. Lmao Study and practice and lots of it.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 1
    #29013519 -

ThurmanMurman said:
KlarnEvil9 said:
Quote:
I think I've previously underestimated how sneaky bacteria can be on agar, but since getting much pickier about my plates, I'm pretty sure I'm fuckin up when I inoculate. There's not really anything left. If anything, my grain is over hydrated, and my PC cycles are on point, my lids are solid...my technique is all thats left and the only variable that requires any real skill too. 🤣🤣 We'll get there man...I hope.


Man, I feel your pain. I'm glad you post a bunch of detailed stuff here, most of it applies to my grows - or it will soon. I read all the Q's and A's and pay attention, but I see the lack of refinement in my own sterile technique. We will figure it out!


Another realization that kinda ties in - agar is the best tool we have for sure, but it's not a picture perfect window into the culture and still requires us to hone our skills in reading the plate. Obvious contamination is obvious, but I absolutely went in with the idea that if myc was growing on any given spot, it must be good bc how could it grow there if something else had colonized that particular spot, right? Wrong. Lmao Study and practice and lots of it.


I just discovered that all but 1 of my outdoor fruit clone plates are SUPER CONTAM-A-NACIOUS!!!! I'll get some pics in a bit, they are pretty nasty. And one of my plin plates has some dark looking nasty growing on the side... right above the plin! :foreheadslap:

So much SAB work to do tonight, and most of the new plates I made are still full of condensation... at least the outdoor bed is still jamming.


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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