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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: mushboy]
#27387697 - 07/14/21 05:28 PM (5 years, 2 months ago)

mushboy said:

Attempt?

... what?

Seriously

Jesting aside I’ve been trying to be better about xfering just for the sake of transferring. For less than $200 you can get a mini fridge or even a full size on fb market place and store you extra “backup” plates in.

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D3’s GlogD3's Agar TEKAgar Porn Gallery RW x MelMak Grow Along Thread

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PhosCap
Gratuitous Heavenly Grace

Registered: 06/09/10
Posts: 975
Loc: Tartary
Last seen: 3 years, 4 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27387715 - 07/14/21 05:42 PM (5 years, 2 months ago)

D3monic said:

mushboy said:

Attempt?

... what?

Seriously

Jesting aside I’ve been trying to be better about xfering just for the sake of transferring. For less than $200 you can get a mini fridge or even a full size on fb market place and store you extra “backup” plates in.

Wait a sec. You just leave the swab in the agar plate? I have a PE swab but never started it yet.

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: PhosCap]

1
#27387722 - 07/14/21 05:52 PM (5 years, 2 months ago)

phosi said:

D3monic said:

mushboy said:

Attempt?

... what?

Seriously

Jesting aside I’ve been trying to be better about xfering just for the sake of transferring. For less than $200 you can get a mini fridge or even a full size on fb market place and store you extra “backup” plates in.

Wait a sec. You just leave the swab in the agar plate? I have a PE swab but never started it yet.

Yes n no, depends on how I feel. I streak a couple then break swab and dunk the tip into agar trying to keep the stick off the agar. The wood is more prone to contaminating. You can also use sterile tweezers and pull a little cotton off and set on agar.

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tututotutut
You...! You're not me!

Registered: 01/18/21
Posts: 2,218
Last seen: 5 years, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27387740 - 07/14/21 06:03 PM (5 years, 2 months ago)

My duder Im still struggling just to get less than 100$ for non-bill purchases. Standing in a hole over here! There is no extra agar fridge for me, not for a long time lol. I am pretty confident I must sacrifice them but thanks for the segway back to the first question

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yeah keep telling yourself that's voluntary

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tututotutut
You...! You're not me!

Registered: 01/18/21
Posts: 2,218
Last seen: 5 years, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tututotutut]
#27387742 - 07/14/21 06:04 PM (5 years, 2 months ago)

Quote:

You can also use sterile tweezers and pull a little cotton off and set on agar.

Hahaaa nice i like that. Also have a PE swab been too scared to mess up.
Shucks I suppose you could also just scrape some spores off the swab w cooled sterile scalpel too right?
Last time/first time tried literally swabbing the swab. It didn't go well. Got wrong spores germinated :frown:

:frown:

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yeah keep telling yourself that's voluntary

Edited by tututotutut (07/14/21 06:06 PM)

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Nimpo
Big Black

Registered: 05/10/12
Posts: 2,699

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27387777 - 07/14/21 06:27 PM (5 years, 2 months ago)

D3monic said:

Jesting aside I’ve been trying to be better about xfering just for the sake of transferring. For less than $200 you can get a mini fridge or even a full size on fb market place and store you extra “backup” plates in.

D3 i fucking love those plastic plates man. Im a cheap bitch so im rocking pp5 ketchup cups as plates, but I bought a good amount of those plastic screw ons for cold storage. Have yet to see one contam (i dont wrap them), they hold up well and clarity is solid

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Nimpo]
#27387895 - 07/14/21 07:56 PM (5 years, 2 months ago)

I’m liking them for cold storage as well. A lil small for sitting on the shelf.

Can always send the extra plates to grain, use as masters and then g2g I keep a few pint grain jars around just for this.

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D3’s GlogD3's Agar TEKAgar Porn Gallery RW x MelMak Grow Along Thread

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B H O

Registered: 03/17/13
Posts: 675
Last seen: 2 years, 6 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Nimpo]

1
#27387901 - 07/14/21 08:01 PM (5 years, 2 months ago)

IMO normal petri without the screw on lids would be choice petri. You gotta be quick as fuck and multi tasking both hands. Petris on my left utensil on my right and master in the middle

On a side note,

Got 9 reishi transfers finally pulling through!!

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Edited by B H O (07/14/21 08:03 PM)

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Nichrome
Skank

Registered: 12/17/18
Posts: 8,927

Re: AGAR ENVY! (Anything and All things agar!)

[Re: B H O]
#27388122 - 07/14/21 11:33 PM (5 years, 2 months ago)

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Question everything, it's very responsive.
culture storage

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fahtster
Now With 33%More Faht

Registered: 06/17/06
Posts: 10,866

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Nichrome]
#27388151 - 07/15/21 12:45 AM (5 years, 2 months ago)

Nice.. did I send you that PE pfrs?

Man.. I can hardly wait til the fall :mushroom:

:mushroom:

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Nichrome
Skank

Registered: 12/17/18
Posts: 8,927

Re: AGAR ENVY! (Anything and All things agar!)

[Re: fahtster]
#27388153 - 07/15/21 12:54 AM (5 years, 2 months ago)

You sure did. :hatsoff:

:hatsoff:

Got a nice plate fruit that looked real dickish. It went on it's own plate and then to LC after that.

The plate in todays photo will get at least another transfer or two I suspect, before it starts showing me where to go with it for real. The plate fruit culture was a different, more aloof pattern. I think rhizomorphic and tomentose are too narrow of descriptors sometimes.

--------------------
Question everything, it's very responsive.
culture storage

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fahtster
Now With 33%More Faht

Registered: 06/17/06
Posts: 10,866

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Nichrome]
#27388154 - 07/15/21 12:56 AM (5 years, 2 months ago)

Oooo that’s exciting :popcorn:

:popcorn:

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Nichrome
Skank

Registered: 12/17/18
Posts: 8,927

Re: AGAR ENVY! (Anything and All things agar!)

[Re: fahtster]
#27388160 - 07/15/21 01:11 AM (5 years, 2 months ago)

That PEA+ was from you too right? Got some nice T3-4's going but they're still nowhere close to deciding how they want to organize. I'll just keep going till they do or I get impatient and try some on grain like I often do...

--------------------
Question everything, it's very responsive.
culture storage

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fahtster
Now With 33%More Faht

Registered: 06/17/06
Posts: 10,866

Re: AGAR ENVY! (Anything and All things agar!)

1
#27388171 - 07/15/21 01:24 AM (5 years, 2 months ago)

Yessir

:grin:

excited to see what ya get. Not to thread Jack too much but this is how my PE/pfrs f2 turned out (only did a microgrow so far). got both phenos but no red spores

Faht

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SpaceBaby
alchemist

Registered: 11/01/20
Posts: 2,030
Loc: SPACE
Last seen: 3 years, 13 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: fahtster]
#27388224 - 07/15/21 03:55 AM (5 years, 2 months ago)

Morning madness.

2000 ml agar poured to petris before 5:00 AM

Got a major transfer orgy imminent.

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SpaceBaby
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aNewBeginning

Registered: 06/26/21
Posts: 708

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27388247 - 07/15/21 04:32 AM (5 years, 2 months ago)

Quote:

D3monic said:Jesting aside I’ve been trying to be better about xfering just for the sake of transferring.

I'm just over a month and a half into this hobby and am already doing this.

It's great fun. I'm about to do T4 xfers which started with a mazatapec print.
I've already put 6 plates to grain.
At this stage, the plates are now purely rhizomorphic.

There isn't really any point in putting them to grain because who knows if they'll fruit.

Despite knowing all of this, I'm doing more xfers in a few hours.

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aNewBeginning

Registered: 06/26/21
Posts: 708

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Nichrome]
#27388252 - 07/15/21 04:48 AM (5 years, 2 months ago)

Nichrome said:

I think rhizomorphic and tomentose are too narrow of descriptors sometimes.

This is a great point.

In "The Mushoom Cultivator", Paul Stamets says

Quote:

Another aspect of the mycelial appearance is its type of
growth, whether it is aerial or appressed, cottony or rhizomorphic.

He also mentions a form of mycelium called "linear mycelium" which grows "longitudinally radial" and does not have "twisted strands of interwoven hyphae that characterize the rhizomorphic kind." He labels this type of mycelium as "intermediate".

Could we perhaps view tomentose, intermediate and rhizomorphic on a spectrum/continuum?

The myceliums position on the continuum can change due to various "natural" factors (environment, humidity, temperature) as well as "artficial" factors (transfers).

Maybe it's not even important to label the appearance of mycelium but after observing multiple forms of "rhizomporphic" mycelium it really makes you think.

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A.k.a
Stranger

Registered: 10/27/19
Posts: 18,220
Loc: Gaming the system
Last seen: 22 hours, 24 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: aNewBeginning]
#27388303 - 07/15/21 06:30 AM (5 years, 2 months ago)

The intermediate sounds like what we were talking about in another thread recently.

I always just thought of it as well organized tomentose growth but intermediate is a decent description.

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aNewBeginning

Registered: 06/26/21
Posts: 708

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#27388324 - 07/15/21 07:25 AM (5 years, 2 months ago)

A.k.a said:

The intermediate sounds like what we were talking about in another thread recently.

I always just thought of it as well organized tomentose growth but intermediate is a decent description.

It helps classify it, I guess.

Stamets also claims

Quote:

Linear mycelium canalso produce
abundant primordia but this usually occurs soon after it forms rhizomorphs.

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A.k.a
Stranger

Registered: 10/27/19
Posts: 18,220
Loc: Gaming the system
Last seen: 22 hours, 24 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: aNewBeginning]
#27388331 - 07/15/21 07:32 AM (5 years, 2 months ago)

Yeah I think all myc forms rhizo veins when it fruits.

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