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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: psycho_nauticus]
    #26732714 -

psycho_nauticus said:
Holy crap, your germ plate?!?! Wow!!! And yeah, those are the same as the Holy Grail plates, I started to buy those as well and use them as no pours. I was able to also find 4 oz clear glass jelly jars, and cultures look so sick and clean in those as well.


Yeah, made a few transfers off of it and gonna let it pin out.

They grow like little putz, trying to find some with a more open pileus

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Re: AGAR ENVY! (Anything and All things agar!) [Re: oculodextro]
    #26732864 -

What are the pros and cons for high vs low nutes and soft vs firm agar? Is it true it's better to use soft agar when inoculating with swabs? Lastly, what do you look for in a plate before it's ready for a2g? Uniformity/no sectoring? Rhizo growth? Or as long as it is clean?

Edited by ouuwee (06/09/20 10:24 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ouuwee]
    #26733277 -

ouuwee said:
Lastly, what do you look for in a plate before it's ready for a2g? Uniformity/no sectoring? Rhizo growth? Or as long as it is clean?


Depends. If you wanna get something to grain asap, any clean plate (maybe except germ plates) will do. Lately people have been experiencing that tomentose myc can be just as good of a fruiter as rhizo myc.
If you want to have an isolate or something close to it before you put it to grain, wait until your growth shows no sectoring.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: PMBastian]
    #26733377 -

PMBastian said:
ouuwee said:
Lastly, what do you look for in a plate before it's ready for a2g? Uniformity/no sectoring? Rhizo growth? Or as long as it is clean?


Depends. If you wanna get something to grain asap, any clean plate (maybe except germ plates) will do. Lately people have been experiencing that tomentose myc can be just as good of a fruiter as rhizo myc.
If you want to have an isolate or something close to it before you put it to grain, wait until your growth shows no sectoring.


I've even dropped germ plates that were clean... lol. Also, seems a lot of peeps that are new to agar all want an isolate or a monoculture... those things are great if you pulled clones and did xfers and all that yourself... you're not gonna get an isolate overnight...

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Re: AGAR ENVY! (Anything and All things agar!) [Re: roarkell]
    #26735110 -

I'm a little worried. My T2's are being super slow. 6 days and they've hardly jumped off the transfer wedge and they look quite whispy and light. Should I be concerned?

Lousy pic included



--------------------
Make my Funk the P Funk, I wants to get Funked up

LAGM2024

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Gastronomicus]
    #26735124 -

Could just be the lighting, but makes me want to use the m-word


--------------------
It's so easy to laugh
it's so easy to hate
It takes guts to be
gentle and kind
-the smiths

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight]
    #26735160 -

Me too  :facepalm3:

I tossed all the T1's too like an idiot. I still have the germ plate though and it's looking fine. I took a provisional transfer from it earlier today so I might not be too far behind the eightball.


--------------------
Make my Funk the P Funk, I wants to get Funked up

LAGM2024

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Gastronomicus]
    #26735844 -

What's your agar recipe?


--------------------
How I do glass dishes

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Re: AGAR ENVY! (Anything and All things agar!) [Re: poisoned]
    #26735908 -

1.5% BRF 2% Agar and a drop of green food coloring.


--------------------
Make my Funk the P Funk, I wants to get Funked up

LAGM2024

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Re: AGAR ENVY! (Anything and All things agar!) -- cobweb? [Re: Tormato]
    #26736130 -

little intrusion.

I've made a transfer from this petri:


to this one:


As you can see there are two spot of something growing,
this is the piece of agar that I've transferred:

I see the mycelium too fluffy, maybe is cobweb since is there another spot?


I'm near the 'agar to jars moment', but idk what to do with these dishes


--------------------
Only a lot of stories...

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Re: AGAR ENVY! (Anything and All things agar!) -- cobweb? [Re: panoramics]
    #26736136 -

I'll wait for someone more experienced, but I think
yer gonna get schooled for using imgur...I could be wrong...

When you upload pics to your Pics area, personal data is stripped from the pic.
And you can create Folders to keep yer pics organized.

Edited by Inthepit (06/11/20 07:31 AM)

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Re: AGAR ENVY! (Anything and All things agar!) -- cobweb? [Re: panoramics]
    #26736137 -

That second pic could be mold.

Anytime I see a little spot with a bright white dot in the middle it’s been mold. Especially since it’s near the edge. Keep an eye on it and do something before it sporulates.

^^^also that. People won’t click shit off site.


--------------------
LAGM2020

Edited by A.k.a (06/11/20 07:20 AM)

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Re: AGAR ENVY! (Anything and All things agar!) -- cobweb? [Re: Inthepit]
    #26736241 -

I didn't know that our personal info was stripped. Good looks buddy :thumbup:


--------------------
Anything and everything  I say, write, and/or imply is purely false and is only done so with the intent to enhance my acting career.

Molon Labe

As above, so below

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Gastronomicus]
    #26736257 -

Ive read that some people try to have a single spore culture which I suppose they want to link up with another single spore.

Can you see a difference in the growth or mycelium characteristics between monocaryon and dicaryon or is it plausable to assume that if you used a syringe or spore swab that the mycelium growing is dicayotic?

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Re: AGAR ENVY! (Anything and All things agar!) -- cobweb? [Re: Inthepit]
    #26736638 -

Inthepit said:
I'll wait for someone more experienced, but I think
yer gonna get schooled for using imgur...I could be wrong...

When you upload pics to your Pics area, personal data is stripped from the pic.


Sure, but you can remove your data before the upload on Imgur...:eek:


Quote:
And you can create Folders to keep yer pics organized.



Good to know


--------------------
Only a lot of stories...

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Re: AGAR ENVY! (Anything and All things agar!) [Re: p1x1e]
    #26736649 -

A.k.a said:
That second pic could be mold.

Anytime I see a little spot with a bright white dot in the middle it’s been mold. Especially since it’s near the edge. Keep an eye on it and do something before it sporulates.

^^^also that. People won’t click shit off site.


ok thanks, so probably i'll throw the dish


p1x1e said:
Ive read that some people try to have a single spore culture which I suppose they want to link up with another single spore.

Can you see a difference in the growth or mycelium characteristics between monocaryon and dicaryon or is it plausable to assume that if you used a syringe or spore swab that the mycelium growing is dicayotic?



think I have to wait a little bit. don't know what dicayotic means


--------------------
Only a lot of stories...

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Re: AGAR ENVY! (Anything and All things agar!) [Re: panoramics]
    #26736911 -

Dikaryotic..
A famous section about mushroom sex in older literature.
Mind you, I am reading here that tomentose can also produce fruit and not just rhizomorphic?
So maybe im worrying about things that is really insignificant.
It just gets frustrating as fuck when you're not entirely sure what to expect,follow a description best you can, tick the boxes, get pretty damn close
BUT "something" is not right

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Re: AGAR ENVY! (Anything and All things agar!) [Re: panoramics]
    #26736954 -

It is not all about getting things done, failing but understanding it's even better; something it's not right.

so yes, I have a lot to study.


--------------------
Only a lot of stories...

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Re: AGAR ENVY! (Anything and All things agar!) [Re: panoramics]
    #26737073 -

I agree that it is very rewarding to break through and discover something that you been at for a long time.
On the other end, the wheel has been invented ages ago..there's really no use in re-inventing it

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Re: AGAR ENVY! (Anything and All things agar!) [Re: p1x1e]
    #26737984 -

p1x1e said:
Dikaryotic..
A famous section about mushroom sex in older literature.
Mind you, I am reading here that tomentose can also produce fruit and not just rhizomorphic?
So maybe im worrying about things that is really insignificant.
It just gets frustrating as fuck when you're not entirely sure what to expect,follow a description best you can, tick the boxes, get pretty damn close
BUT "something" is not right


Tormentose vs rhizomorphic often relates to the amount of nutrient in your agar. . . High nute often makes tormentose as the myc isn't in a hurry as it has plenty of food. . .
Rhizomorphic happens when there isn't much nutrient and the myc is on the hunt for food. . .


--------------------
The Official Kiwi Cultivators Thread

Official New Zealand woodlovers grow thread

–––––    ╭∩╮(-_-)╭∩╮    –––––
The Official Kiwi DMT and MAOI Thread

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