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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27334992 -

That LC test plate is A rainbow farmin wet dream. Nice


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: AGAR ENVY! (Anything and All things agar!) [Re: verum subsequentis]
    #27335533 -

Okay Masters of Agar, I was up late last night reading Mad Season's thread about hidden contamination on agar, I've been having trouble with this GT progression and I believe I see the mycelium "lifting" on this plate. Am I correct?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: xVandalx]
    #27336136 -

xVandalx said:
Mad Season's thread about hidden contamination on agar


Sorry I have no info for you but could you link to this thread please?? I've been dying to learn more on this subject :takingnotes:


This TAT ms is going crazy, as is the T1. Throwing ropes!



Bonus: my first LC growing... something :shrug: Fingers crossed



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Re: AGAR ENVY! (Anything and All things agar!) [Re: Benson]
    #27336139 -

Here you go my friend:
Hidden contams

          :cookiemonster:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Goatrider]
    #27336153 -

Goatrider said:
Here you go my friend:
Hidden contams

          :cookiemonster:


My guy :rockon::cheers:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Benson]
    #27336192 -



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D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Benson]
    #27336259 -

Benson said:
xVandalx said:
Mad Season's thread about hidden contamination on agar


Sorry I have no info for you but could you link to this thread please?? I've been dying to learn more on this subject :takingnotes:


This TAT ms is going crazy, as is the T1. Throwing ropes!



Bonus: my first LC growing... something :shrug: Fingers crossed




Crossing them with you, Benson.

I have multiple firsts going. Like the whole house is turning into a lab/grow room.:rofl:

I look forward to developments.


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SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SpaceBaby]
    #27336341 -

I've just gotten in to agar recently and it really is a bunch of fun!

I'm having some troubles with contams the last two times I've transferred and was wondering where I could be messing up. The last two times I've done transfers, every plate except one or maybe two has contams that all look the same. Could it be from the plate that I'm transferring from even though it looks okay? Other than that I was thinking I'm not preparing the SAB properly.. Sorry the lighting in the pics is bad, but I hope it's clear enough.



There is one plate that's looking promising though, so that's exciting!


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Re: AGAR ENVY! (Anything and All things agar!) [Re: glive] * 1
    #27336346 -

Your sterile technique looks off and it also looks like you've gotten something riding along with your transfers as well. Tighten up your technique working on your SAB


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Crack's Tek's and Shit


Edited by Crackatoa (06/05/21 03:56 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Crackatoa]
    #27336792 -



crack what recipe are u using for them plates bro?

Edited by Culliganman360 (06/05/21 11:25 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Crackatoa]
    #27336836 -

Crackatoa said:
Your sterile technique looks off and it also looks like you've gotten something riding along with your transfers as well. Tighten up your technique working on your SAB



Beautiful crack !!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Culliganman360] * 1
    #27336839 -

Culliganman360 said:


crack what recipe are u using for them plates bro?


600ml water
10g agar
7g LME
1g yeast

Thank you Sam


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Crack's Tek's and Shit


Edited by Crackatoa (06/06/21 12:40 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Crackatoa]
    #27336995 -

Crackatoa said:
Your sterile technique looks off and it also looks like you've gotten something riding along with your transfers as well. Tighten up your technique working on your SAB



Thanks a lot for the tips! I got some new blades, took some more care and transferred from what looks like a clean plate. We'll see what happens!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: TheDoobsker]
    #27337030 -

TheDoobsker said:
Yeah I just use a metal straw and get it red hot. I first saw D3monic do it but I think lots of others on this thread also use the method.

I should add, I only transfer this way once my culture is already clean. If I’m isolating away from contams, I cut a square out with my scalpel so I can get a much smaller piece of agar transferred.


how do you get the transfer out of the straw? does it not stick inside?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: greenladel]
    #27337035 -

Sometimes it sticks but I find that happens if you penetrate the agar all the way to the dish, if you only lightly mark it and use the scalpel to cut/ lift out off of the agar it's easier.

Edited by DERRAYLD (06/06/21 06:12 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27337038 -

DERRAYLD said:
Sometimes it sticks but I find that happens if you penetrate the agar all the way to the dish, if you only lightly mark it and use the scalpel to cut/ lift out off of the agar it's easier.


ah okay that makes sense, thanks.
i might try to do something with another metal rod through the middle of a metal straw to poke it out.

thanks for the tips

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Re: AGAR ENVY! (Anything and All things agar!) [Re: greenladel]
    #27337041 -

There are guys that have purchased or made biopsy tools like that with an internal rod to push the slug out.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27337309 -

Out of several thousand transfers I’ve had them stick maybe 3-4 times. Usually if tool too hot or if your tool has a bit of wall thickness.


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D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Crackatoa]
    #27337359 -

Crackatoa said:

600ml water
10g agar
7g LME
1g yeast


Crackatoa, what's the name of the yeast you use?


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Granny's Awesome LAGM21

>Things I wish I knew when I started<

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Threads2theSoul]
    #27337369 -

I use bragg's right now. I'm going to order the kind demonic uses next amazon order though.


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Crack's Tek's and Shit


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