Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

MushroomMan Mycology
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: 1
Reviving a 2 year old plate
    #28175279 -

Anyone have success reviving old, dried out cultures?

Discovered a beloved clone of mine in a ketchup cup. It’s completely dried out and has some noticeable dark spots, probably mold. It’s so dehydrated that I had to saw it with my scalpel.



Day 1 after putting to agar and seeing a lot of white dots. Fairly certain it is contam, due to the speed of growth. Some spores probably fell off while I was sawing the dried agar puck.



What are the chances of revival here? Not too optimistic to be honest ahaha

Extras: Filter Print Post Top
Re: Reviving a 2 year old plate [Re: omgflyingbanana]
    #28175291 -

if you still have the original plate i would saw more off and trest it out on water agar and brf pucks


--------------------
Confused? Well now you can!
HHG - cheapest way to start - how i roll

Extras: Filter Print Post Top
Re: Reviving a 2 year old plate [Re: omgflyingbanana]
    #28175667 -

What’s your interest in saving that particular plate. Last of its lineage?


--------------------
----<><><>----

⁂ tail-lights, back-pockets, rearview mirrors ⁂

⁂ guides | grows | supreme air box | sporeaddict ⁂

Extras: Filter Print Post Top
Re: Reviving a 2 year old plate [Re: fiddle_head]
    #28175689 -

Spores are designed to dry out and survive but mycelium will just die when dessicated. I don't see a chance here. Sorry!


--------------------
Nothing in life is to be feared, it is only to be understood.
-Marie Curie


Extras: Filter Print Post Top
Re: Reviving a 2 year old plate [Re: rocky_raccoon] * 2
    #28175693 -

rocky_raccoon said:
Spores are designed to dry out and survive but mycelium will just die when dessicated. I don't see a chance here. Sorry!



Drying out mycelium is actually a well known method for preserving cultures.


--------------------
🍄PANAEOLUS IN MONOTUBS🍄
🍄OFFICIAL SEMILANCEATA THREAD🍄



⭐Baba's Write Ups and Link Collection⭐

Extras: Filter Print Post Top
Re: Reviving a 2 year old plate [Re: Baba Yaga] * 1
    #28175696 -



Works like dream

Extras: Filter Print Post Top
Re: Reviving a 2 year old plate [Re: DERRAYLD]
    #28175700 -

How are you drying yours out?


--------------------
🍄PANAEOLUS IN MONOTUBS🍄
🍄OFFICIAL SEMILANCEATA THREAD🍄



⭐Baba's Write Ups and Link Collection⭐

Extras: Filter Print Post Top
Re: Reviving a 2 year old plate [Re: Baba Yaga] * 2
    #28175702 -

I just leave them in the jar for a few weeks and then place the jar in a sealed bag with desiccant/moisture remover.

I've checked the jar after around 6 months and no mycelium regrew or bounced back after being dried.

Once you transfer individual grains though,  it's maybe 3 days for the mycelium to start growing again.

Extras: Filter Print Post Top
Re: Reviving a 2 year old plate [Re: Baba Yaga]
    #28175730 -

Baba Yaga said:
rocky_raccoon said:
Spores are designed to dry out and survive but mycelium will just die when dessicated. I don't see a chance here. Sorry!



Drying out mycelium is actually a well known method for preserving cultures.


:hewut:


--------------------
----<><><>----

⁂ tail-lights, back-pockets, rearview mirrors ⁂

⁂ guides | grows | supreme air box | sporeaddict ⁂

Extras: Filter Print Post Top
Re: Reviving a 2 year old plate [Re: fiddle_head]
    #28175762 -

looks to me like that myc is waking up and about to jump.
Also looks like those plates are covered in other mold.


--------------------
2023 edible project & species list

Agar to grain/g2g = unlimited mushroomsAMU★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ :coffee:

Extras: Filter Print Post Top
Re: Reviving a 2 year old plate [Re: fiddle_head]
    #28175801 -

bakedbeings said:
if you still have the original plate i would saw more off and trest it out on water agar and brf pucks


I have a bunch of BRF so might give it a go

fiddle_head said:
What’s your interest in saving that particular plate. Last of its lineage?


It is the first clone I ever grew. It came from a single colonized grain that I plucked from a completely contaminated Uncle Ben’s bag inoculated with a shitty syringe from a no-name vendor (newbies, amirite). I cleaned the culture with many plates of potato flake agar and learned the ropes of agar along the way. It produced a high yield but not very potent. However I’d like to revive it for sentimental reasons :smile:

Extras: Filter Print Post Top
Re: Reviving a 2 year old plate [Re: DERRAYLD]
    #28175851 -

DERRAYLD said:
I just leave them in the jar for a few weeks and then place the jar in a sealed bag with desiccant/moisture remover.

I've checked the jar after around 6 months and no mycelium regrew or bounced back after being dried.

Once you transfer individual grains though,  it's maybe 3 days for the mycelium to start growing again.


what do you think about using a heating pad (gasp) to speed things up? things have been gray and rainy in my neck of the woods


--------------------
Confused? Well now you can!
HHG - cheapest way to start - how i roll

Extras: Filter Print Post Top
Re: Reviving a 2 year old plate [Re: bakedbeings]
    #28175858 -

Put the jar in the fridge if you really want to accelerate the drying process.
Fridge will be better than a heat pad imo, just make sure you have solid air exchange.

Extras: Filter Print Post Top
Re: Reviving a 2 year old plate [Re: DERRAYLD]
    #28175859 -

interesting


--------------------
Confused? Well now you can!
HHG - cheapest way to start - how i roll

Extras: Filter Print Post Top
Re: Reviving a 2 year old plate [Re: Baba Yaga]
    #28177840 -

Baba Yaga said:
rocky_raccoon said:
Spores are designed to dry out and survive but mycelium will just die when dessicated. I don't see a chance here. Sorry!



Drying out mycelium is actually a well known method for preserving cultures.


This is absolutely surprising to me! Most organisms just die when they dry out because their inner membranes collapse and their organelles stop functioning. If this really works, cubes must have developed some mechanisms for maintaining their inner cell structures during dry periods. Not uncommon in nature but I wouldn't have expected it in this kind of mushrooms.


--------------------
Nothing in life is to be feared, it is only to be understood.
-Marie Curie


Extras: Filter Print Post Top
Re: Reviving a 2 year old plate [Re: omgflyingbanana]
    #28178152 -

Quote:
However I’d like to revive it for sentimental reasons :smile:


I understand that. I have some B+ plates that are descended from a grow I did 18 months ago. I hope you get some growth. Keep us updated.


--------------------
----<><><>----

⁂ tail-lights, back-pockets, rearview mirrors ⁂

⁂ guides | grows | supreme air box | sporeaddict ⁂

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Great article on how to choose Dessicant DMJ 1,468 8 11/07/03 11:19 AM
by Granola
* I will cast a spell on you all...
( 1 2 3 4 ... 10 11 all )		 AnnoA 28,094 209 03/31/05 03:13 AM
by Anno
* Best desiccant Chamber *DELETED*
( 1 2 3 all )		 12468 9,695 51 04/11/08 04:01 AM
by drftNV
* Could leaving shrooms w/dessicant 3+ days hurt? shroom_assassin 2,609 5 07/23/02 11:59 PM
by cdlove69
* Silica Gel as desiccant? Yarry 3,691 14 11/25/05 12:09 AM
by El_Flying_Mohawk
* dessicator sonicnirvana 1,787 0 10/25/07 09:58 PM
by sonicnirvana
* Problem getting ahold of dessicants.
( 1 2 all )		 TheProdigy 3,522 22 03/24/05 04:37 AM
by ChuangTzu
* Should shrooms touch dessicant shroom_assassin 4,544 10 07/16/02 06:51 PM
by straightbangin

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: DERRAYLD, Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
805 topic views. 16 members, 978 guests and 175 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.007 seconds on 14 queries.