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OfflineTaz
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Registered: 03/19/01
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Re: Making truffles
    #289503 - 04/10/01 10:48 AM (21 years, 5 months ago)

captainmax, shouldn't you be over at the new forum advertising for yourself are something?



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Offlinenononsense
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Registered: 03/26/01
Posts: 319
Last seen: 20 years, 9 months
Re: Making truffles
    #289581 - 04/10/01 01:15 PM (21 years, 5 months ago)

What do you call them than? Sclerotia? Look I've heard/red something about tampanensis which cultivated with straw or grass seeds, in dark without casing in the jar who made psychedelic truffles!
So no gourmet edible this time, for the trip that's my intention. So, can anyone give any info to me about this. Can I clone a fresh one to agar, and use the mycelium for inoculating jars? Or don't they clone that way?
Thanks

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Offlinenononsense
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Re: Making truffles [Re: nononsense]
    #290296 - 04/11/01 08:35 AM (21 years, 5 months ago)

hello?
can someone help me?

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Invisibleslither
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Registered: 02/03/01
Posts: 365
Re: Making truffles [Re: nononsense]
    #290301 - 04/11/01 08:44 AM (21 years, 5 months ago)

You need tamps or Mexicana's, Not Cubensis, I've had something similar to sclerotia with cubies, but it's not the same. They're supposed to be fairly difficult to achieve, but www.igosky.com/mexicana/ has some good info on cultivating P. Mexicana's.



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Offlinenononsense
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Re: Making truffles [Re: slither]
    #290307 - 04/11/01 08:50 AM (21 years, 5 months ago)

Yeah, that's what I said, the tamp. were used to make em, when not removed out of the jar!


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Offlinenononsense
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Re: Making truffles [Re: nononsense]
    #290310 - 04/11/01 08:54 AM (21 years, 5 months ago)

I just wanna know if I can clone one from a fresh sclerotia!

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Offlinehatter
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Re: Making truffles [Re: nononsense]
    #290314 - 04/11/01 08:59 AM (21 years, 5 months ago)

Dude, truffles take six years to grow...yes, 6 years. And you can't just grow them, either. They grow from the root systems of a few kinds of trees.

They are 2500/lb. for a reason...they are rare and hard to cultivate. They train pigs to find truffles they are so hard to find(coincidentally, the pigs will fight you for the lil guys!).

However, there are a few places that sell the tree seedlings with already innoculated roots...just do a search, I'm sure you will find them.

If you can successfully cultivate them, there is huge money...like I said, around 2500/lb.!!



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Offlinenononsense
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Re: Making truffles [Re: hatter]
    #290315 - 04/11/01 09:01 AM (21 years, 5 months ago)

So than I mean sclerotia, I'm a newbeeeeeeeee, so...


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InvisibleThorA
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Re: Making truffles [Re: nononsense]
    #290327 - 04/11/01 09:10 AM (21 years, 5 months ago)

This post belongs in the Gourmet and edible shrooms forum..... Like Captain Max has already said.....

I could go for some truffles, mmmm mmm good :smile:



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Offlinenononsense
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Making sclerotia from tampanensis [Re: Thor]
    #290329 - 04/11/01 09:14 AM (21 years, 5 months ago)

Better?

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InvisibleThorA
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Re: Making sclerotia from tampanensis [Re: nononsense]
    #290333 - 04/11/01 09:17 AM (21 years, 5 months ago)

LOL.... yep, much better... :smile:



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Offlinenononsense
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Re: Making sclerotia from tampanensis [Re: Thor]
    #290336 - 04/11/01 09:19 AM (21 years, 5 months ago)

Thor, can you help me out. Because the sclerotia are sold here fresh in smart shops, I can obtain a fresh one, can I clone these sclerotia on agar????
Thanks!

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Invisibleslither
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Registered: 02/03/01
Posts: 365
Re: Making sclerotia from tampanensis [Re: nononsense]
    #290383 - 04/11/01 11:50 AM (21 years, 5 months ago)

No, you cannot clone sclerotia, well you may be able to clone it, but you still have to pray for it. Not very many people have been able to get it to form in the jars like stamets pictures in TMC, I have heard of a few having luck with it forming on their casing layer. There is a fairly active thread on this at drool donkey, I think it's under experimentation.



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Offlinenononsense
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Re: Making sclerotia from tampanensis [Re: slither]
    #290386 - 04/11/01 11:53 AM (21 years, 5 months ago)

So I should buy a Tampanensis print, put spores on agar, put mycelium in jars and let stand in the darK????

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Invisibleslither
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Re: Making sclerotia from tampanensis [Re: nononsense]
    #290494 - 04/11/01 06:39 PM (21 years, 5 months ago)

If you have access to fresh and are familiar with use of agar, then clone it, I'm sure you'll get mycellium, just don't count on getting sclerotia to form. Good luck! Like I said, go to Experimentation at www.drooldonkey.org , there's an active thread there mainly concerning mexicana, but basically the same principle, I've never done this or attempted it, only read about it, and I'm the only one answering your questions, so you might be in the wrong place. Or at least try advanced cultivation forum here.



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Anonymous

Re: Making sclerotia from tampanensis
    #290978 - 04/12/01 03:41 AM (21 years, 5 months ago)

Of course you can clone it. It's made of mycelium just like mushies. If it is fresh it will be easy, if it is dry already, rehydrate it. After rehydrating it will get fuzzy white. Under as sterile conditions as possible, cut a piece off and put it on fresh agar solution in a petri or sterile jar. Due duplicates !!!!!! Once the mycelium grows out, transfer to fresh plates. Watch for possible contaminants. Transfer away from these, until you have several pure cultures. Sterilize 1 cup ryegrass seed : 1/2 cup water soaked overnight and shaken really good, for 1 hour at 15 psi. Let jars cool overnight, shake like hell again, and inoculate with pure cultures under sterile environment. Shake like hell again. Wait untill mycelium is visible growing at 80 degrees Far., give or take a few degrees. Shake again. After at most two weeks or so, they should be colonized. Place in a storage bin, in darkness, and let them vegetate for months, up to six months. Check when ever you like, keep a look out for contaminants. You will see sclerotia forming at the walls of the jars in as little as three weeks. They will multiply and grow over time. Tampanensis is not hard. The mexicana post at drool donkey is not about tampanensis. The mexicana spore race from sporeworks has yet to yield sclerotia invitro. But they have a syringe of tampanensis that is working fabulous. But hey you have fresh sclerotia, it's a guranteed success. Just clone, and practice a good sterile technique and you should have no problem.



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Offlinenononsense
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Registered: 03/26/01
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Re: Making sclerotia from tampanensis [Re: ]
    #291228 - 04/12/01 08:53 AM (21 years, 5 months ago)

Thanks you all that's the info I required. I have a very nice possibility to make agar very sterile. I can obtain a fresh example. So this should be easy to clone, I have done this with mushies also and got myself a nice pure culture. So I can start this with a sclerotia also. Did you say rye grass seed? Is this just plain rye or is this rye mixed with grass seed??
Keep temp in all stages around 80 F?
Thanks again!


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Don't look at me, I didn't do it!!


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Offlinenononsense
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Re: Making sclerotia from tampanensis [Re: nononsense]
    #292218 - 04/13/01 07:17 PM (21 years, 5 months ago)

I think the rye grass seed is not completely understood by me --> explain........!

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Offlinetom
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Registered: 05/28/00
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Re: Making sclerotia from tampanensis [Re: nononsense]
    #292229 - 04/13/01 08:05 PM (21 years, 5 months ago)

Move this to gourmet. Truffles are big change but m\not psychedelic.

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