Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

Sporeworks
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Autoflowering Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate

Jump to first unread post Pages: 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Help me on my First grow. Spores to agar to grain to monotubs
    #26791232 -

I made agar in tequila bottles at 15gr agar, 12 gr MLE and 750 ml water. I mixed those ingredients in a saucepan and brought to a boil. as soon as it came to boil you pour it into your bottles then pressure cooked at 15 PSI for 30 min. then used my sterilizer as a 120-130F waterbath.


I then poured plates and set hot water on top. The hot water did nothing. I let them sit for 24 hours with no parafilm in stacks and the condensation went away. However, before i used them in my SAB I sprayed with ISO and got condensation on some. Next time i will not spray my stacks with ISO before putting into SAB. I wiped each plate down and dried so it shouldn't be a problem.


I smoked some weed (legal state)


These are the leftover plates that will get wrapped and put into fridge


I used my SAB to inoculate at least 10 plates of:
Amazon pes
Hawaiian PES
Red Boys
Ecuadorian

Okay i still find it hard to do just one drop on each dish AND I CAN BARELY SEE IN MY SAB TRYING TO DO TWO SINGLE DROPS. am I the only one who is like "holy shit, this isnt easy!" I have to hunch down to see through my arm hole and try very careful not to squeeze too hard and im successful, but sometimes I get a squirt and I just opened another dish and tried to pour the extra into the other dish. Probably gonna contam, but fuck it. I also got two different syringes from this source and one was way easier than the other. Oh well. I try so hard to do a single drop haha.

Plates at 77.










All I will do over next few days is maybe remove parafilm to rid condensation, especially if it isn't gone by tomorrow I will carefully remove all parafilm and allow dishes to do their thing until moisture is gone then I'll replace parafilm

Edited by Forevergreen (07/19/20 03:13 PM)

Extras: Filter Print Post Top
Re: First grow. Spores to agar to grain to monotubs [Re: Forevergreen]
    #26791258 -

Good job mate! Looking good. Visibility in a SAB can be a pain, I'd recommend getting a clamp lamp or LED shop light for sterile work. Game changer. I also used to elevate the entire table my
SAB was on with 8" plastic bed risers which put it at around shoulder level while sitting. This allowed me to just stick my arms straight out with elbows sometimes resting on the edge of the table to work and do a lot less hunching. Good luck, and look forward to future progress!

Edited by Rapjack (06/29/20 02:44 AM)

Extras: Filter Print Post Top
Re: First grow. Spores to agar to grain to monotubs [Re: Rapjack]
    #26791340 -

just a lil trick i use to get rid of condensation on tops of my plates. il use  a very very light heat source just above the lids of the plates.


--------------------
PANAEOLUS FRUITING MADE SIMPLE


Extras: Filter Print Post Top
Re: First grow. Spores to agar to grain to monotubs [Re: jcm4620]
    #26791465 -

Just leaving them in stacks usually works for me. I’ll put an old plate or two up top and just let them take all the condensation.

Also you don’t need to store plates in the fridge, it’s actually better to leave them at room temp so that anything nasty shows itself before you go using that plate.

Spore prints are much easier to work with in my experience than spore syringes. I hate syringes. I’m hoping you get clean growth right out and you go on to grow many trippy mushrooms. But if you keep getting plates full of bacteria don’t get discouraged, just get a print. Starting with agar is the way to go though :thumbup:


--------------------
Sour grapes, sweet revenge
Heaven starts right where hell ends

Extras: Filter Print Post Top
Re: First grow. Spores to agar to grain to monotubs [Re: Roger Clemency]
    #26791630 -

ya prints are way better 😃 il see if i have any cubes left when i get home from work tonite. if i do il send ya one. im just not sure if i have any cube prints left. i think all i have right now is some pan alabamas but il check for u. and if i dont im sure someone will be kind enough to send ya one


--------------------
PANAEOLUS FRUITING MADE SIMPLE


Extras: Filter Print Post Top
Re: First grow. Spores to agar to grain to monotubs [Re: jcm4620]
    #26791642 -

You can also germinate your syringe on some grain, then pluck a few individual grains out of the jar and put them on agar to get the culture cleaned up.

Pretty sure it was jcm who gave me the idea originally. Works great, I hate squirting a syringe onto an agar plate.


--------------------
:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:
:mushroom:                  :mushroom2:                :mushroom:
:mushroom:            :mushroom2::mushroom2::mushroom2:            :mushroom:
:mushroom:      :mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:      :mushroom:
:mushroom::cookiemonster: BEHOLD :elmo::mushroom:
:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:

Extras: Filter Print Post Top
Re: First grow. Spores to agar to grain to monotubs [Re: metaphoric]
    #26791661 -

more and more people are saying this... I'd like to know what the super-specialists think


--------------------

Extras: Filter Print Post Top
Re: First grow. Spores to agar to grain to monotubs [Re: SawCeeJack]
    #26791689 -

ya i do that if i have a hard time with germ on agar 😃


--------------------
PANAEOLUS FRUITING MADE SIMPLE


Extras: Filter Print Post Top
Re: First grow. Spores to agar to grain to monotubs [Re: SawCeeJack]
    #26791708 -

AtmozFear said:
more and more people are saying this... I'd like to know what the super-specialists think



Disclaimer - all of the following have been forged. For entertainment purposes only.

Pastywhyte said:
Spore syringes make you gay. More so than just eating or growing mushrooms alone:


cronicr said:
Using spore syringes basically supports terrorism. How many middle eastern girls do you want to be maimed by your mush cult choices??


fahtster said:
Never use syringes to agar. Firstly it makes you irreversibly gay, and secondly it will lead to extremely low potency mushrooms...if they ever even germinate


AtmozFear said:
Roger Clemency is a d!ck and keeps misquoting people, sowing chaos and confusion



--------------------
Sour grapes, sweet revenge
Heaven starts right where hell ends

Extras: Filter Print Post Top
Re: First grow. Spores to agar to grain to monotubs [Re: Roger Clemency]
    #26791718 -

They're all talking about spawning the grain you noc up with a syringe. That's not what we're talking about.

I'm going to ignore the fact that they are fake quotes and address the point I think you're trying to make.


--------------------
:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:
:mushroom:                  :mushroom2:                :mushroom:
:mushroom:            :mushroom2::mushroom2::mushroom2:            :mushroom:
:mushroom:      :mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:      :mushroom:
:mushroom::cookiemonster: BEHOLD :elmo::mushroom:
:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:

Extras: Filter Print Post Top
Re: First grow. Spores to agar to grain to monotubs [Re: jcm4620]
    #26791758 -

jcm4620 said:
ya prints are way better 😃 il see if i have any cubes left when i get home from work tonite. if i do il send ya one. im just not sure if i have any cube prints left. i think all i have right now is some pan alabamas but il check for u. and if i dont im sure someone will be kind enough to send ya one


Wow that would be really cool. Not expecting anything here other than y'alls help.

I'm going to noc up these wide mouth pint jars, I just bought WBS with a bunch of cracked corn and my dumbass didn't notice til I got home because it didn't list it on the ingredients.. Im just gonna buy another bag and buy a bird feeder or something and use the old bag for the birds. I don't save receipts and take it as a lesson. Birds get fed now lol

The tractor supply store also sells oats for $15.. Debating doing just WBS or half jars WBS and other half Oats.


--------------------
My first grow:
https://www.shroomery.org/forums/showflat.php/Number/26791232

Edited by Forevergreen (06/29/20 10:06 AM)

Extras: Filter Print Post Top
Re: First grow. Spores to agar to grain to monotubs [Re: Forevergreen]
    #26791767 -

Wbs has more inoculation points but oats are basically fool-proof. I love oats, got started with wbs first.


--------------------
:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:
:mushroom:                  :mushroom2:                :mushroom:
:mushroom:            :mushroom2::mushroom2::mushroom2:            :mushroom:
:mushroom:      :mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:      :mushroom:
:mushroom::cookiemonster: BEHOLD :elmo::mushroom:
:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:

Extras: Filter Print Post Top
Re: First grow. Spores to agar to grain to monotubs [Re: Roger Clemency]
    #26791770 -

your entertainment doesn't make any sense  :confused:


--------------------

Edited by SawCeeJack (06/29/20 10:11 AM)

Extras: Filter Print Post Top
Re: First grow. Spores to agar to grain to monotubs [Re: SawCeeJack]
    #26791795 -

It was more about not using syringes to agar then about germinating on grain and then going to agar for trouble syringes, which I guess is what you meant.

The OP is probably like wtf is going on I haven’t even had a problem yet. I’m just here trying to hate on syringes bro, don’t try to confound me.


--------------------
Sour grapes, sweet revenge
Heaven starts right where hell ends

Extras: Filter Print Post Top
Re: First grow. Spores to agar to grain to monotubs [Re: Roger Clemency]
    #26791799 -

like i said would love to hear the experts on this... :undecided:


--------------------

Extras: Filter Print Post Top
Re: First grow. Spores to agar to grain to monotubs [Re: SawCeeJack]
    #26791886 -

Lol about what though?


--------------------
Sour grapes, sweet revenge
Heaven starts right where hell ends

Extras: Filter Print Post Top
Re: First grow. Spores to agar to grain to monotubs [Re: Forevergreen]
    #26791962 -

Roger Clemency said:
Disclaimer - all of the following have been forged. For entertainment purposes only.


Homophobia isn’t terribly entertaining.


Forevergreen said:
am I the only one who is like "holy shit, this isnt easy!"


Someone please correct me if this is awful advice, but if you squirt a little on a piece of clean aluminum foil rather than directly onto the agar, you could wet your loop in the foil puddle and then put it on the agar.

Extras: Filter Print Post Top
Re: First grow. Spores to agar to grain to monotubs [Re: Roger Clemency]
    #26791993 -

Roger Clemency said:
Lol about what though?


MS2G

G2A

A2?

Extras: Filter Print Post Top
Re: First grow. Spores to agar to grain to monotubs [Re: Capsicum Chinense]
    #26792023 -

As a true freak I suffer from no obias Phrontist :smile:

Agar back to grain (or LC or LI) to finish that thought, only now the myc has been separated from the bacteria that was living all in the syringe water, making it unfeasible to go MSS to agar in the first place.


--------------------
Sour grapes, sweet revenge
Heaven starts right where hell ends

Extras: Filter Print Post Top
Re: First grow. Spores to agar to grain to monotubs [Re: Roger Clemency]
    #26792054 -

heres a good example why syringes suck big floppy donkey balls



--------------------
PANAEOLUS FRUITING MADE SIMPLE


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |  [ show all ]

Shop: Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Autoflowering Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Re: Any help to grow P. Azurenscens??? spores 1,222 4 01/12/00 03:40 PM
by spores
* Why grow on agar? stcore 2,098 1 07/29/02 05:03 PM
by bluhoney
* How long do spores take to grow on whole grain? Maxawow 1,932 2 06/14/01 10:19 PM
by Maxawow
* grain transfers and spore printing esin 1,377 1 05/22/02 06:12 AM
by angryshroom
* Agar and growing that white stuff! pj541 2,795 5 10/15/01 04:06 PM
by pj541
* Re: Use agar and break free from spore vendor dependency forever for only $10!
( 1 2 3 all )		 Anonymous 7,988 41 02/04/01 12:43 PM
by puscle
* The ease of rye grain. Anonymous 15,360 19 06/03/16 03:10 PM
by Disco Shiny
* Re: Growing shrooms on a bioreactor paddoholland 2,206 13 03/20/01 11:45 PM
by paddoholland

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
4,800 topic views. 14 members, 832 guests and 114 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.008 seconds on 16 queries.