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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Shop: Sporeworks.EU Spores for European Microscopy   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures

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glove box question for newbie
    #2663704 -

while working inside a sterilized glove-box w/ 12 jars,,, dose one have to keep flaming the needle for every jar or is once enough???
w/ the edition of wiping the needle w/ alchol pad in between jars.

thanx, MD


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BETCHA CAN'T EAT JUST ONE,,,GET YOUR OWN BAG!!!

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Re: glove box question for newbie [Re: MeNtALDeMoNZ]
    #2663711 -

No it isnt neecessary but why scimp alchol between holes and flame between jars safer is better.


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Re: glove box question for newbie [Re: FallenShroom]
    #2663719 -

Yes I agree,a little alcahol prep pad in your glovebox to wipe the needle is a good idea.If for some reason one of the jars did contain contams,whether your in a glovebox or not you could still spread them from jar to jar.


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Butternut squash are softer than acorn or other types of squash, which makes them easier to cut and prepare.

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Re: glove box question for newbie [Re: FallenShroom]
    #2663722 -

ok thanx for the insight


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BETCHA CAN'T EAT JUST ONE,,,GET YOUR OWN BAG!!!

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Re: glove box question for newbie [Re: MeNtALDeMoNZ]
    #2664086 -

i do mass inculation for jars at 1 time and for me to flame the needle each time is just to much the needle eventully starts getting wobbly lol i just use a thick towel and lots of 91% achol and thats not even in a glove box i have a flow hood tho

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Re: glove box question for newbie [Re: tripin420]
    #2672663 -

i know tripin420 to flame the needle for each and every jar seems like over-kill while in a sterilized glove-box,,, and yes the needle melts the plastic of the syringe flameing for each jar!?!

dose anyone else have insight to this ???


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BETCHA CAN'T EAT JUST ONE,,,GET YOUR OWN BAG!!!

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Re: glove box question for newbie [Re: MeNtALDeMoNZ]
    #2672687 -

we already told you,there is no need to do that if your in a glovebox,but alcahol is still a wise precaution.


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Butternut squash are softer than acorn or other types of squash, which makes them easier to cut and prepare.

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Re: glove box question for newbie [Re: ButterNut]
    #2672702 -

thanx butter! will do !


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BETCHA CAN'T EAT JUST ONE,,,GET YOUR OWN BAG!!!

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Re: glove box question for newbie [Re: MeNtALDeMoNZ]
    #2672734 -

  :goodluck:


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Butternut squash are softer than acorn or other types of squash, which makes them easier to cut and prepare.

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Re: glove box question for newbie [Re: ButterNut]
    #4227107 -

This may be overkill, but it makes for peace of mind.

What I did was create a glove box (did a shit load of other precuations too) and inside I wipped the lid of each jar with an alcohol pad so if I touched the needle to the lid it wouldn't carry germs. Then I flamed the needle once before every jar and then wipped with the alcohol pad to cool it down. Each jar had four holes, and every time I took the needle out I did a quick wip.

So it was really one flame per jar, four alcohol wips per jar, and one nice big wip on the surface of the jar.

If you want to see how crazy I really am, I wrote a long post on what I did:
http://www.shroomery.org/forums/showflat...rue#Post4211385


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Shop: Sporeworks.EU Spores for European Microscopy   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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