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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Reviving a 2 year old plate
    #28175279 -

Anyone have success reviving old, dried out cultures?

Discovered a beloved clone of mine in a ketchup cup. It’s completely dried out and has some noticeable dark spots, probably mold. It’s so dehydrated that I had to saw it with my scalpel.



Day 1 after putting to agar and seeing a lot of white dots. Fairly certain it is contam, due to the speed of growth. Some spores probably fell off while I was sawing the dried agar puck.



What are the chances of revival here? Not too optimistic to be honest ahaha

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Re: Reviving a 2 year old plate [Re: omgflyingbanana]
    #28175291 -

if you still have the original plate i would saw more off and trest it out on water agar and brf pucks


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Re: Reviving a 2 year old plate [Re: omgflyingbanana]
    #28175667 -

What’s your interest in saving that particular plate. Last of its lineage?


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Re: Reviving a 2 year old plate [Re: fiddle_head]
    #28175689 -

Spores are designed to dry out and survive but mycelium will just die when dessicated. I don't see a chance here. Sorry!


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Nothing in life is to be feared, it is only to be understood.
-Marie Curie


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Re: Reviving a 2 year old plate [Re: rocky_raccoon] * 2
    #28175693 -

rocky_raccoon said:
Spores are designed to dry out and survive but mycelium will just die when dessicated. I don't see a chance here. Sorry!



Drying out mycelium is actually a well known method for preserving cultures.


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Re: Reviving a 2 year old plate [Re: Baba Yaga] * 1
    #28175696 -



Works like dream

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Re: Reviving a 2 year old plate [Re: DERRAYLD]
    #28175700 -

How are you drying yours out?


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Re: Reviving a 2 year old plate [Re: Baba Yaga] * 2
    #28175702 -

I just leave them in the jar for a few weeks and then place the jar in a sealed bag with desiccant/moisture remover.

I've checked the jar after around 6 months and no mycelium regrew or bounced back after being dried.

Once you transfer individual grains though,  it's maybe 3 days for the mycelium to start growing again.

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Re: Reviving a 2 year old plate [Re: Baba Yaga]
    #28175730 -

Baba Yaga said:
rocky_raccoon said:
Spores are designed to dry out and survive but mycelium will just die when dessicated. I don't see a chance here. Sorry!



Drying out mycelium is actually a well known method for preserving cultures.


:hewut:


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Re: Reviving a 2 year old plate [Re: fiddle_head]
    #28175762 -

looks to me like that myc is waking up and about to jump.
Also looks like those plates are covered in other mold.


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Re: Reviving a 2 year old plate [Re: fiddle_head]
    #28175801 -

bakedbeings said:
if you still have the original plate i would saw more off and trest it out on water agar and brf pucks


I have a bunch of BRF so might give it a go

fiddle_head said:
What’s your interest in saving that particular plate. Last of its lineage?


It is the first clone I ever grew. It came from a single colonized grain that I plucked from a completely contaminated Uncle Ben’s bag inoculated with a shitty syringe from a no-name vendor (newbies, amirite). I cleaned the culture with many plates of potato flake agar and learned the ropes of agar along the way. It produced a high yield but not very potent. However I’d like to revive it for sentimental reasons :smile:

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Re: Reviving a 2 year old plate [Re: DERRAYLD]
    #28175851 -

DERRAYLD said:
I just leave them in the jar for a few weeks and then place the jar in a sealed bag with desiccant/moisture remover.

I've checked the jar after around 6 months and no mycelium regrew or bounced back after being dried.

Once you transfer individual grains though,  it's maybe 3 days for the mycelium to start growing again.


what do you think about using a heating pad (gasp) to speed things up? things have been gray and rainy in my neck of the woods


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Confused? Well now you can!
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Re: Reviving a 2 year old plate [Re: bakedbeings]
    #28175858 -

Put the jar in the fridge if you really want to accelerate the drying process.
Fridge will be better than a heat pad imo, just make sure you have solid air exchange.

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Re: Reviving a 2 year old plate [Re: DERRAYLD]
    #28175859 -

interesting


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Confused? Well now you can!
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Re: Reviving a 2 year old plate [Re: Baba Yaga]
    #28177840 -

Baba Yaga said:
rocky_raccoon said:
Spores are designed to dry out and survive but mycelium will just die when dessicated. I don't see a chance here. Sorry!



Drying out mycelium is actually a well known method for preserving cultures.


This is absolutely surprising to me! Most organisms just die when they dry out because their inner membranes collapse and their organelles stop functioning. If this really works, cubes must have developed some mechanisms for maintaining their inner cell structures during dry periods. Not uncommon in nature but I wouldn't have expected it in this kind of mushrooms.


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Nothing in life is to be feared, it is only to be understood.
-Marie Curie


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Re: Reviving a 2 year old plate [Re: omgflyingbanana]
    #28178152 -

Quote:
However I’d like to revive it for sentimental reasons :smile:


I understand that. I have some B+ plates that are descended from a grow I did 18 months ago. I hope you get some growth. Keep us updated.


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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