gizmo1 said:

Psilosopherr said:

Man, I swabbed like 7 species to agar and got no germination, just tons of contams.

They were all wild prints though. Dampened the swab with petri condensation before swabbing, prints are only like 2-3 years old, idk what the deal is. All the blank plates I poured in that batch are still clean, and I've had good success rates lately besides this.

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So you don't want to use old wild prints. Atleast I've never had luck with them. Ideally you should have cultivated them indoor while the prints were fresher then print them to store the prints. I'm not saying this is possible every time but it's best.
The reason being old spores take longer to hydrate and germ. Bacteria and mold doesn't. So if you have a old print that is "wild" it's gets harder and harder to get clean growth before bad stuff takes over. I'm not saying it's impossible at this point but unless they are super rare it's probably way more work than it's worth.

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jbaby007 said:

What dye do you use to get it so black?!

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Psilosopherr said:

gizmo1 said:

Psilosopherr said:

Man, I swabbed like 7 species to agar and got no germination, just tons of contams.

They were all wild prints though. Dampened the swab with petri condensation before swabbing, prints are only like 2-3 years old, idk what the deal is. All the blank plates I poured in that batch are still clean, and I've had good success rates lately besides this.

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So you don't want to use old wild prints. Atleast I've never had luck with them. Ideally you should have cultivated them indoor while the prints were fresher then print them to store the prints. I'm not saying this is possible every time but it's best.
The reason being old spores take longer to hydrate and germ. Bacteria and mold doesn't. So if you have a old print that is "wild" it's gets harder and harder to get clean growth before bad stuff takes over. I'm not saying it's impossible at this point but unless they are super rare it's probably way more work than it's worth.

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yeahhh, that was just what i had to work with. lots of valuable spores that'd take a lot of trading to replace with fresher. I'm particularly bummed the ovoid spores are showing their age, I really wanted to work with those guys. I was under the impression that they'd still be pretty good after a couple years, maybe i'll give it a second try.

I was certainly impressed when I swabbed an azurescen print that was only a week old, I got germination in like 3 or 5 days.

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Dephect said:

I can see how you might not like that.

I have ordered these plates a couple times. I like them but they too also have no ridges, it makes opening up for pouring really easy. I can run them through the PC and keep using them.

Do you have a link for those dishes? Can you run them through the PC?

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psycho_nauticus said:

This is my Burma culture I’m going to put to grain tomorrow or this weekend, I think it looks ready to go to grain. What do you guys think?

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