tryptkaloids said:

How much clearance does a ffu need

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Bigworm said:

Nanutzu said:

I hope this is the right place to ask.

Quick background info

I transfered agar to 4 jars. (PC ed for 1h40min and left to cool over night)

Transfered agar to all 4.

Kept at temperature of around 20 to 25, in complete darkness.

2 of the jars got moldy within a week, no micellyum growth. (Same mold as picture below)

1 jar got mold the next week, almost no mycelium growth.

Last jar, looked perfectly clean with decent mycelium growth during these 2 weeks.

Now, 3rd week half a jar colonized, started to see grains getting moldy.

Few days ago, I turned the heating on and temperature jumped to ++30.

Noticed increased speed in mycelium growth, grew in 3 days as much as in 10.

Also noticed grains started to develop mold.

Can this still be used? or is kaput?






PS
He's a good dog.

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To be honest, the myc looks to be very rhizo. Usually rhizo is good but too rhizo could mean it's running from something funky. From my experience with it would seem it is either your grain prep or steril technique if your agar was clean. Do you have any pics of the plates?

P.S. Oats have done me wrong too many times for me to ever use again. It didn't matter if I pc'd for 3 hours @ 18psi they always had issues.

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tryptkaloids said:

Nanutzu said:

SwabMarley said:

Mould=kaput

Those jars are fuckin FULL!

Leave some headroom in jars for shaking and air exchange.

The mould I would guess came from your grain touching your lid.

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I know, I did left what I thought to be enough but clearly not not nearly enough.

But the mold is at the middle of the jar, as they are ~full how could that be the case? The mold would be at the top.

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What type of filter you using? Were they covered in foil? what did the agar look like?

The mold wouldn't be from the grain touching the filter, but a saturated filter could cause mold to get through depending on the filter

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tryptkaloids said:

Nanutzu said:

SwabMarley said:

Mould=kaput

Those jars are fuckin FULL!

Leave some headroom in jars for shaking and air exchange.

The mould I would guess came from your grain touching your lid.

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I know, I did left what I thought to be enough but clearly not not nearly enough.

But the mold is at the middle of the jar, as they are ~full how could that be the case? The mold would be at the top.

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What type of filter you using? Were they covered in foil? what did the agar look like?

The mold wouldn't be from the grain touching the filter, but a saturated filter could cause mold to get through depending on the filter

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The filter I used.



The agar was clean, I made sure to take only the healthy part.

DERRAYLD said:

Sorry but your recipe alone tells me you don't actually know if your agar was clean and then you add that the temperature caused the issue

Based on those jars either your agar was not clean or you totally messed up on the pc'ing.

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FriedEgg said:

aside from things like sterile error while inoculating or your agar being bad...  those oats look way too dry. they should be plump with moisture. bite one in half and see if it's white inside or clear. if you don't hydrate your grain, it won't sterilize properly. usually you have to simmer grain or soak it in very hot water for a day.
the mycelium looks fine though. it's not too rhizo. you'll know the myc is stressed if there's really long strands of individual rhizo growth. i don't see that in your photo.

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DERRAYLD said:

You didn't pc your jars correctly, also what are those filters?

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FriedEgg said:

he PCd correctly (PC ed for 1h40min and left to cool over night). but he didn't prep the grain correctly.

yeah soaking grain in cold water for 4 hours and then sterilizing it aint gonna cut it. just throw it away now and start over. simmer your grain for as long as you need to achieve full moisture content inside the kernel. the kitchen towel method is trash IMO. bite a kernel in half and look at the inside of the grain.


and yeah wtf are those filters? i don't even use filters on my jars. i just tighten the lid and then back it off about 1/16th of a turn and set it on a shelf without touching it. i've done lots of jars and it works fine that way. but that might not be possible with your pickle jar style lids. i use plastic canning lids.
if you really want to use filters, buy synthetic filter discs or if SFDs are unavailable, you can stuff polyfill REALLY tight into a small 0.5cm hole in the lid. use a chopstick or something to jam it in the hole. it's a really old method but it works if you have nothing else.

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DERRAYLD said:

Definitely don't spawn a dirty jar

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DERRAYLD said:

Use polyfil, the inside of a pillow.

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DERRAYLD said:

You can't pc the way you're doing it.
FIgure out what pressure you're pc'ing at first and ensure you're venting.

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DERRAYLD said:

He doesn't know what psi he's pcing at, that in itself is a glaring issue.
Chances are , and this is an assumption,he has a pc that is not even reaching 12psi.
If he can only get those filters then he's most likely in a developing country or European and I know pcs are an issue around the world.

No venting and not pc'ing at 15psi for 1.3 minimum is going to cause early mold in jars which we see here.

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I don't understand what you mean by venting. I had holes in the jars, covered by that filter.

I don't know how to verify the pressure. The cooker is quite old, made of solid material, quite heavy. Has 2 valves, one of them was releasing steam the other no.

I do have a small problem finding the right filters, I'll look and order online for the next batch.

This is it.

FriedEgg said:

oh shit well there he said it. i didn't see him say that earlier.

you can buy a pressure gauge and install it on the PC. i know you probably cant buy from amazon at your location but maybe you could find a pressure gauge from some other piece of equipment and install it on your PC?
something like this: https://www.amazon.com/Measureman-Liquid-Pressure-0-60psi-Stainless/dp/B07T43LMM6/

edit: can you take a photo of your PC?

venting means expelling all the gas in your PC before bringing it up to pressure. you're trying to expel all the air in your PC before actually steam sterilizing what's inside of it.

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