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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Experimenting with hydrogen peroxide for bacterial issues * 1
    #26622167 -

Hey guys. I've been working on my first grow for a long time now, around two months or so, and my problems were mainly bacterial issues (fruity smell and then blobbing) and stalling, with a very very slow growth rate. At first I thought it was my spore syringe, then my G2G technique and finally my pasteurization technique. So I tried making few shoeboxes more but seemed to fail again, even with improving sterile technique. Made lots of shoeboxes and every single one of them contammed with bacteria.
Last week I gave a chance to something Gordotek does, using 3% hydrogen peroxide on the casing. So I've tried doing the same thing I've been doing (SFF Shoebox Tek) but this time experimenting with two containers at the same time and see the difference, spraying one of them with mineral water (as I did all this time) and the other one with the hydrogen peroxide 3%.
This is the picture. The above one is the one with hydrogen peroxide, and the one below is with mineral water. The pic explains itself.

Yesterday I opened both, and the H2O2 one does not smell agressive at all, nor has the typical mushroomy smell yet, but I'm sure if I give it FAE it would appear. And the one below it had a pretty pretty strong bacterial smell, exactly the same I've been dealing all this time.
I asked a buddy what he could expect it to be and we started talking about our coirs. His is the one that you all use, the hard dry block that you just add water and thats it. On the other hand, I've been using all this time the hydrated one. He tried doing some research of my coir and found that mine has tricoderma in it even when it should NOT have it in it, because the package doesn't say that; the bag clearly says 100% coco coir. This same brand sells apart coir with tricoderma added in another different package, so I assumed I could trust the label (100% coir) and just pasteurize it.
So I'm pretty happy to find the solution I've been searching for quite a lot time. This time I think I could fix this bacterial issue using hydrogen peroxide. Any trusted cultivator could confirm if H2O2 could treat tricoderma?
Lastly, I have around 20qt left of this coir, so I will try sterilizing it (instead of pasteurizing as I've done all this time) and misting with hydrogen peroxide. The next time I will just buy the dry block of coir and prevent all this headache.


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Edited by gabbk (04/23/20 02:33 PM)

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Re: Experimenting with hydrogen peroxide for bacterial issues [Re: gabbk] * 1
    #26622182 -

Peroxide doesn’t kill trich that’s already growing, I don’t know about how it might affect ungerminated spores if that’s what is in that coir you get. I would definitely get the eco earth bricks asap, it’s good stuff.

The above pic definitely looks better but you shouldn’t really have to spray a colonizing tub if your moisture content is correct. The one on the bottom looks way wet, maybe just the pic.

Do you ever germinate your spores on agar or have you been going syringe to grain every time?


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Sour grapes, sweet revenge
Heaven starts right where hell ends

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Re: Experimenting with hydrogen peroxide for bacterial issues [Re: Roger Clemency]
    #26622188 -

H2O2 has no place in this hobby, at least not anywhere close to your myc. Instead of treating your coir, I suggest you to start with agar next time.


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How I do glass dishes

Edited by poisoned (04/23/20 02:05 PM)

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Re: Experimenting with hydrogen peroxide for bacterial issues [Re: Roger Clemency]
    #26622205 -

I do work with agar, and all these containers were filled with G2G, from the same healthy master jar, and specifically this container sprayed with H2O2 had this particular healthier result than all the other ones with mineral water or even bleach water (yes, I even tried hippie3 bleach technique), that they were all very slow: these spawns took around 20 days to colonize around 70%, and this one with H2O2, within just 7 days its going a lot better than the other ones did.
I know in theory H2O2 has 'no use' here. But I just wanted to give it a shot and apparently in this specifical case worked. I will try leaving the other container outside to get air and dry it out a little bit and then apply H2O2 and see how it evolves.


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Edited by gabbk (04/23/20 02:17 PM)

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Re: Experimenting with hydrogen peroxide for bacterial issues [Re: gabbk]
    #26622207 -

I already posted some other threads asking for opinions on my jars, how they looked. And they were fine. The issue I think was the coir, that being hydrated carried within it bacteria.


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Re: Experimenting with hydrogen peroxide for bacterial issues [Re: gabbk]
    #26622228 -

I've been having a similar problem with contam'. I'm considering putting my coir into a strainer and pressure cooking it.

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Re: Experimenting with hydrogen peroxide for bacterial issues [Re: GoodDoctor06]
    #26622252 -

Worth to say again that if you look to my other posts, this is JUST BACTERIAL contamination, not fungal or molds. There are fruits growing normally. But the thing is the odor and yellow metabolites floating on the substrate that prevents maximum yielding possible.


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Re: Experimenting with hydrogen peroxide for bacterial issues [Re: gabbk]
    #26622256 -

Like bod says

hydrogen peroxide is useless

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Re: Experimenting with hydrogen peroxide for bacterial issues [Re: Whyterye] * 1
    #26622272 -

Whyterye said:
Like bod says

hydrogen peroxide is useless


Why do we keep having to say this?
:knowwhatnevermind:


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Pastywhyte's easy no pour agar dishes

Stallion Mang’s Ween covers

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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