Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery


This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Autoflowering Cannabis Seeds   North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   Sporeworks.EU Spores for European Microscopy

Jump to first unread post Pages: | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Re: I seriously don't get it. [Re: 10kVisions]
    #26012467 -

10kVisions said:
bodhisatta said:
10kVisions said:
bodhisatta said:
Try doing agar with something other than mason jars for starters.


They’re not pint jars Bod.  I’m using pasty whites agar Tek. But instead of the plastic small containers I use the ball glass version. Same size etc just glass instead of plastic.


yea I know.

19 for 2 should be just fine at 10k



Thanks.  Let me ask you this.  How would I reheat an agar bottle with no microwave?  Most people make a bottle, sterilize it and then pour each Petri dish then to re heat they use a microwave. I don’t have a microwave.  I do pasty whites tek because I can make the jars and then sterilize them


Make agar plates the same day you sterilize the agar :shrug: I've melted agar with the microwave at home probably three times ever. I almost always just make plates right after sterilizing the agar so it never has to get re melted.


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

Extras: Filter Print Post Top
Re: I seriously don't get it. [Re: bodhisatta]
    #26012562 -

Quote:
Make agar plates the same day you sterilize the agar :shrug: I've melted agar with the microwave at home probably three times ever. I almost always just make plates right after sterilizing the agar so it never has to get re melted.


ya that's basically what im doing, i make the agar. put into plate, PC the plates.


OK so im going to try something suggested here

i have a bathroom thats barely used in the house so today i'm going to make 4 half full WBS jars.  2 i'll use Poly Fill on and 2 i'll use Micropore tape on.

afer i PC them i'll put 2 of them in the room i built and 2 of them in the Bathroom both temps will be between 68 and 72º

i'm going to let them sit un opened un touched and see if anything grows.
does anyone have an idea of how long i'll need to wait before i start to see something?  not that im in a hurry i just like to let things sit un disturbed and check when its needed.

im deteremined to figure this out cause something is definitely going on.

while that is happening im knocking about 20 new plates today in the room i built under a SAB with alcohol cleaned surfaces etc. i'll post a photo of the setup once i get started.


--------------------

Extras: Filter Print Post Top
Re: I seriously don't get it. [Re: 10kVisions]
    #26012566 -

Im suggesting using normal petri dishes to make your time with agar as easy as possible. Do agar in Tupperware once you build skill and dexterity


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

Extras: Filter Print Post Top
Re: I seriously don't get it. [Re: bodhisatta]
    #26012573 -

bodhisatta said:
Im suggesting using normal petri dishes to make your time with agar as easy as possible. Do agar in Tupperware once you build skill and dexterity


ya i got that, the jars aren't plastic Tupperware.  i used his tek but changed that to the glass versions of those same size Tupperware simply cause i know it will be cleaner.  i've had great success with my last 2 agar grows but i think my problem was my wedge cuts where big and i was transferring contams without knowing it.  i'll be ordering Petri dishes this week as well only cause they also just seem easier to work with in general.

i am super curious if there is more than just this as a problem too.  so i'd like to try some stuff suggested on here and see if i can find anything outside of the agar thats causing more issues.

thanks for the advice.


--------------------

Extras: Filter Print Post Top
Re: I seriously don't get it. [Re: 10kVisions]
    #26012580 -

Look at the josex poke method. But im like mad season i just do small transfers


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

Extras: Filter Print Post Top
Re: I seriously don't get it. [Re: bodhisatta]
    #26012664 -

bodhisatta said:
Look at the josex poke method. But im like mad season i just do small transfers


that is an interesting take but i feel i like your tek best honestly. its been the easiest to grasp for me.


--------------------

Extras: Filter Print Post Top
Re: I seriously don't get it. [Re: 10kVisions]
    #26012914 -

OK so here is pics from today.

in the bucket I put 3 jars of WBS.  filled with hot water and straied out all the floaters, then refilled with hot water and will let sit for 24 hours. The purpose here is to test the PC Time and air quality issue.  I will take this seed, make 4 1/2 full jars, 2 with poly fill and 2 with Micropore tape.  Then I will PC them at 19psi for 2 Hours.  when done, I will place one of each jar type in 2 different ares.  1 will be in the room I built specifically for doing this, the other will be in a bathroom that doesn't get used much and will be easy to maintain a good temp in.  None of the jars will be opened, or knocked in anyway. this is PLAIN WBS to see if anything grows that could be considered contams that way I can narrow down my issues.






AGAR:

my tools:


My SAB setup.  I use a plastic bag (new) that I spray with alcohol, then I spray the inside of the SAB with alcohol and place on top of the bag and let sit for 10 min or so.


My Agar out of the PC.  this sat in this room in a box (sealed) over night to cool down to room temp.  room temp is 71º


procedure: (gloves warn at ALL times)

heat syringe, cool down with a couple drops into SAB.  then 2 drops into agar, replacing lid when done.
pick up looper, heat red hot, open agar lid, cool looper in agar then spread drops into z formation and close lid this time screwing lid down and placing jar aside (still in SAB)

done and on the shelf



--------------------

Extras: Filter Print Post Top
Re: I seriously don't get it. [Re: 10kVisions]
    #26012951 -

Your transfers are waaay to big. How do you hold the plates? They should start upside down but you have to twist off the top making those dumb *redacted other sites name* jars bad for agar. Read through the threads at that fail ass site, lots of "awesome ideas" and "facts" thrown around with no evidence or grow pictures and 90% of threads are open ended due to failure, the OP never reports back. The guy that runs that place uses a tub with a 5 dollar fan and 12 dollar filter as a flow hood, he believes this works.

TLDR-pasty plates work because they are plastic. Look up *Tiger Drop* watch the videos and grow some healthy samples.

**No one ever asked, how long are you PC'ing the agar? If LME agar I would suggest 45 minutes at 19psi at your altitude instead of the standard 25 @ 15.


--------------------

My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1

Edited by Professor X (05/25/19 03:41 PM)

Extras: Filter Print Post Top
Re: I seriously don't get it. [Re: Professor X]
    #26013015 -

One piece of mp tape on those lids is a fail.  If your spawn jar lids are set up the same way, they’re no good either.


--------------------
🅂🄸🄶🄼🄰


Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow

Extras: Filter Print Post Top
Re: I seriously don't get it. [Re: Mycolorado]
    #26013080 -

Mycolorado said:
One piece of mp tape on those lids is a fail.  If your spawn jar lids are set up the same way, they’re no good either.


True. Micropore has huge un-micro pores. I wouldn't trust it, but I hear it works for other peeps with more layers on grain jars...


--------------------
First Grow

Extras: Filter Print Post Top
Re: I seriously don't get it. [Re: Raccoon]
    #26013097 -

What kind of substraight are u using? U said ur friend had 7 good grows and u just got mold when u did it the exact same way. Maybe your sub is too wet :shrug: That can cause mold as well. But I also agree mold normally means u didn't have clean spawn to begin with. Just something to think about.


--------------------
The thing about deceit is, you don't know your being deceived...

The only thing I am sure about is that I know nothing - socrates

Believe nothing you hear and half of what u see.

Extras: Filter Print Post Top
Re: I seriously don't get it. [Re: Raccoon]
    #26013099 -

I use single layer over 1/4" holes, never been a problem. I've never had a failure due to filters. I 100% assure you OP has trich hiding on his agar.


--------------------

My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1

Extras: Filter Print Post Top
Re: I seriously don't get it. [Re: Professor X]
    #26013111 -

What's the pore size on micropore tape? They don't say because it's not actually a micropore. See this thread. Seems like luck no contams get in. OP, I'd use more layers or a SFD just until you figure out your issue. Then you can go back to micropore tape to see if it contams again.


--------------------
First Grow

Edited by Raccoon (05/25/19 05:40 PM)

Extras: Filter Print Post Top
Re: I seriously don't get it. [Re: Professor X]
    #26013116 -

Like a lot of your advice on here, that’s shit. :shrug:  “Micropore” simply means you can’t see the pores with the naked eye.  3M makes no claim of it stopping microbes or other small particles.  If you’re going to try to use it as an effective filter, multiple layers should be used.  A single piece is no good.


--------------------
🅂🄸🄶🄼🄰


Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow

Extras: Filter Print Post Top
Re: I seriously don't get it. [Re: Mycolorado]
    #26013120 -

The pores in micropore tape are bigger than cube spores which are pretty big


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

Extras: Filter Print Post Top
Re: I seriously don't get it. [Re: bodhisatta]
    #26013166 -

I'm really sorry, you guys are right, it must be his shitty filters that are wrecking everything he does for the last 7 attempts and not his culture that he has transferred 4 times in massive chunks from mush zombie jar to mush zombie jar. That has to be the culprit, damn micropore tape.

I use polyfil low loft batting for all of my filters by the way. I have used micropore in the past with no issues simply to debunk the myth that contaminants are sneaky little ninjas. It's an effective filter, I found that multiple layers restrict ge and cause slow progress. This is once again only my experience, maybe you have actual mutant ninja mold where you live.


--------------------

My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1

Extras: Filter Print Post Top
Re: I seriously don't get it. [Re: Professor X]
    #26013179 -

I have to tell you, I love how every time I share what works for me I am accused of giving bad advice.

Here's some good advice, any advice given with the attitude that it is the only way it works is bad advice, period.

Sad thing is you guys know where he got that info and are still debating easy bs being the issue. Pastys are plastic.


--------------------

My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1

Edited by Professor X (05/25/19 06:22 PM)

Extras: Filter Print Post Top
Re: I seriously don't get it. [Re: Professor X]
    #26013190 -

stonesun said:
Well I didn't measure the the pore sizes,
instead took a piece of tape and mounted it on a slide.
Hydrated some P. cubensis spores and with an inoculation loop
mounted them on the tape.
The gaps are huge comparing to cubensis spores, which are ~12-17µm.
I circled the fibers with individual spores sticked to them.
Sorry for the kinda crappy images, didn't put a whole lotta effort in it...

100X


400X


400X



https://www.shroomery.org/forums/showflat.php/Number/13642707/fpart/1/vc/1


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

Extras: Filter Print Post Top
Re: I seriously don't get it. [Re: Professor X]
    #26013200 -

Micropore tape pores are bigger than most contams. Make your own judgment on what to use. It seems obvious to me, given that the pores are bigger than mold spores, to choose to just use a superior filter. Why not? It's probably not his issue, but fuck it, eliminate one more variable.


--------------------
First Grow

Extras: Filter Print Post Top
Re: I seriously don't get it. [Re: bodhisatta]
    #26013205 -

Also on that same thread he shows toilet paper. Not much better, you should just use toilet paper, OP.

stonesun said:
P. cubensis spores on TP.......:rofldrunk:

40X


100X


400X




--------------------
First Grow

Extras: Filter Print Post Top
Jump to top Pages: | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |  [ show all ]

Shop: Original Sensible Seeds Autoflowering Cannabis Seeds   North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   Sporeworks.EU Spores for European Microscopy

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Don't kill yourself for ignorance! ChangLee 1,990 10 01/30/02 11:34 PM
by MickyFinn
* 3M Micropore surgical tape = lid filter agar 13,826 14 06/20/05 09:36 AM
by backupwards
* Is this Micropore? d4a2n0k 2,176 14 04/01/06 12:31 PM
by mogur
* Micropore Tape distortion 4,355 13 04/21/03 01:09 AM
by CultyVader
* jars & micropore tape... bongtoke 4,390 12 02/27/06 04:54 PM
by bongtoke
* two holes better than one? (lids with micropore tape and polyfill) FGL 1,608 5 02/27/06 05:43 PM
by FGL

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
4,476 topic views. 9 members, 320 guests and 121 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.033 seconds spending 0.012 seconds on 18 queries.