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Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour, Rye Grain

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Offlinefelix
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Re: 2-liter "jars" of substrate?
    #26286 - 07/31/00 01:48 AM (16 years, 4 months ago)

big ass waste of time.


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InvisibleKid
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Re: 2-liter "jars" of substrate?
    #26287 - 07/31/00 12:48 PM (16 years, 4 months ago)

There is a tek on this site about sterilizing everything in the microwave. Try finding a microwave safe container. It's the shape of the pop bottle that makes me think this is a very good idea. You sterilize the whole bottle, take off the cap, then inject through the top. This way the spores will trickle down and spread out through the substrate, maybe speeding colonization.

A pop bottle won't work in the nuker though, it'll melt.

I've also wondered about the viability of a huge jar of substrate. I know a few teks vary from the standard 1/2 pint jar to 1/4 pint or as larg as quarts (just over 1 litre).

The one thing that might be crucial (don't go by my word though) is to add more moisture than most recipies ask for, because with a jar that size, you want to make sure you utilize the whole substrate and maximize the amount of flushes.

tx



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OfflineKrupa
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Re: 2-liter "jars" of substrate?
    #26289 - 07/31/00 03:58 AM (16 years, 4 months ago)

If you can get it sterilized, I don't see why it would be any worse than a jar. The big problem is going to be getting it to colonize with mycelium before contams. LOTS of spore water. If someone can think of a way to sterilize it, I'll try it.


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OfflinePyrotechnist
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Re: 2-liter "jars" of substrate?
    #26290 - 07/31/00 04:19 AM (16 years, 4 months ago)

Hmm.. this makes me wonder whether substrate can have a 'critical mass,' so to speak. Because if you didn't get spores into the center of the substrate and you just got them on the outside.. well, remember this cake would have a volume of two liters. The mycelium at the edges might have totally consumed the substrate at the edges by the time the center got colonized.

I think it'd be a big waste of time and spores. But go ahead and try it anyways if you want, it's all in the name of science.



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OfflineAnnoA
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Re: 2-liter "jars" of substrate?
    #26291 - 07/31/00 07:37 AM (16 years, 4 months ago)

Big substate amounts only make sense if you can spread the mycelium or spores through the substrate. In case of rye you simply shake the jar after the innoculation and then again after a few days to redistribute the growing spots of mycelium. But there is also a limit because of the heat and the gases the mycelium generates while growing. The biggest containers for spawn production used AFAIK have app. 3 galons.
A 2 galon PF-style cake though would probably newer colonise compleately.


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OfflineFun-Guy
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Re: 2-liter "jars" of substrate?
    #26292 - 07/31/00 10:19 AM (16 years, 4 months ago)

quote:
Originally posted by Anno:
Big substate amounts only make sense if you can spread the mycelium or spores through the substrate. In case of rye you simply shake the jar after the innoculation and then again after a few days to redistribute the growing spots of mycelium. But there is also a limit because of the heat and the gases the mycelium generates while growing. The biggest containers for spawn production used AFAIK have app. 3 galons.
A 2 galon PF-style cake though would probably newer colonise compleately.

where in the hell would you get this 3-gallon deal. and is it glass or plastic?



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OfflineAnnoA
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Re: 2-liter "jars" of substrate?
    #26293 - 07/31/00 11:18 AM (16 years, 4 months ago)

The big containers are actualy bags made from heat resistant plastic.




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Anonymous

Re: 2-liter "jars" of substrate?
    #26294 - 07/31/00 11:22 AM (16 years, 4 months ago)

Two liter will work fine. Do not use spores though use mycelium covered agar. My friend has 1 gallon jars that she uses and uses agar to inoc. Use whole grain rye berries so you can shake it up. Wont make a cake but will colonize in bout 8 to 10 days.


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OfflineKrupa
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Re: 2-liter "jars" of substrate?
    #26295 - 08/02/00 12:53 PM (16 years, 4 months ago)

Grain would be super. Sterilization is the biggest problem so far. Like I said before, I'll waste a syringe by using it on BRF substrate in a 2L bottle if someone figures out how to sterilize it. PET has kind of a low melting point, so it's a real toughie...


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OfflineAnnoA
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Re: 2-liter "jars" of substrate?
    #26296 - 08/02/00 01:47 AM (16 years, 4 months ago)

Why do you have to use a PET bottle?
Better try finding a big jar.


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OfflineKrupa
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Re: 2-liter "jars" of substrate?
    #26297 - 08/02/00 04:36 PM (16 years, 4 months ago)

I was only refering to 2L pop bottles. Large jars work very well, I agree.
It'd just be kind of fun to have a 2L full of colonized substrate.
Games:
If it's large amounts of substrate you're looking for, then I would go for very large jars of grain. If it's just for fun, I wish you the best!


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Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour, Rye Grain

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