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jcm4620
Stranger


Registered: 05/26/19
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Re: First grow. Spores to agar to grain to monotubs [Re: Forevergreen]
#26825881 - 07/15/20 07:50 PM (3 years, 6 months ago) |
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stay off redit those ppl are almost all full of shit bro
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Forevergreen
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Re: First grow. Spores to agar to grain to monotubs [Re: jcm4620]
#26825925 - 07/15/20 08:07 PM (3 years, 6 months ago) |
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Well thank god for my homies on shroomery. Im using those GT prints tonight.
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F. 3
Flowing

Registered: 04/29/17
Posts: 192
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Re: First grow. Spores to agar to grain to monotubs [Re: jcm4620]
#26826269 - 07/16/20 02:45 AM (3 years, 6 months ago) |
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Quote:
jcm4620 said: stay off redit those ppl are almost all full of shit bro
take this advice
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jcm4620
Stranger


Registered: 05/26/19
Posts: 6,700
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Re: First grow. Spores to agar to grain to monotubs [Re: F. 3]
#26826314 - 07/16/20 03:52 AM (3 years, 6 months ago) |
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sweet if u have bad luck with my prints and ghe stwrile swab packets i sent u i would seeiously review your sterile technique. good luck man and be sure to post your results 😃😃
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Roger Clemency
Smile


Registered: 03/23/20
Posts: 2,005
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Re: First grow. Spores to agar to grain to monotubs [Re: jcm4620]
#26826402 - 07/16/20 06:55 AM (3 years, 6 months ago) |
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I was referring to the T1 H last plate pic. If I’m not sure about a plate of my own in real life, then it’s mold. If I have to wonder and question I just won’t use it unless it’s strictly out of curiosity but if I need to succeed I look for cube myc.
That H plate is cube myc. Anything else that doesn’t look like that or similar just stay away from for a while. I wouldn’t trust other people’s opinions (especially not mine 😂) over the interweb. You can see these things up close and personal and there are a ton of pics on here to compare. With a standard agar recipe of LMEA you should be able to tell what’s what in most cases.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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Forevergreen
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Re: First grow. Spores to agar to grain to monotubs [Re: jcm4620]
#26827955 - 07/16/20 10:38 PM (3 years, 6 months ago) |
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Quote:
jcm4620 said: sweet if u have bad luck with my prints and ghe stwrile swab packets i sent u i would seeiously review your sterile technique. good luck man and be sure to post your results 😃😃
I saved the swabs for later and just used my flame sterilized scalpel. I like scraping the spores right into the middle of the dish, and it allows me to scrape almost all the spores off the foil. Im feeling confident in my sterile technique as of now. Im not stacking 20 plates at a time in the box for transferring or inoculating like I used to. I would still respray but it was just too much at once. I do 5 dishes and respray the SAB, Respray my hands everytime they leave the box etc. Repeat. Young padawon is learning. Ohmmmmmmmm
Also for some reason (drugs or my mutt human genes) when I smell myc it smells slightly minty until I smash it and then it smells like straight mushrooms so im going to stop assuming so much of my myc is trich based on smell... If its pure white then its myc.. I know thats not 100% true but its what im going by.
I have some dishes i nocd with a print and the ONLY white stuff growing is from the spores.
Edited by Forevergreen (07/16/20 10:55 PM)
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Forevergreen
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Re: First grow. Spores to agar to grain to monotubs [Re: Forevergreen]
#26827971 - 07/16/20 10:52 PM (3 years, 6 months ago) |
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All my plates about to get wrapped. Theres spore prints and transfers. This is after the SAB has been broken down and everything wiped down again before starting parafilm. SAB session took an hour or so. Grabbed a beer and took this pic.
Back to work! Time to parafilm all of these.
Strains I have of actual myc: In jars: amazonian and Hawaiian. I think there is one ecuador and red boy jar that shows mycelium but not too hopeful on those.
Only 1 amazon transfer dish and its only for shits and giggles. Tons of Hawaiian myc transfers. Gulf Coast spore dishes transferred to new plates. Any spore sections with fuzz I transferred to new dish.
I inoculated Golden Teacher, Blue meanie (cubes), and cambodian from spore prints to dishes.
Love yall
Edited by Forevergreen (07/16/20 11:00 PM)
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F. 3
Flowing

Registered: 04/29/17
Posts: 192
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Re: First grow. Spores to agar to grain to monotubs [Re: Forevergreen]
#26828164 - 07/17/20 03:54 AM (3 years, 6 months ago) |
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You should wrap them inside of your sab brother.
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Forevergreen
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Re: First grow. Spores to agar to grain to monotubs [Re: F. 3]
#26829018 - 07/17/20 01:29 PM (3 years, 6 months ago) |
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I do spray em down with ISO and soap mix then let dry before I wrap but okay I will from now on.
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Forevergreen
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Re: First grow. Spores to agar to grain to monotubs [Re: Forevergreen]
#26831850 - 07/19/20 03:07 AM (3 years, 6 months ago) |
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I want to take a moment to thank everyone for their time and helping me get this far. Anyways on with the show.
Clean hawaiian plates T2 (T0 being inoculation)
Gulf Coast T1 transfers. On a plate you will notice an X. Thats a contam. Do i let the myc grow a little or just transfer whats in there to a new dish asap?
Hawaiian MS to grain jars. This guy had moisture in the jar and had beautiful white mycelium. He was shook a few days ago and before I took this picture. I continued to break it apart more after. It is ready for a g2g in a few days or I can spawn it to a shoebox. I vote g2g.
And how do these last two look? They also got shook a few days ago. I dont think its cobweb. They are all hawaiian except the last pic the bottom jars have amazon and red boy. Enjoy fellas.
Edited by Forevergreen (07/19/20 10:47 AM)
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Forevergreen
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Re: First grow. Spores to agar to grain to monotubs [Re: Forevergreen]
#26833310 - 07/19/20 09:47 PM (3 years, 6 months ago) |
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Well i didnt know youre not supposed to shake jars past 30%... I just shook all my jars especially the colonized ones and may have fucked them up.. The one that looked really good jar 4, its all wet with moisture and not recolonizing so I fucked that jar up. Wish i knew that before i shook them all... Oh well...
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TheGreatGig
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Re: First grow. Spores to agar to grain to monotubs [Re: Forevergreen]
#26833534 - 07/20/20 02:48 AM (3 years, 6 months ago) |
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Generally people say not to shake more than once to not damage the mycelium, but 2 shakes before final shake to spawn isn't as bad as people make it out to be. If it didn't recover you probably had an unknown contam or bacteria in those jars before the shake.
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F. 3
Flowing

Registered: 04/29/17
Posts: 192
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Re: First grow. Spores to agar to grain to monotubs [Re: TheGreatGig]
#26833556 - 07/20/20 03:15 AM (3 years, 6 months ago) |
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Remember you are dealing with a living organism, treat it as a child  you probably can do many shakes but its just not worth it. it will take more energy and might stall the growth too. grain looks good tho, judging the jars from one side through a screen 
nonetheless  keep it up man you are doing good.
about the contaminated plates, I would do it asap if you really want the mycelium from those plates. if you got decent uncontaminated plates of the same kind I would ditch the work with contams. if no proceed with steady hands. good luck
Edited by F. 3 (07/20/20 03:18 AM)
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Phrontist
Determined



Registered: 07/17/13
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Re: First grow. Spores to agar to grain to monotubs [Re: Forevergreen]
#26833985 - 07/20/20 10:27 AM (3 years, 6 months ago) |
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Quote:
Forevergreen said: Well i didnt know youre not supposed to shake jars past 30%
This is about preference, it’s not a rule. I like to have 50-70% colonization before shaking and it has yet to cause a problem.
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Forevergreen
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Re: First grow. Spores to agar to grain to monotubs [Re: F. 3]
#26834421 - 07/20/20 02:49 PM (3 years, 6 months ago) |
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Okay I will let the jars try to recover before I g2g. And why not try to get practice getting myc away from contams. The more clean plates, the merrier.
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metaphoric
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Registered: 05/18/15
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Re: First grow. Spores to agar to grain to monotubs [Re: Forevergreen]
#26834520 - 07/20/20 03:43 PM (3 years, 6 months ago) |
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If you have a problem its because you're spawn was dirty, not because of when you shake it or how often.
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 BEHOLD 
        
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Forevergreen
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Re: First grow. Spores to agar to grain to monotubs [Re: metaphoric]
#26835298 - 07/21/20 12:15 AM (3 years, 6 months ago) |
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They are clean and they are recovering already.
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Forevergreen
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Re: First grow. Spores to agar to grain to monotubs [Re: Forevergreen]
#26839205 - 07/22/20 08:21 PM (3 years, 6 months ago) |
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If anyone remembers I made some WBS jars. Only 3 showed growth and the one that had the most growth was an Ecuador and I threw it outside after smelling it. It smelled like pure mushrooms. It was put under a patch of bark so if 8 water it everyday hopefully some shrooms pop up? I only threw it outside is becauze I opened it. I should have g2g pr spawned it but oh well. Im learning what myc is. It had the yellow spots that turned out to be myc metabolism but you get so many people saying "its a contam" so I just threw it outdoors even though it wasnt contammed..
All the jars I shook have recovered and I am going to g2g tomorrow.
Edited by Forevergreen (07/22/20 10:41 PM)
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Phrontist
Determined



Registered: 07/17/13
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Re: First grow. Spores to agar to grain to monotubs [Re: Forevergreen]
#26839366 - 07/22/20 09:43 PM (3 years, 6 months ago) |
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If I can give you some advice I wish I was given right when I started: follow through on an entire grow process expecting to fail. Post pictures if you want, get advice, get feedback, store that knowledge for the future. For just one grow though, keep moving forward unless a Trusted Cultivator says you’re in danger of contaminating your entire home. You will learn infinitely more by DOING, even in failure, than by constantly second guessing and letting others control your grow via pictures. Who knows, you may even end up with some mushrooms to harvest at the end of it.
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F. 3
Flowing

Registered: 04/29/17
Posts: 192
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Re: First grow. Spores to agar to grain to monotubs [Re: Phrontist]
#26839841 - 07/23/20 06:14 AM (3 years, 6 months ago) |
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trust in dem mushrooms !
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