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How to cultivate 5000 doses worth of psilocybin in a small room every week Tek * 1
    #26191459 -

So I found an old thread that refuses to be revived that details a method of growing huge quanties of mycelium on soft agar and extracting the actives. It had been lost in translation and beatifully rediscovered.

Yet no interest. I have no idea why. Maybe the title is too boring and the text too thick and off putting.

Here's the link. I think it's worth a look, but I'm an absolute begginer with first pf cakes on the way. So maybe it's useless. Keen to see how plausible you all think this is?
Edit: oops, 3am forgot to add the link https://www.shroomery.org/forums/showflat.php/Number/7680813#7680813

Edit for more concise and better writing collected together:

Hi all :smile:
I have never been an active member here or of the DMT nexus, occasional long time lurker. These threads referenced can be found at the bottom of the post.

I not long ago I found an old post of an older tek that had been long forgotten and thought to be bunk due to a reportedly terrible translation.

https://www.shroomery.org/forums/showflat.php/Number/7680529

The Brack and Kobel method of psilocybin production.

The claim is that mycelium in some mushrooms (maybe not pan cyans, as one member showed a law enforcement study of pan cyans that tested no psilocybin prior to pinning, though consuming pre-fruited mycelium of Cubes anecdotally produces effect) is active, and it's speed of growth makes it a viable option for rapid actives production. The mistranslation is generally interpreted as myc grown in LC.
However, LC myc catabolises it's actives very quickly at exactly the one week mark and requires close and difficult (complex, apparatus for testing) monitering to be harvested in the small optimal window. The other common mis-interpretation is to grow on hard agar. This results in myc that is unable to penetrate the surface, thereby providing too thin a layer of myc, low yields.

The solution posed is that agar mixed to 1/10th the dilution of hard agar allows for mycelial penetration. and one ought to harvest once the myc has carpeted the surface.

Reportedly effectively done by Brack and Kobel whilst working for sandoz in the 50s, also effectively used to grow sclerotia and fruit from this substrate. The sclerotia is extremely clean and easily harvested, and the agar is rightly priced if using it at 1/10th regular dilution.

The corrected translation of the tek recieved very little interest, admittedly the title and body of text is offputtingly dense in the old post.

I am curious to see if this is a viable tek for producing actives at 6x the speed. (So much more in the fruit, I realise, still I see potential benefit here, grow bulk then and extract to another medium if eating heaps of myc is unappealing, the tek also mentions an extraction tek (I know fast degradation, make some agar jellies with it or mix it with some other protective medium, add flavours too :smile: )

I tried to revive the old thread to little avail. Then I tried making a post in the cultivation thread, mixed responses. A lot of ill will toward the concept. A good point was made by footpath; "Just about everyone knocks what they don't try and don't even take time to read anything legitimate about. They mainly just proliferate speculative rumors from the populous. Ultimately, I am 99% certain that 99% of the people here Do Not have the means to produce anything beyond anecdotal evidence. And that, most of the time, they don't even bother to create that."

Now, please, forgive my arrogance for making three posts on the same topic and necroing an 11 year old thread about an abandoned tek from the 50s. I am curious, and see potential benefit to the community at large, perhaps the advanced crowd will be more likely to share in my curiosity.

I will also post this to the DMT nexus.

I am a noob. I know nothing. I am in process of growing my first PF jars. I have no experience with agar. I am a student on government assistance who has just been kicked out of home and am shortly to live in a tent in the backyard of my law abiding Aunt. My pf jars have had to be moved to another location 3 hours from my Aunts. There will be renovations there in a months time for a month and a half. My jars will have to be spawned to completely autonomous bulk monotubs on my first grow.

I am an avid reader and have a lot of curiosity, that is just about all that is going for me as a cultivator.

If anyone feels any curiosity toward such a beautiful tek, a week, a little agar and some curiosity is the minimum requirements for some results. I believe that others may have favourable conditions, supplies and skills for such experimentation earlier than I will be able to.

Have a go if you will and tell us all how you go :smile:


Here are my other threads in the cultivation sections of the shroomery, the DMT nexus and again, the old post I refer to;

Look at this, promising anecdotal reports regarding growing strong, active azurescens mycelial mats on agar. Easier than sclerotia even and better substrate for sclerotia too.
https://www.shroomery.org/forums/showflat.php/Cat/0/Number/7789758

Spent cake tea, effective -
https://www.shroomery.org/forums/showflat.php/Number/14257992#14257992

Eating cakes and mushroom stem bases on surface of cakes -
https://www.shroomery.org/forums/showflat.php/Number/14992441#14992441

Mycelium extraction teks -
https://www.shroomery.org/forums/showflat.php/Number/539138#539138

https://www.shroomery.org/forums/showflat.php/Number/4387553#4387553

General cultivation shroomery thread -
https://www.shroomery.org/forums/showflat.php/Number/26191459/fpart/1/vc/1

Advanced cultivation thread -
https://www.shroomery.org/forums/showflat.php/Number/26192695

Law enforcement study on what stage of Pan cyan myc contains actives, with pan cyans they found that it isn't until pinning, note, I've heard reports of other species that contain notable actives prior to pinning and this is the only source I found to say this about pan cyan, we trust cops right haha-
http://www.fanaticus.com/forensic.htm

The old post on the supposedly correctly translated Brack and Kobel method -
https://www.shroomery.org/forums/showflat.php/Number/7680529


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All's good :smile:

Edited by ThePurpleWiggle (09/16/19 09:07 PM)

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Re: How to cultivate 5000 doses worth of psilocybin in a small room every week Tek [Re: ThePurpleWiggle] * 2
    #26191463 -

Its much easier and effective to just grow the mushrooms :shrug:


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How I make and preserve tea


Consuming consumes a man That was never a purpose of life To only crave for material joys Is believing the lie - Mellow Mood

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Re: How to cultivate 5000 doses worth of psilocybin in a small room every week Tek [Re: SFS96] * 1
    #26191467 -

Yet takes 6 times as long.


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All's good :smile:

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Re: How to cultivate 5000 doses worth of psilocybin in a small room every week Tek [Re: ThePurpleWiggle] * 1
    #26191470 -

Would go alright with azure or cyan myc.


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All's good :smile:

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Re: How to cultivate 5000 doses worth of psilocybin in a small room every week Tek [Re: ThePurpleWiggle] * 1
    #26191474 -

As far as I'm aware nothing terribly useful can be extracted from the mycelium.  Many places do make extracts from mycelium, Fungi perfecti being one of them. Those products are pretty much bunk :shrug:

Actives are not found in mycelium, which is what makes it legal to grow in Canada.

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Re: How to cultivate 5000 doses worth of psilocybin in a small room every week Tek [Re: Stipe-n Cap] * 1
    #26191485 -

There are plenty of actives in mycelium.

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Re: How to cultivate 5000 doses worth of psilocybin in a small room every week Tek [Re: Mycolorado] * 1
    #26191497 -

So, differing opinions already. Maybe it'd be worth while to grow some azure myc on agar small scale and see if pre fruited myc actually has a psychoactive effect? It wouldn't have to be near as strong as fruit by any means for the 6x speed boost to be worthwhile.

Edited by ThePurpleWiggle (09/16/19 11:32 AM)

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Re: How to cultivate 5000 doses worth of psilocybin in a small room every week Tek [Re: Mycolorado] * 1
    #26191500 -

Quote:
There are plenty of actives in mycelium.



Agreed.  You can trip from breaking up a colonized grain jar and soaking it for a little while in cranberry juice. 

The psilocybin producers guide is something no one has been able to get to work, AFAIK.

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Re: How to cultivate 5000 doses worth of psilocybin in a small room every week Tek [Re: Mycolorado] * 2
    #26191504 -

ThePurpleWiggle said:
Yet takes 6 times as long.



6 times longer for probably 100 times more actives. Seems like a fair trade :jonespalm:


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Re: How to cultivate 5000 doses worth of psilocybin in a small room every week Tek [Re: JohnRainy] * 1
    #26191506 -

True, but that thread ckeared up a serious translation issue regarding the agars softness. It only had 12 replies, leads me to think that the correct teanslation is very little known.


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All's good :smile:

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Re: How to cultivate 5000 doses worth of psilocybin in a small room every week Tek [Re: ThePurpleWiggle] * 2
    #26191512 -

So much speculation, an experiment would literally take one week. I may try in the not so so distant future.


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All's good :smile:

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Re: How to cultivate 5000 doses worth of psilocybin in a small room every week Tek [Re: ThePurpleWiggle] * 1
    #26191521 -

thread should b called how to go to jail in a week


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PANAEOLUS FRUITING MADE SIMPLE


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Re: How to cultivate 5000 doses worth of psilocybin in a small room every week Tek [Re: ThePurpleWiggle] * 1
    #26191528 -

There's no speculation. That thread is 11 years old. No one does that because there are ways 100x better. It kinda sounds to me like you can't get anything to fruit or you'd not be trying to extract actives from mycelium. Just grow some shrooms


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Re: How to cultivate 5000 doses worth of psilocybin in a small room every week Tek [Re: Mycolorado] * 1
    #26191530 -

Mycolorado said:
There are plenty of actives in mycelium.



Plenty? I would assume that some would be present, not enough to be worth mentioning. Almost like saying that hemp contains "some" thc. I can't seem to find a source however.

Care to elaborate?

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Re: How to cultivate 5000 doses worth of psilocybin in a small room every week Tek [Re: ThePurpleWiggle] * 2
    #26191537 -

Or someone who doesn't live in a tent in a rellos backyard, who has agar and experience working with it, has fruited at least a single shroom, could attempt a tek that was tried and reportedly successfully used to grow fruits and sclerotia on top of active myc (very easily cleaned and harvested stones btw, also sandoz was interested and effectively did it if I recall). Also my grow space is 2-3 hours away and theres going to be renos next month for a month and a half there and I will have to use totally autonomous monotubs for my pf cakes.

Please, can an experienced grower help to sate my curiosity and potentially pioneer psilocybins increasing presence in the world?


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All's good :smile:

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Re: How to cultivate 5000 doses worth of psilocybin in a small room every week Tek [Re: ThePurpleWiggle] * 2
    #26191543 -

Quote:
JOURNAL OF FORENSIC SCIENCES
American Academy of Forensic Sciences (1948)
Volume 45 - Number 3 - May 2000 - JFSCAS 45 (3)513-754 (2000)

Susan T Gross, B.A.
Forensic Scientist, Minnesota Forensic Science Laboratory, 1246 University Ave. St. Paul, Minnesota.

This project was supported by the Federal Bureau of Investigation (FBI). Minneapolis Field Office.

Received 26 Feb. 1999: and in revised form 12 May 1999: accepted 23 Aug. 1999.

REFERENCE: Gross ST. Detecting psychoactive drugs in the developmental stages of mushrooms. J Forensic Sci 2000;45(3):527-537.

ABSTRACT: The following questions regarding the detection of psychoactive drugs in mushrooms are
addressed: At what stage of the mushroom development can the psychoactive drugs psilocyn and psilocybin be
identified, and what effect does light have on the growth of these mushrooms. To answer these questions,
Psilocybe cyanescens Wakefield mushrooms were grown from their spores in a controlled setting. At various
times of their development, samples were taken and analyzed for psilocyn and psilocybin. Knowing what stage
of development the psychoactive drugs can be identified may be useful to law enforcement personnel and
forensic chemists. Methanolic extracts of various samples were analyzed by TLC and by GC/MS. It was
determined that the mycelium knot stage of the mushroom was the earliest stage at which the psychoactive
drugs could be detected. It was observed that light affected the time of development and the appearance of
these mushrooms.



If you're going all the way up to knotting, might as well fruit. This would be akin to saying that mycelium doesn't really contain actives.

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Re: How to cultivate 5000 doses worth of psilocybin in a small room every week Tek [Re: Stipe-n Cap] * 1
    #26191551 -

I and plenty of people have tripped balls off spawn. :shrug:  The study you’re showing was done on cyanescens.  Im referring to cubensis.

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Re: How to cultivate 5000 doses worth of psilocybin in a small room every week Tek [Re: ThePurpleWiggle] * 2
    #26191554 -

ThePurpleWiggle said:
Please, can an experienced grower help to sate my curiosity and potentially pioneer psilocybins increasing presence in the world?



Again? Just grow some shrooms for fucks sake.  :wtfno:


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Re: How to cultivate 5000 doses worth of psilocybin in a small room every week Tek [Re: ThePurpleWiggle] * 1
    #26191562 -

I'm on my first pf tek jars still colonizing (so slow, just made the hike to my cakes to drop of a heater), 11 years old with no reported attempts. The beauty here is the speed and ease of the tek, snd uses a tenth of agar as normal hard stuff, so the price is right. A week is all it takes.

Interesting source, yet still, it conteadicts the original tek, perhaps mexicana, azures or cubes would be different. I read a post of a guy done for have colonized cakes OG charged for intent to supply psilocybin, tests changed the charge to psilocin as no psylocybe was found, yet psilocin was.

I am growing attempting to grow shrooms for my first time 'again'.z


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All's good :smile:

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Re: How to cultivate 5000 doses worth of psilocybin in a small room every week Tek [Re: Mycolorado] * 1
    #26191565 -

Roger that.

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