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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Slurry Spawn or Liquid Inoculant [Re: tombosley8]
    #20563688 -

1 pf jar was used to slurry inoculate 39 quart jars of WBS grain and 1 spawn bag containing enough for 1 Quart jar Grain.

This was the day of inoculation: It took longer to shake these jars for 20 seconds each than to inoculate all 40.



Here is the shot 24 hours later: some close ups also



you can see it starting to spread. by day 2 it will jump from about 30-90%. two of those random jars in the mix had the leftovers of the liquid in the jar poured in. im assuming i over poured and i will toss the two out. but who knows, they may just colonize even faster. i guess we shall see tomorrow.


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EAT GETS SHIT DONE


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Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
    #20563721 -

So I'm sold, but to explain it all. I go MS-agar-LI-PF-slurry grain-tub. I use clean agar from prints to make a LI, inoculate PF jars (5days), then use those in my grain via slurry. I don't have a time frame for colonization times yet from the slurry. But  I have to say this is the best tek for fast colonization. But, you have to have the right material.

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Re: Slurry Spawn or Liquid Inoculant [Re: Sockadin]
    #20563819 -

yes you can do that. i went straight from spore syringe i made to pf jar. 19 days, then let it sit about 5 more days. i stuck the pf jar in the fridge for about a few weeks until the quarts were ready to slurry. took out the pf jar then slurried. ive done it this way using multispore with no issues every time ive tried it. ive also done similar to what you just said. i went from stem tissue LI-Pf jar-slurry grain-bulk. once you get good clone candidates on a flush, its super quick to go from clone to fruit using this. and make a ton of spawn within a matter of a week. ill show you very soon with more detail.


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EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

Edited by eatyualive (09/14/14 12:18 AM)

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Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
    #20564146 -

:rockon: thats fucking killer stuff eats!


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Slurry Spawn or Liquid Inoculant [Re: cronicr]
    #20567326 -

Here is the 48 hour shot. i tried to keep the same angles.



whats so awesome about this, is that instead of doing g2g 5 different times with 5 different grain jars, or agar wedges ect. i can get enough spawn colonized from 1 pf jar in one sitting. it will be ready in less than a week. so the potential to have 10 monotubs fruiting from 1 pf cake in 2 weeks is pretty astounding.

then what you do is save 10 of those quart jars for your masters if you want to g2g from that. or you can just do slurry the whole time. pf jars take up less space in the fridge. i only do about 25 pf jars a year. that lasts more than a year. 5 jars of each strain so you can see where this is super easy to maintain a constant rotation with different strains. combine this with lets say something like agar's GLC or fahtsters dried grain cloning and just holy shit you have a fantastic library of strains available at all times on a consistent timed basis. so your looking at fruit from 2 weeks after jar inoculation.

also i was doing p2g for the last few years due to i was only doing small batches. but if you really want to do a full scale rotation with 200 quarts a week. that can be accomplished with 2 pf cakes.

fanfuckingtastic.:mushroom2: im not really sure why i even started doing p2g or g2g anymore. i think im going to stick with this again for a while. its easy to do some big test batches of each strain all at once.

Super RAD

Edited by eatyualive (09/14/14 08:24 PM)

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Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
    #20567541 -

did i miss all the tubs that all this spawn is making :shrug:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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Re: Slurry Spawn or Liquid Inoculant [Re: tripdawg420]
    #20567587 -

I cant seem to find a blender that fits nicely on a half pint jar lid. Best thing I could find would be a MagicBullet. The TV blender that each cup can blend then be screwed on and off. Could this work as well?

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Re: Slurry Spawn or Liquid Inoculant [Re: motherchimp]
    #20567615 -

no ive done a bunch of grows in the past with spawn prepped this way. but im going to post this next batch to show.

but you can use 1 pf cake to make 100 quarts grain jar spawn. enough spawn for 20 tubs if your using 5 quarts a tub. all done using 1 pf cake.

this thread is just illustrating the method. but once i get the updates ill post in the original post.


motherchimp said:
I cant seem to find a blender that fits nicely on a half pint jar lid. Best thing I could find would be a MagicBullet. The TV blender that each cup can blend then be screwed on and off. Could this work as well?


try an oster blender. walmart and target have them.


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

Edited by eatyualive (09/14/14 08:22 PM)

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Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
    #20567788 -

Ah so my best bet is to buy this: http://www.ebay.com.au/itm/Free-ship-Blender-Ice-Crushing-Blade-6-knife-Replacement-For-Oster-Kitchen-4980-/191228347945?pt=AU_Utensils&hash=item2c86190629&_uhb=1

Walmart doesnt exist where I live.

Edit: Wait just realised I need the whole blender.......... :thousandisland:

Edited by motherchimp (09/14/14 08:58 PM)

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Re: Slurry Spawn or Liquid Inoculant [Re: motherchimp]
    #20567893 -

yes but you need the blender base also. this is a good site.

http://www.goodmans.net/d/1377/oster-blender-blades.htm

the vintage ones have a metal blade assembly you can pressure cook. the black plastic assembly can't be pced it will warp. but that part is only touching the outside of the glass so it hasn't ever caused me any problems.



http://www.ebay.com.au/itm/Vintage-Osterizer-Square-Glass-Blender-Jar-Metal-Base-Blade-Gasket-and-Lid-/231311554334?pt=Small_Kitchen_Appliances_US&hash=item35db3e371e


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

Edited by eatyualive (09/14/14 09:10 PM)

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Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
    #20568079 -

Find metal base blender attachments. Find them in ebay.


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My Perfect wheat preparation TEK
My holy grail no pour agar tek

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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: Mr. Alien]
    #20571724 -

updated op

72 Hours:



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EAT GETS SHIT DONE


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Re: Slurry Spawn or Liquid Inoculant [Re: Mr. Alien]
    #20571732 -

Mr. Alien said:
Find metal base blender attachments. Find them in ebay.


the metal base is a useful tool if you have the extra money to spare. but the plastic black piece works perfectly fine if you wipe it with bleach wipes ect. it only touches the outside of the glass and never touches the inside of your liquid inoculant. i actually use my attachment two times to 3 times in one sitting. i move it from one jar to the next to quickly blend the liquid inoculant.

if you use quick fast and precise motions, minimize your airtime its extremely easy. if you can do agar work, then this is half as hard as agar work.


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

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Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
    #20571846 -

I just switched to regular mouth pints from half pints and picked up a second blender(an old oster) now I can inoculate four times as many jars at a time.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     

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Re: Slurry Spawn or Liquid Inoculant [Re: MudaFuka]
    #20571890 -

yeah i use both. i use the 1/2 pints for my 9er tek cloning which im trying to do today or tomorrow. and then i use the pints for the slurry. i only need the half pint for clones because i can easily do 10 LI syringes or 12 from 1/2 pint blended stem. then i could shoot a little into agar or pf jars.

im going to attempt both soon. since i don't have the pf jars ready yet, im going to clone the stem tissue and suck into syringes. then inject them into pf jars the next day. i also want to save about 1cc to shoot into agar. i just won't be able to make the agar until 2 days from now. so coming soon...

i actually have just about every type of jar there is. lol,  but i do have quite a few extra regular mouth pints that i use to drink out of bc i only use a few for Liquid Inoculants.

it sux i want to clone but timing never works out for agar. gonna have to be on the next flushes.

pints are definitely helpful!


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

Edited by eatyualive (09/15/14 08:15 PM)

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Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
    #20578880 -

Does it have to be a PF cake or could you theoretically take a fully colonized wbs jar and make a slurry from that ? It seems to me that the benefit comes from a wide spread, hydrated, inoculate that instantly feeds on as much substrate as possible right from the get go.


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Re: Slurry Spawn or Liquid Inoculant [Re: vertygo]
    #20580339 -

no you don't want to blend the grains into pieces. exposing the inside uncolonized portion of the grain would cause some issues. now, that being said, i have not tried it. but if i were to try it, id probably only slurry one jar just to test it out. i think it would probably be a quick way of farming trich.


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

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Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
    #20589632 -

ok so here are the F plus tubs that were slurried beginning to pin.

NO DIRTY SPAWN, NO CONTAMS BEING SWIRLED AROUND IN A SHMUVBOX. THIS CONTRADICTS EVERYTHING EVERYONE WAS SAYING AND GIVING ME A TON OF BACKLASH!

IT WORKS! I already knew this for years but just had to show everyone because of all the doubters.



--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

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Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
    #20589640 -

:thumbup: You have me convinced. Good stuff.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     

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Re: Slurry Spawn or Liquid Inoculant [Re: MudaFuka]
    #20589654 -

:congrats:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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Shop: Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   Sporeworks.EU Spores for European Microscopy   MagicBag.co Certified Organic All-In-One Grow Bags   North Spore Bulk Substrate

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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