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Re: The Official Woodlovers Thread [Re: Mr Piggy] * 1
    #28156615 -

Mr Piggy said:
[...]
3) records of a "Jesus" didn't show up until a few hundred years after his "death", so in all likelihood he did not exist as an individual human.  If he didi though homie was definitely a gay man.  Wore robes and sandals while drinking wine all day with 12 dudes and a whore.  It's like every gay bar I've ever been to.


That's hilarious, thanks for that I had a good laugh in the office :rofl2:


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:mushroom2::heart: Growing Semperviva/Subtropicalis - My standard procedure, it all comes down to the basics. :heart::mushroom2:



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Re: The Official Woodlovers Thread [Re: Melgo] * 4
    #28157296 -

I'm basically a sentient intrusive thought.

:babypalm:


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🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: The Official Woodlovers Thread [Re: Mr Piggy] * 9
    #28157755 -



its been warm here i found some azzys from the bed on a walk with ice on the caps


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If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

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Re: The Official Woodlovers Thread [Re: van hatton]
    #28157926 -

:chefskiss:

You and trout should make an iced woodlovers calendar.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: The Official Woodlovers Thread [Re: Mr Piggy]
    #28157964 -

Speaking of azurescens, is this germ plate trying to pin?

Edited by JW123 (01/26/23 12:32 PM)

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Re: The Official Woodlovers Thread [Re: JW123]
    #28158057 -

Azzies dont pin on Agar.


--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

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Re: The Official Woodlovers Thread [Re: Adas]
    #28158129 -

Okay. I didn't think so, but it really looked like it so I had to ask.

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Re: The Official Woodlovers Thread [Re: JW123]
    #28158263 -

Out of curiosity @Adas, could you make it pin if it had sawdust or something else added to the agar? Do you or others here know why it doesnt? Do other woodlovers pin on agar?


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Low Prep the World! (agar plates)
Low Prep the World! (grain prep)
Trashcan FC
Acidulated extraction gummies and drops
Drying agar and grains using parchment paper
:leaf:Cleaning your bubble hash bags


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Re: The Official Woodlovers Thread [Re: JW123] * 1
    #28158280 -

Had 2 jars of cyans which had trich in it.. so I removed the green as much as I could. Jar was already 25% colonized by trich.. nevertheless I decided to do a little experiment.. so I plucked the trich outside of some solid chucks of mycelium (3cm diameter). And added some ripened old chips which were laying there in the garden and had already been mixed with some soil and have been laying there for some months... Just grabbed a few hands of that stuff and added the mycelium chucks (didn't break it up).. was laying in the cold outside open to the elements.. (sheltered from the rain though) 7 days later.. didn't see any trich pop up.. so I moved the bags indoors.. again week later.. still no trich.. decided to add some more spawn now which I had laying around. But yeah.. maybe a way to save partially colonized grain without having to start all over? I wonder if a natural biofilm just fights of trich..

Added more spawn intentionally, just to see what will happen..

Edited by 0t0lerance (01/26/23 02:15 PM)

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Re: The Official Woodlovers Thread [Re: Mr Piggy] * 3
    #28158282 -

Mr Piggy said:
:chefskiss:

You and trout should make an iced woodlovers calendar.


yooooooooo i love mushroom calendars


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

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Re: The Official Woodlovers Thread [Re: YoshiTrainer] * 2
    #28158413 -

YoshiTrainer said:
Out of curiosity @Adas, could you make it pin if it had sawdust or something else added to the agar? Do you or others here know why it doesnt? Do other woodlovers pin on agar?


I mean I guess you could, but you'd also have to keep it for a very long time to starve it, and then introduce the right temps. MAYBE then they could :lol:

I personally have never observed knots in my in vitro experiments ever, but my friend who expands the myc using just woodchips had this happen at least once. It's still a mystery to me what exactly causes knotting. The knots were on the woodchips against the container, not on the surface.


--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

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Re: The Official Woodlovers Thread [Re: Adas]
    #28158444 -

Thank you!


--------------------
Low Prep the World! (agar plates)
Low Prep the World! (grain prep)
Trashcan FC
Acidulated extraction gummies and drops
Drying agar and grains using parchment paper
:leaf:Cleaning your bubble hash bags


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Re: The Official Woodlovers Thread [Re: Adas] * 1
    #28158648 -

Adas said:
I personally have never observed knots in my in vitro experiments ever, but my friend who expands the myc using just woodchips had this happen at least once. It's still a mystery to me what exactly causes knotting. The knots were on the woodchips against the container, not on the surface.


I have a cyanescens plate going that really looks like it's trying to pin (apparent knots, and a couple brown points that look like early pinheads). This plate is also hilariously contaminated and the mycelium appears to be trying to hold off the mold at one end and racing to the edge of the container (as in your friend's case) to fruit at the other. I'll see if I can get a picture, I'll just need to open it somewhere else. FWIW, as mentioned earlier I do use wood soak water in my agar- but I would expect that to mainly be primary decomposition-esque sugars and not lignin. Hell if I know though.

As far as knotting is concerned... I think you actually said it- starving has got to be a factor IMO. It seems they are very sensitive to the availability of nutrition, and when that runs out, and especially when the furthermost strands run into a barrier (guaranteeing no further nutrients along that path), that's when they tend to pop up. Plenty of examples of outdoor wood lovers showing up along edges of garden beds, sidewalks, etc. and I've seen this observation/theory floated a number of times before.

No way can I say this with any authority but if I had to guess, this is part of why cultivated wood lover experiments seem to a) almost invariably take about as long as wild ones to fruit- 9 months or so?- and b) produce so much overlay and often won't fruit at least for a long time in fully colonized chip tubs. Compared to the nutrients available in dune grass roots and partially decomposed hardwood, spawn coming from grain with a concentrated abundance of uncolonized wood is probably pretty fat and happy for a while. The need to reproduce wouldn't become urgent until they've really depleted that supply, which is gonna take a while. (Agar might be different because it's also less nutritious than most cultivated substrates.)

All speculation though.

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Re: The Official Woodlovers Thread [Re: Roostre]
    #28158730 -

Here's the plate in question. Like I said, the situation is p ugly. That divot before the strands pick up again was from me trying to swipe enough for a new plate.



And... enhance!



Enhance again...



It's weird, right? If those aren't knots, my best guess would be that's where the mycelium is overtaking the mold and the discoloration is from metabolites produced in response to that collision. But it doesn't quite look like that to me. And the bigger ones look suspiciously different.

Finally, and I'd be curious to hear from more experienced cultivators on this (so pretty much everyone), that sort of "fan-tail" pattern of growth- a long combined strand that frays and fans out at the end- seems to show up late in the cycle around when knots should or do start to form. Sometimes the exploratory growth on chips does that too, but something about the shape feels different to me.

Time will tell!

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Re: The Official Woodlovers Thread [Re: Roostre] * 1
    #28158736 -

I'm not seeing knots, it appears to be a continuation of the contaminate patches outside of the myc.

But hey, let it grow.  FAFO.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: The Official Woodlovers Thread [Re: Mr Piggy]
    #28158784 -

Mr Piggy said:
But hey, let it grow.  FAFO.


Letters to live by.

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Re: The Official Woodlovers Thread [Re: Roostre]
    #28159261 -

Oh yeah that looks heavily contaminated to me.. when I move too swiftly in the sab or drop something.. bump the tote, I can get a few contaminated spots on my plate. Apart from that your transfers should be clean.. even if you end up exposing receiving jar/plate for a number of seconds and multiple times. but this is quite a bit.. you could start with germination on water agar for T0 next time and then move clean parts to normal agar, but yeah would expand this more agar and see if those stay clean.. if the plate stay clean but contamination rides along try water agar to clean them up, I would wait with moving to grain untill you feel confident. Good luck!

Edited by 0t0lerance (01/27/23 01:57 AM)

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Re: The Official Woodlovers Thread [Re: 0t0lerance]
    #28159360 -

Ya that's not knots. My Azzies turn blue and cream even being completely sterile. It's just a normal aging reaction of the myc, especially on nutritious substrates.


--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

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Re: The Official Woodlovers Thread [Re: Adas]
    #28160094 -

Thanks all for the input!

Fortunately this is just one plate of a few going from that print. It's definitely been a process trying to isolate them out, but I let that one keep going because it seemed to be pushing back against the mold and it's always interesting to see how that plays out on agar. The transfer on that one was before it got nearly that bad so it should be recoverable eventually.

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Re: The Official Woodlovers Thread [Re: Roostre] * 9
    #28160176 -

Woodlovers can be very easy to grow and to maintain in the right area. I received a simple envelope with a handful of colonised Ps cyanescens woodchips, expanded the chips, made some beds, and 20 years later I can still harvest from said patches, mainly due to the fact the gardeners that maintain the area keep adding new woodchips to new places in the area (I did nothing to expand, just spread some of the stembuts and some mycelium in new piles of wood.) I don't know weather the spores from the original beds keep spreading, if it's the mycelium that keeps expanding, or it's purely the great habitat, but I love the fact that I did a little work 20 years ago and still can harvest every autumn around said spots and have the feeling I attributed to that. Plus it's public domain, so anyone who can recognize them, can take a bunch. 






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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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