Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery


This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: MagicBag.co All-In-One Bags That Don't Suck   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Sporeworks.EU Spores for European Microscopy

Jump to first unread post Pages: 1 | 2 | 3 |  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Sterilizing Glass Petri FAIL * 1
    #25633196 -

This is my third time using my new glass Petris.  The first two times I oven sterilized and it worked well.  This time I had room in the pc so decided to foil wrap 6 per pack and put in with my grains.  All 6 are showing contams.
I also did some on plastic Petris and everyone of them are good.

How does everyone sterilize their Petris?



--------------------

Extras: Filter Print Post Top
Re: Sterilizing Glass Petri FAIL [Re: SunnyDayze]
    #25633202 -

How much did glass petries cost you? I thought about trying glass petries for no pour containers but ended up doing no pours with MEA in 125mL jars. Sterilized in PC. Is working well, minimal contams.


--------------------

Extras: Filter Print Post Top
Re: Sterilizing Glass Petri FAIL [Re: sh4d0ws]
    #25633210 -

I don’t remember exactly but think a dozen was around $60. 

Thought I would use plastic 60mm for transfers then grow out on glass 100mm.  My compromise to cut waste but when you have to oven sterilize for two hours or more it’s still wasteful, just in a different way.


--------------------

Extras: Filter Print Post Top
Re: Sterilizing Glass Petri FAIL [Re: SunnyDayze]
    #25633222 -

I have around 50 glass petris that I dont use anymore. No pour is the shit, fuck agar, too, I do straight to 125ml baby food jars using brf+verm pucks...


It feels liberating to actually nail this isolation game.


in conclusion, I suck at agar. hahaha


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..

Extras: Filter Print Post Top
Re: Sterilizing Glass Petri FAIL [Re: pablokabute]
    #25633238 -

Haha!  I love agar, love watching the mycelium grow.  Just hate throwing out all those dishes.


--------------------

Extras: Filter Print Post Top
Re: Sterilizing Glass Petri FAIL [Re: SunnyDayze] * 1
    #25633259 -




from surplus brf pucks, spawned to unwashed tote box, unpasteurized cow manure.


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..

Extras: Filter Print Post Top
Re: Sterilizing Glass Petri FAIL [Re: pablokabute]
    #25633538 -

I’m surprised you don’t have other little plants growing in there


--------------------

Extras: Filter Print Post Top
Re: Sterilizing Glass Petri FAIL [Re: SunnyDayze]
    #25633542 -

It just seems weird to me that 2 hours in the pc didn’t work.  Or was it when I brought it out to the SAB?


--------------------

Extras: Filter Print Post Top
Re: Sterilizing Glass Petri FAIL [Re: SunnyDayze]
    #25633573 -

You're assuming the problem was the Petris. It's probably your media container for the agar.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

Extras: Filter Print Post Top
Re: Sterilizing Glass Petri FAIL [Re: elasticaltiger]
    #25633634 -

:whathesaid:

I was gonna say it was probbably contam that entered when you were pouring the agar, but TC knows better.
It also could be something you do during the pc cycle (unlikely though)

Good luck man:thumbup::mushroom2:


--------------------

Extras: Filter Print Post Top
Re: Sterilizing Glass Petri FAIL [Re: elasticaltiger]
    #25633742 -

elasticaltiger said:
You're assuming the problem was the Petris. It's probably your media container for the agar.


Oh, that’s definitely possible.  I had the other plates poured already from a different media bottle. It’s jogging my memory now, when I reheated the agar, in a pot of water on the stove, I forgot to loosen the cap (slightly)and it built pressure inside.  There was a sound when I opened it that made me cringe a bit. 

That renews my hope, going to try the dishes again.

Thanks Tiger!


--------------------

Extras: Filter Print Post Top
Re: Sterilizing Glass Petri FAIL [Re: SunnyDayze]
    #25634124 -

Glass dishes are not for no pour.

You sterilize the glass dishes empty, then pour them like you would with
disposable dishes. If you sterilize them with media like no pours, then they
boil out and suck comtams into the dishes on cool down.

How did you sterilize them?


--------------------

Extras: Filter Print Post Top
Re: Sterilizing Glass Petri FAIL [Re: natedawgnow]
    #25634168 -

:whathesaid:


--------------------

Happy to help, happier to learn
Feel free to slide in my DM

To the man in a wheelchair who stole my camouflage jacket...

...you can hide but you can't run...

Trade List

Extras: Filter Print Post Top
Re: Sterilizing Glass Petri FAIL [Re: natedawgnow]
    #25634378 -

I pour agar into glass petris before PC'ing and have no issues with boil over or contamination.

Extras: Filter Print Post Top
Re: Sterilizing Glass Petri FAIL [Re: Boarders]
    #25634408 -

I have oven sterilized the EMPTY dishes but am always a bit confused on temperature and time.  I’ll go 2 hours at 375 but not confident that is right.  The last time though I tried to sterilize EMPTY (wrapped in foil) in the pc, 18lbs for 2 hours along with my oat jars. Had it on top rack.

I have only ever done poured agar.  I will make media bottles of agar ahead, pour some extra plates and and reheat pour the rest as needed.  I do also make sure to use the whole bottle and if I don’t I’ll dump the rest.  I don’t cap and reuse out of the same bottle.


--------------------

Extras: Filter Print Post Top
Re: Sterilizing Glass Petri FAIL [Re: SunnyDayze] * 1
    #25726673 -

SunnyDayze said:
How does everyone sterilize there petris?


I'm pretty new to all this a couple months ive been playing with agar and when i jumped the gun i bought 24 60mm glass petri's I've been pressure cooking them along with my media bottle of agar for 45min @ 15 ps1 in these pp5 containers stacked upside down, i leave the lid loose on the container and leave them upright in the pc, when i unload the pc i tighten the lid and invert them into my SAB for pouring, i use the container lid as a stand and the container as a sleeve, i pour and return the container to let cool. 8 fit in the large and 4 in the small nice and snug.



Of course they have gotton a soap and water bath after use as well.

Extras: Filter Print Post Top
Re: Sterilizing Glass Petri FAIL [Re: SunnyDayze]
    #25726695 -

How do you load your PC with the glass petri's?

Extras: Filter Print Post Top
Re: Sterilizing Glass Petri FAIL [Re: SunnyDayze] * 2
    #25726701 -

10m at 15psi would sterilize glass dishes. Getting them out without compromising them is where you most likely fucked up


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

Extras: Filter Print Post Top
Re: Sterilizing Glass Petri FAIL [Re: SicSixSin] * 1
    #25726715 -

I've been using the same glass petris no-pour for years. About a dollar each. No special process. Mix the agar in a small pot, pour into dishes, wrap 5 in foil, PC for 30. Never had a problem.


--------------------
It's so easy to laugh
it's so easy to hate
It takes guts to be
gentle and kind
-the smiths

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

Extras: Filter Print Post Top
Re: Sterilizing Glass Petri FAIL [Re: LadysKnight]
    #25726717 -

What pressure you PC at?

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3 |  [ show all ]

Shop: MagicBag.co All-In-One Bags That Don't Suck   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Sporeworks.EU Spores for European Microscopy

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Glass Petri Agar Tek
( 1 2 3 4 all )		 sandman420 6,503 73 12/11/23 10:40 PM
by starburstmemories
* how long to steralize glass petris in oven? Gr0wer 1,041 3 04/29/04 06:55 PM
by Gr0wer
* How is the best way to pressure cook Petri Dishes?
( 1 2 all )		 SafeHaven 19,155 20 12/09/23 10:27 PM
by starburstmemories
* Cheap Petri dishes on Ebay. RoadkillM 3,199 10 10/25/02 02:15 AM
by boxtop703
* Re: I need glass slides, testubes, and agar dishes Anonymous 1,972 9 08/20/00 09:25 PM
by Anonymous
* PETRI Incognito 593 2 02/10/02 11:43 AM
by pleezr
* petris ellisdee625 690 3 10/14/02 06:39 PM
by ellisdee625
* Re: Sterilizing With A Nukrowave Anonymous 1,489 4 12/15/99 06:42 PM
by PanTrop

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,128 topic views. 5 members, 2,396 guests and 46 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.039 seconds spending 0.013 seconds on 18 queries.