Noisecontrol said:

Incubation is not necessary, higher temps favor contams. The whole process is optimal around 75, you could do 77 for spawn without problems too. 70% alcohol is better than 91%. Those are just little things tho. Sterile technique is ultimately your best bet. Watch all contact when working in your SAB, alcohol wipe before flaming, don't suck in any air, don't touch anything but your inoculation point with the needle once it cools. That all helps but the other big thing is PCing long enough. At least 90 minutes. I typically do 120. All of these things are little pieces to one big efficiency puzzle, and once you refine your procedures you'll see better results.

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nube424 said:

@noisecontrol. Sorry for the misunderstanding. I didnt mean YOU told op to throw it out. I was just talking in general.

Last year a ton of people told me to throw out a bacterial mono. Glad i didnt cuz the first flush was like 1500g wet. Crazy. I posted it and shamed everyone that was an "expert" that told me to chuck it. Lol. So, sorry. Didnt mean u.

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RalphieRamone said:

When you harvest from a tub that has a bacterial contamination, dont you worry about any health risk when consuming the mushrooms? I'm just assuming it would make you sick.

When I think of harvesting from a contaminated tub all I get is the Pepto Bismol jingle playing in the back of my head haha.

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Noisecontrol said:

nube424 said:

@noisecontrol. Sorry for the misunderstanding. I didnt mean YOU told op to throw it out. I was just talking in general.

Last year a ton of people told me to throw out a bacterial mono. Glad i didnt cuz the first flush was like 1500g wet. Crazy. I posted it and shamed everyone that was an "expert" that told me to chuck it. Lol. So, sorry. Didnt mean u.

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No worries and that's the exact reason I keep them too, unless I need space. The only thing it hurts is colonization speed and longevity.

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Today there's been an update with 3 of the jars over night. (Amazed at the speed of change) 2 of the jars have what seem like healthy pins in them both appear like stems with a cap on the top of them with multiple other spots forming as well. Unfortunately I only have a picture of the one of the two jar that appear to be pinning well.
The jar thats in question. The sight got a little bigger with kinda a light brown tint to it I have two pictures of it.
The other jars aren't pinning yet and still have some medium to grow into which I expect to take 3 days at this point.
Keep in mind turning down the brightness helps with the glare of the jar

First two of the healthy one. Last two of the jar in question (which has a fee pins as well as the spot I'm worried about)

Caps McGee said:

Flame sterilize with 91%, use 70% on your hands...Don't flame sterilize and then wipe with alcohol... run a drop of solution out of the syringe in the SAB before innoculating... wiping with alcohol only sanitizes, and  I think RR said something like "it's like taking a shower, then wiping shit on your face"... lol, vivid expression, but it portrays an important point... alcohol also does not kill spores, and you could inadvertently introduce contaminants during inoculation ... definitely need a  SAB, or you're wasting time, money, and resources IMO... you can spawn and fruit in open air,  but this entire process depends on acquiring and introducing clean spawn...

RalphieRamone said:

When you harvest from a tub that has a bacterial contamination, dont you worry about any health risk when consuming the mushrooms? I'm just assuming it would make you sick.

When I think of harvesting from a contaminated tub all I get is the Pepto Bismol jingle playing in the back of my head haha.

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You can make tea with questionable fruit

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Noisecontrol said:

Nah it doesn't "just happen" that's bad info and gives people the idea that inadequate procedure will produce impressive results. No disrespect just need to be careful what we share here as the novice is impressionable until he/she learns from their own experiences. Those jars are likely bacterial, that's why they've pinned before fully colonizing. When properly hydrated and sterilized your jars would be ready in half that time. The error would be in your grain prep, likely not sterilized long enough. You probably soaked your grains ya?

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Corundum said:

Noisecontrol said:

Nah it doesn't "just happen" that's bad info and gives people the idea that inadequate procedure will produce impressive results. No disrespect just need to be careful what we share here as the novice is impressionable until he/she learns from their own experiences. Those jars are likely bacterial, that's why they've pinned before fully colonizing. When properly hydrated and sterilized your jars would be ready in half that time. The error would be in your grain prep, likely not sterilized long enough. You probably soaked your grains ya?

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If you have found a way to go from spore to fully colonized cake in 10 days I would love it if you could share this with us

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