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Are these LCs visibly contaminated?
    #25994942 -

I am aware that microscopy or agar would be needed to truly tell, but I just wanted some verification.

First pic is just the three at rest, rest are them swirled up






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Re: Are these LCs visibly contaminated? [Re: Severance13]
    #25994947 -

All the comments will be people throwing darts.
If they're not obviously bad they're maybe good or maybe bad

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Re: Are these LCs visibly contaminated? [Re: bodhisatta]
    #25994953 -

Makes sense. You always got the wisdom with ya, brother

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Re: Are these LCs visibly contaminated? [Re: Severance13]
    #25994979 -

What was your recipe? They "look" fine....depending on a lot of unknown variables lol.

The important question and probably the one that will lead to more of an answer is: Why are you concerned they might not be ok?

Darts Thrown!


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Question everything, it's very responsive.

culture storage

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Re: Are these LCs visibly contaminated? [Re: Nichrome]
    #25995076 -

Stick some on agar and you'll know for sure

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Re: Are these LCs visibly contaminated? [Re: UncleHoyphae]
    #25995085 -

Or a test jar. Or a whole bunch of jars if you're willing to assume the risk

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Re: Are these LCs visibly contaminated? [Re: bodhisatta]
    #25995124 -

Excluding Agar, the best way to determine if they are contaminated would be to describe your process in detail.


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.-Non sibi sed patriae-.

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Re: Are these LCs visibly contaminated? [Re: MechaLegend]
    #25995144 -

Did you use spores or agar?


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Re: Are these LCs visibly contaminated? [Re: Severance13]
    #25995220 -

If they are bad, dilute some to mostlytap water (for the chlorine). Then wait a couple months. Check back and make sure mold is growing. Grow a brf jar of it. Wait till you see the mold. Then use good lc of the same species to graft healthy mycelium over the "foreign object" and pray But use lc you know is good. Spray on top of inoculation point. I did and started my own pin mold killing machine. Granted only aborts grew from the inoculation points.

Edited by asdflkj115 (05/15/19 09:58 PM)

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Re: Are these LCs visibly contaminated? [Re: asdflkj115]
    #25995438 -

test your LC on agar before you knock up a bunch of stuff. Probably your best bet to find out if your LC is good.


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Song Of Healing
:super: Updated Pf Tek Guide :super:
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrán and it means that of which is of itself.


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