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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Updated PF Tek Guide [Re: Ziran] * 1
    #28871854 -

I followed this guide to a t and got my first flush off a cake a couple of days ago. Thank you for doing this from the far future.

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Re: Updated PF Tek Guide [Re: RRyder420] * 1
    #28880463 -

This writeup is king


--------------------
The PF Tek is my area.:oldman:

Where all anonymity is lost, there cannot exist a person.

Where one cannot avoid to share, there is God

www.zamnesia.nl
RustyWhyte is Commercial!



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Re: Updated PF Tek Guide [Re: Cakewonder] * 1
    #28883115 -

Hello !

Thanks for the guide !
Just to be sure :

1. You do not put any kind of microtape / filter on the lid's holes.

2. You put like what, 2mm of syringue's solution per jar ?

Cheers !


--------------------
wow

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Re: Updated PF Tek Guide [Re: Tropical]
    #28883341 -

The dry non nutritious vermiculite is the filter, tape isn’t necessary.
A drop or two per hole so it depends on how many holes you have in your lids. 2 mL per jar is more than is required. I can inoculate 20 jars with a 10 mL syringe.

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Re: Updated PF Tek Guide [Re: The Tao]
    #28892851 -

Hey thanks for this guide… I followed along closely, the only difference is I’m not inoculating with a syringe but from agar chunks so the only thing I didn’t do was put a dry vermiculite layer.

When opening the jar I found the brew was ‘sticky’ and it was hard to get chunks in body of jar.

Felt I had the right field capacity according to the guidelines.

Ill be continuing to work with agar…but now I’m thinking perhaps this method is not best for transferring from agar.

Couple of questions:

Is it normal for it to be ‘sticky?’

Is this PF-tek good for agar transfers or should I be looking at an alternative tek?

Did some research and turns out PF not the best Tek for agar transfers… I’ll switch to grains if that’s better?

Edited by salubrious (07/30/24 01:57 AM)

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Re: Updated PF Tek Guide [Re: salubrious] * 3
    #28894973 -

Thanks for all the help boys n girls.

First mushroom ever -



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Re: Updated PF Tek Guide [Re: Professor Chaos]
    #28895034 -

Damn, great first!


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Updated PF Tek Guide [Re: Mr Piggy]
    #28900679 -

hia, I've currently got some jars on the go. the mycelium started appearing but has subsequently stalled. these jars were inoculated about 5 weeks ago. mycelium appeared after a week witb the growth yoi now see in about another week to 10 days, but since then nothing more. is there any way to move them along or do I ditch them and start again?

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Re: Updated PF Tek Guide [Re: Fuzzyness]
    #28900744 -

Why are there filters on the lids? Did you prepare the jars or was this a purchased kit?

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Re: Updated PF Tek Guide [Re: The Tao]
    #28901087 -

I prepared them myself.

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Re: Updated PF Tek Guide [Re: Fuzzyness]
    #28901112 -

What are the filters for?

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Re: Updated PF Tek Guide [Re: The Tao]
    #28901174 -

the lids have two holes in them which I inject the liquid through. i put a filter on to cover them up.

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Re: Updated PF Tek Guide [Re: Fuzzyness]
    #28901188 -

My lids have 5 holes and I don’t tape or filter them. Why are you? Do your jars have a dry vermiculite layer?

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Re: Updated PF Tek Guide [Re: The Tao]
    #28901206 -

yes they have a dry vermiculite layer at the top. when I've not covered the holes I've just ended up with green mould so I decided to minimise contamination risk by putting something over them. the last time I did it I used the same set up and managed to colonise the whole jar. why is covering them an issue?

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Re: Updated PF Tek Guide [Re: Fuzzyness]
    #28901220 -

I think this may be a sterilization issue. The verm filter if done correctly should be enough to filter any contams that aren't in your syringe already. Maybe next time keep a control jar to make sure.

EDIT: To clarify I mean the mold is a sterilization issue. The Tao is right that your current jars are probably stalling cuz of not enough air.

Edited by Pennock (08/05/24 05:28 PM)

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Re: Updated PF Tek Guide [Re: Fuzzyness]
    #28901228 -

I have five open holes in my lids supplying air. You have two holes with filters. One of the reasons jars stall is lack of air.

Edited by The Tao (08/05/24 05:40 PM)

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Re: Updated PF Tek Guide [Re: The Tao]
    #28901238 -

The dry verm layer works well as a filter. The cakes do need the holes for air.

Sounds more like you didn't sterilize the uncovered ones completely, especially if you had mold in the whole batch.

I run PF cakes in these boxes:



The dry layer works well enough to keep them from contamination even with 9 holes in them, they stay uncovered the whole time. I'll get an occasional mold spot close to an injection point, which just points to the needle bringing it in.

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Re: Updated PF Tek Guide [Re: LyleChipperson]
    #28901248 -

Is there a tek somewhere for that Lyle? Interested in details possibly for my next project.


--------------------
Hitchhiker's Guide to the Shroomery


“Once in a while you get shown the light, in the strangest of places if you look at it right.”

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Re: Updated PF Tek Guide [Re: Pennock]
    #28901428 -

Pennock said:
I think this may be a sterilization issue. The verm filter if done correctly should be enough to filter any contams that aren't in your syringe already. Maybe next time keep a control jar to make sure.

EDIT: To clarify I mean the mold is a sterilization issue. The Tao is right that your current jars are probably stalling cuz of not enough air.


Here is your answer.

The whole reason for the verm layer on top is to act as a filter.

You need to look at your sterilization time/methods/technique.

The whole reason PF works is the dry verm on top.

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Re: Updated PF Tek Guide [Re: Bigdogg]
    #28901614 -

I kept a control jar clean for 3 weeks (or something like that) If you dont have enough gas exchange the mycelium wont grow. Your probably putting too much on the top there.

Ive run jars with no verm layer, but then again I keep perfecting my sterilization so thats your problem, but even though I have filters in my plastics I still add a dry verm layer and the mycelium chews through that as well.


--------------------
The PF Tek is my area.:oldman:

Where all anonymity is lost, there cannot exist a person.

Where one cannot avoid to share, there is God

www.zamnesia.nl
RustyWhyte is Commercial!



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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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