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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: PitcherCrab]
    #27332184 -

I’ve been sending transfers to both lma and the mya. Some really like it. Both my enigma and ape where light and whispy on lme but thickened up and started to go rhizo on the yeast.


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D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc] * 3
    #27332324 -

AA+ on slant after a year in fridge.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Crackatoa]
    #27332918 -

Crackatoa said:
Yeah, I've dropped mine pretty low. I'll go to 7-8g per 600ml. I'll do 10g agar, 7-8g lme and 1g yeast for 600ml.


Crackatoa said:
Crack, why? Always curious. Results?

If this has been answered, sorry. It's been unposted on my phone for a while.


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SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SpaceBaby]
    #27332981 -

Why I go low on nutrients? Faster growth. Less nutes the quicker it moves for food. Too much and the myc will stay and eat in one spot. It's more technical than that but :shrug:


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Crack's Tek's and Shit


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Crackatoa]
    #27333018 -

my melmak transfer 1s as of today.





i'm doing some tests with low nutes vs. higher nutes on all my transfers to see how they react, so if you'd like to see what lower/higher nutes get you (at least with my plates) follow this thread where ill update as i go :  https://www.shroomery.org/forums/showflat.php/Number/27325787

i was having issues and inconsistencies and was given advice to go higher nutrition, but also given advice to go lower, so only one way to find out.

Edited by moshe1 (06/02/21 08:45 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Crackatoa]
    #27333168 -

Crackatoa said:
Why I go low on nutrients? Faster growth. Less nutes the quicker it moves for food. Too much and the myc will stay and eat in one spot. It's more technical than that but :shrug:


This topic is really interesting to me. For me, adding nutritional yeast is what gets my cultures growing fast and also rhizo. They’re like 50% faster growth with yeast compared to no yeast.

I sent some cultures to different nute levels months ago and results for me kinda looked like fastest growth at 20g lme. Not very conclusive though. It’s also crazy to me how strongly they lost their rhizomorphic growth with no yeast on the media.



These all came from agar media that had bragg’s yeast.



I wanted to send them all back to a plate with yeast and see if they went back rhizo, but it was timed perfectly with me needing to tear down my grow room and put cult on pause for a couple months while I find a new place. I regret not setting up a positive control with my typical recipe which includes yeast. Definitely planning to setup a more extensive experiment on agar recipes in the future.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: TheDoobsker]
    #27333213 -

you are the third person to mention in discussion that yeast is making all the difference. wonder why that is


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Edited by moshe1 (06/03/21 12:47 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: moshe1]
    #27333233 -

I recently started adding yeast as well and have seen decent results. I'd like to make a control and test certain recipes against it, but need to pour more and narrow down a culture.


Still not sure what to make of the monster that snuck into my agar bottle.




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Re: AGAR ENVY! (Anything and All things agar!) [Re: TheDoobsker]
    #27333234 -

TheDoobsker said:
Crackatoa said:
Why I go low on nutrients? Faster growth. Less nutes the quicker it moves for food. Too much and the myc will stay and eat in one spot. It's more technical than that but :shrug:


This topic is really interesting to me. For me, adding nutritional yeast is what gets my cultures growing fast and also rhizo. They’re like 50% faster growth with yeast compared to no yeast.

I sent some cultures to different nute levels months ago and results for me kinda looked like fastest growth at 20g lme. Not very conclusive though. It’s also crazy to me how strongly they lost their rhizomorphic growth with no yeast on the media.



These all came from agar media that had bragg’s yeast.



I wanted to send them all back to a plate with yeast and see if they went back rhizo, but it was timed perfectly with me needing to tear down my grow room and put cult on pause for a couple months while I find a new place. I regret not setting up a positive control with my typical recipe which includes yeast. Definitely planning to setup a more extensive experiment on agar recipes in the future.


Nice plates! I’m sold, I’ll be adding yeast back into all my agar after all these beautiful yeasty plates.


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PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear


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Re: AGAR ENVY! (Anything and All things agar!) [Re: PitcherCrab]
    #27333304 -

Thanks, pitcher! Forgot to mention that the above experiment recipes were transferred from these exact yeast plates I have posted, if that wasn’t clear.

______________________________________________________
Gandalf, my guess is some other type of mold that you got there. Not magic mycelium is my guess. But yeah pouring a bunch of different recipes to test them is way more work than it sounds like lol

And then transferring 40 plates at once in a 66qt SAB was the real challenge



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Re: AGAR ENVY! (Anything and All things agar!) [Re: TheDoobsker]
    #27333339 -

I still find no need for yeast. Never tried it because I haven't found a reason to. Might give her a whirl just for fun.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: AGAR ENVY! (Anything and All things agar!) [Re: verum subsequentis] * 1
    #27333348 -

My cultures looked terrible on LMEA. Tried upping nutes, lowering nutes, nothing. Just really blotchy ugly cultures.

Added yeast, perfect plates every time since.

I was going crazy seeing all these pictures of nice plates and finding myself unable to produce similar results with the same recipe.

I know nogo and others have expressed similar feelings.

Aesthetics aren't all that important but for me, it's always easier to transfer from plates that have very clean pronounced growth.



Homestead PE. Didn't poop in this just for you Bugatti Verum

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mr. Mushie]
    #27333351 -

Thanks. I really appreciate that.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: AGAR ENVY! (Anything and All things agar!) [Re: TheDoobsker]
    #27334144 -

TheDoobsker said:
Crackatoa said:
Why I go low on nutrients? Faster growth. Less nutes the quicker it moves for food. Too much and the myc will stay and eat in one spot. It's more technical than that but :shrug:


This topic is really interesting to me. For me, adding nutritional yeast is what gets my cultures growing fast and also rhizo. They’re like 50% faster growth with yeast compared to no yeast.

I sent some cultures to different nute levels months ago and results for me kinda looked like fastest growth at 20g lme. Not very conclusive though. It’s also crazy to me how strongly they lost their rhizomorphic growth with no yeast on the media.



These all came from agar media that had bragg’s yeast.



I wanted to send them all back to a plate with yeast and see if they went back rhizo, but it was timed perfectly with me needing to tear down my grow room and put cult on pause for a couple months while I find a new place. I regret not setting up a positive control with my typical recipe which includes yeast. Definitely planning to setup a more extensive experiment on agar recipes in the future.


May I ask how you take your tissue/agar for transfers? Those look perfectly round... looks like a punch tool of some kind.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: xVandalx]
    #27334216 -

Yeah I just use a metal straw and get it red hot. I first saw D3monic do it but I think lots of others on this thread also use the method.

I should add, I only transfer this way once my culture is already clean. If I’m isolating away from contams, I cut a square out with my scalpel so I can get a much smaller piece of agar transferred.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: TheDoobsker]
    #27334217 -

:whathesaid:


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Crack's Tek's and Shit


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Crackatoa]
    #27334285 -


Natalensis


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Never give up, never surrender.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Failboat]
    #27334775 -



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All I need are some tasty waves, a cool buzz, and I’m fine.
Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Crackatoa]
    #27334813 -

I find the opposite to this. If I don’t put yeast and peptone in the mix the culture grows like shit. I never have any issue with colonizing a plate in 10 days.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nogoodnamesleft]
    #27334827 -

Too much condensation but it's growing nicely.

Burma from a hopelessly dirty syringe that I have been isolating to clean.



First plate from the shitty lc






Edited by DERRAYLD (06/04/21 08:52 AM)

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