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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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glass petri dishes and PDA sterilization
    #25929563 -

Hi everyone!!
I hope you can help me: I have doubts about glass petri dishes sterilization.

Are the Petri glass plates wrapped in foil and then PC (15 minutes at 15 psi)? I read in this forum that the petri dishes have to be put inside a pp5 bottle.
The PDA also to PC (15 minutes at 15 psi)?
Can I sterilize them together? If not, how is it sterilized separately to avoid contamination?

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Re: glass petri dishes and PDA sterilization [Re: pipazzo]
    #25929581 -

Find just one tek and follow that one, as far as I know agar is mixed together and is either pressure cooked in a container then poured in a sab or flowhood, or it’s called no pour where the agar mixture is put in separate pp5 containers then pressure cooked.

Follow any of these, they are generally different methods https://www.shroomery.org/forums/showflat.php/Number/22721954/vc/1#22721954


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Edited by The lurker (04/11/19 08:33 PM)

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Re: glass petri dishes and PDA sterilization [Re: The lurker]
    #25929602 -

With glass Petri dishes I make a mix in a bottle, get it warm and mixed well, and pour in my petri's an PC for 45 min at 15psi


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:volcano: Thanks too all :volcano:
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Re: glass petri dishes and PDA sterilization [Re: XnMe]
    #25929676 -

I stack my petris upside down and then put the stack into a spawn bag (pc safe bag) and then fold the top and fasten it with a paper clip. Then I make 2% agar  in a glass bottle and pc the whole deal. I put the petris in the bag upside down si you can just flip them right side up and pull the bag off. They also make these awesome canisters for sterilizing petris https://www.boekelsci.com/petri-dish-canisters-stainless-steel.html

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Re: glass petri dishes and PDA sterilization [Re: Thesunbeam]
    #25929856 -

I oven-sterilize my petris, PC the agar in a bottle, then pour in a SAB. This makes for a lot less condensation than no-pour.


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How I do glass dishes

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Re: glass petri dishes and PDA sterilization [Re: poisoned]
    #25930356 -

im not sure yet

Are petri dishes wrapped in aluminum foil or put inside a jar?

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Re: glass petri dishes and PDA sterilization [Re: pipazzo]
    #25930575 -

Easiest way to sterilize a GLASS petri dish is to wrap it in foil, and place in a 350 degree oven for 30 minutes.


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Clinical Management of High Dose Psilocybin Sessions:
https://www.youtube.com/watch?v=FbHOTIqjZLk

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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: placas de petri de vidrio y esterilización PDA</font></font> [Re: Psuper]
    #25930585 -

Psuper said:
Easiest way to sterilize a GLASS petri dish is to wrap it in foil, and place in a 350 degree oven for 30 minutes.


hi
i know that, but i just have a PC, thats why im not sure how to prepare the glass plate for PC

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Re: <font style="vertical-align: inherit;"><font style="vertical-align: inherit;">Re: placas de petri de vidrio y esterilización PDA</font></font> [Re: pipazzo] * 2
    #25930798 -

Put them in autoclave bag, spawn bag, or foil and then sterilize them for 15m at 15psi :shrug:


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North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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