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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive] * 1
    #20489967 -

So here's my first attempt and am very satisfied with the extreme growth rate. I inoculated Late on 8/21
Post from 8/24:
tombosley8 said:

so here's a couple i slurry tek inoc.'d on 8/21(late). i shook them today but I can't tell if the extra amount of slurry i used in these jars will cause bacteria (can't see any pooling:shrug:) but at least i can see the true power of the slurry. AMAZING:eek:  I also used jars that had been sitting around after pcing for 3 weeks so i wasn't expecting anything except a first try at it. Now that i have that first experience the next should be that much easier. I have a few more jars that i didn't dump from this session that are probably about half as colonised because i used probably about half as much slurry. I used too much liquid for half the cake(1/2 qt water) Also this cake was inoculated with a wedge i am not 100% sure about so the future ones will be much more reliable as far as results. Doing up the real attempt tommorrow with fresh jars and a clean cake.


these responses regarding
eatyualive said:
i also updated the original post to explain my lower water content pf mix specifically for liquid cloning and p2g transfers. the dryer mix also helps the p2g because it allows the pf cake to break up into smaller pieces for transfer.


eatyualive said:
tombosley8 said:
you are awesome. ^^^^that's the info I needed!  What do you think is the best way to colonise a cake the fastest? LC? If  I make the cake a lot dryer can i dump a load of lc all over it?


my recipe for 24 pf jars
12 cups verm
6 cups brf
5.5 cups water. i use a half cup less so the pf jars are a little dryer. i intentionally do this for liquid culture injections.

regular 24 pf jar recipe
12 cups verm
6 cups brf
6 cups water

this post shows some close ups of my mix. and a description of how i add that last bit of water to the mix.

http://www.shroomery.org/forums/showflat.php/Number/20300997#20300997

here is another post i made with some better close ups of the wet mix after i blend it with a hand mixer.

http://www.shroomery.org/forums/showflat.php/Number/20428297#20428297

ill then use a syringe full of lc and inject one whole or a half syringe into 1 pf jar. this grows very fast with liquid cultures in a few days and then i can g2g that or blend it up and make a ton of jars with a slurry. you may even find that if your going to add a whole syringe to the pf jar, you may want to add 1 cup less than the 6 cups total water for the 24 pf jars. just divide the formula by 24 to get the individual jar ratio.

but if you intend to try the slurry. id inject your lc with a sterile syringe into a pf jar. i usually use a sterile syringe and suck up the liquid culture into it, then inject into a pf jar. im always hesitant adding more moisture to grain jars. but lc's are quick and usually colonize the jars rather quickly. but pf jars are a safe bet. and also, if your only injecting 2 pf jars. your not contaminating 10 quarts if your using a dirty lc. so pf jars for me are also a way of testing if something is clean or not.



and today's update:
tombosley8 said:
slurry works magical wonders. This is the update:
the two on the left were the shaken in comparison with the three to the right that were not:
and some side by sides with the shaken one on the left and non-shaken on the right:
so the ones that i shook were ahead of the others and now after a shake are probobly right in line with the others (or less:rolleyes:) well anyways lesson learned again thanks i did a slightly weak slurry with a trusted cake/culture on the 25th but it looks like i should made it stronger. I c2gd a couple jars from that cake and should have just slurried it. Anyway i got some cakes (still regular mix) noc'd up with a bunch of lc and they are blowing up and i just pc'd a bunch more cakes at you recommended recipe and did some even dryer just to see how much lc i can hit em with. This is so much fun watching the rapid growth and very gratifying (almost instant). I urge other cultivators to give the slurry a try it has changed everything for me
so for these jars above i am going to test a jar in some bulk to see if the cake was contammed or not but as far as slurry performanceI have no issues yet and i thought that these were fails off the bat because i used so much liquid in the slurry. Really surprised me


and response with info:
eatyualive said:
yes you will notice that the p2g and g2g take much longer to establish a foothold onto the media. while the slurry is almost instantaneous.
i feel that it wasn't a bad thing that you shook the jars, but it wasn't completely necessary. and thats a thing people can decide to do for themselves. like option a or option b.


I'll take the red pill  :blueorred:
thanks  :feelingfab:


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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: tombosley8]
    #20489978 -

Yes im going to take the red pill from now on as well!

How to Colonize Quart jars in 3 Days


hell it colonized a bulk substrate with 1/2 pint of mycelia spawn to 16 quarts of substrate volume. it went from bulk inoculation to fruit in 18 days.


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Edited by eatyualive (12/04/14 10:40 PM)

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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
    #20491012 -

I'm trying it ASAP:crazy2::thumbup:


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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: tombosley8]
    #20491296 -

yeah i mean using one pf cake made the yield a little low. but maybe you can use a little bit more spawn ratio. like 2 pf cakes lol.


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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
    #20491643 -

here's this too:
tombosley8 said:

and after pcing:
and still working   so i'm satisfied
they had replacement blender attachments and gaskets for $10 at walmart BTW

but gluing was difficult so to anyone get muda's blender and all is very easy(and the base is PCable(they don't make that model so don't order that). I would want a wide mouth attachment for the osters (FOR TIGER DROP) but a wide mouth doesn't fit in my blender so i'm waiting for that til i get the blender muda recommended. Thanks guys I'm doing my debut LI tommorrow with better knowledge (less liquid) and a now easy to remove lid (NO FLOPPING GASKET)


from this thread http://www.shroomery.org/forums/showflat.php/Number/20429745/fpart/1/vc/1


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Edited by tombosley8 (08/29/14 07:07 PM)

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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: tombosley8]
    #20491868 -

yes thanks. thats a great addition to using the blender assembly. makes things more manageable and less things falling on your glovebox or shmuvbox floor.


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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: eatyualive]
    #20494265 -

tombosley8 said:
ok so i got the blender (MUDAFUKA) recommended from that link and it turns out they have remade that model with a plastic blender jar and no screw off blender attachment :mad:. So hopefully Amazon will let me return it for showing the wrong picture of the item. (FALSE ADVERTISEMENT!) So At least i got my osters i just was excited to be able to use a wide mouth jar and tiger drop that bitch. I'll Find my match soon.


bottom lined is don't get the blender muda linked to... the oster's work great once you get the gasket attached.


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Re: Liquid spawn: Slurry In a Hurry Spawn: Without the spawn [Re: tombosley8]
    #20494312 -

tombosley8 said:
tombosley8 said:
ok so i got the blender (MUDAFUKA) recommended from that link and it turns out they have remade that model with a plastic blender jar and no screw off blender attachment :mad:. So hopefully Amazon will let me return it for showing the wrong picture of the item. (FALSE ADVERTISEMENT!) So At least i got my osters i just was excited to be able to use a wide mouth jar and tiger drop that bitch. I'll Find my match soon.


bottom lined is don't get the blender muda linked to... the oster's work great once you get the gasket attached.


a friend was telling me that they actually don't even use the black assembly. they simply hold the jar on the blender. i can see slurry shooting everywhere if your not paying attention lol. ive actually had one that wasn't fully attached and it made a little bit of a mess. but its all contained in a plastic bag so it doesn't matter. it still worked as inoculant.


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Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
    #20506610 -

Auto-Summary
Liquid Spawning or Inoculant 2014, developed by TV Casualty, is a revolutionary technique for colonizing quart jars in just 3 days. The method involves creating a slurry using a small mouth pint jar, a pressure cooker, and a blender. The slurry is then used to inoculate multiple jars or bags of substrate. Key points include:

- Results: 3-day colonization of quart jars
- Materials: pint jar, band, foil, blender base, pressure cooked butter knife, black plastic base, colonized jars
- Procedure: Prepare utensils, sterilize jars, sterilize and set up work area, blend cake into slurry, pour slurry into jars/bags
- Tips: Use 1/3 to 1/2 of the pint jar for slurry, avoid shaking jars, fully colonized jars/bags in 3 days without shaking

updated op for clarity and showing results up front so you can see how lightning fast this is.

Liquid Spawning or Inoculant 2014:

First of all, TV Casualty is the man who invented this. This has revolutionized mushroom cultivation and everyone needs to know that you can successfully have fully colonized quart jars in 3 days.

TV Casualty's Slurry In a Hurry Tek

Results: 3 Days to colonize quart jars. Tv Casualty's results



tombosley8 results: Thanks tom for ignoring the haters and trying something new.



My results: but didn't take picture of the 3rd day. Ive tried this over 20 times with great successful fruiting of substrates used with the same spawn jars.




Bag Spawn after 3 days:



PROCEDURE FOR JAR INOCULANT


Materials:
  • small mouth pint jar
  • band for the small mouth jar
  • foil
  • oster blender base and blade assembly
  • pressure cooked butter knife wrapped in foil
  • black plastic base for the blender attachment
  • one colonized 1/2 pint pf jar
  • as many colonized grain jars as you want up to 100: wbs, rye berry, popcorn
  • optional: you can also use this on grain prepared in filter patch bags
  • pressure cooked foil large enough to set a pf cake on. this is wrapped in foil

Reasoning:

Usually this method works well when you have about 20-100 quart jars you want to colonize in 3 days.  If you are only doing 10 quart jars then you wouldn't need to use about 90% of the slurry because you only need a small amount per jar. I will then use another method like P2G for fewer jars. However, if your using a smaller quart jar number you can easily add less water to the mix and make the slurry more muddy. This will reduce the risk of adding too much water to each jar. You only need a small amount per jar. I would compare this to the equivalent of about a tablespoon of slurry per jar. You also need to be careful that if you add too little of water, the cake won't properly blend in the jar. I would say 1/3 to one half of the pint jar is a good ratio. 1/3 on the muddy side, 1/2 on the more liquid side with more jars.

Procedure:

1. Prepare your utensils in the pressure cooker. I wrap a piece of foil large enough for me to flip a pf cake upside down on and cut it in half. I then wrap that piece of foil with foil and pressure cook it with other utensils.

2. As you can see the small mouth pint fits snugly to the bottom of the blade assembly. Fill the pint jar halfway with distilled water and place the blade assembly and ring on top of the assembly. Twist the ring so it is tighter but not too tight. Then place foil over that. Now it is ready for the pressure cooker. Some people have issues with the plastic grey gasket that hangs on the blade assembly. Mine is so old that it pretty much sticks to the blade assembly and i don't have to worry. However, you can also RTV silicone this directly to the lid in order to make things easier. You want to make sure that the bottom part that is touching the jar is not siliconed only the top attaching to the blade assembly.




3. Place your butter knife wrapped in foil along with your other utensils(pint jar with assembly) in the pressure cooker. Pressure cook for 30 minutes at 15psi. Let it cool. Then you can begin doing the clean work to do the liquid transfer.

4. Now that you have all your utensils sterilized and your grain jars prepared. It is now time to setup your clean work area. In my case I use a
Shmuvbox or you can use a floowhood or SAB. I setup all my jars to the far right out of the way so I have room to place all my utensils up front in the shmuv. This is so I can easily reach the utensils. I wiped all the jars down with rubbing alcohol prior to sticking them inside the clean work area. You can see my foil wrapped foil in the middle, my foil wrapped butter knife in the middle and the fully colonized pf half pint on the right. You will also notice the black assembly that attaches to the blade assembly for the base of the oster blender. I wiped this down with a bleach wipe prior to putting it in the shmuv.



5. You also want to setup your blender close by. I wipe the whole thing down from top to bottom with bleach wipes and even spray it with lysol.



6. Now you can prepare everything to ensure a quick transfer. This process involves taking off the foil and band lids to the pint jar or jars. You will also take the lid and foil off of your colonized pf jar. I place the items to the side out of my working area. But you can also take them out of the shmuv completely. Although if you remove your hands, always clean them thoroughly before placing them back in.



7. Now I am about to dump the pf cake upside down on my sterile foil.



8. Unwrap your butter knife and cut the pf cake in half. Now I am doing this because im trying a less slurry or muddy mix to see if it still works. You can simply use one pf cake and dump it upside down into the pint jar. This is a technique view of the 1/2 pint jar flipped upside down on the pint jar so that the cake slides right into the jar without touching anything. If you have to move the blade assembly be sure to set it face up not touching anything on the blade. You can hold it in your hand and flip the jar at the same time but you need to be careful not to touch it on anything. You don't want to introduce any contaminants into the jar.



9. Now if your using one pf cake for less jars. Simply flip the jar upside down shown in the previous step. Or if your making multiple jars for liquid spawn or liquid inoculant you can use your sterile knife to cut the pf cake in half. You have placed this pf cake on the sterile foil which you just unfolded. Work clean and fast. You then spear the side of the cake with the knife, open up the blade assembly touching only the outside and place the cake into the jar. You can position the cake so that you can simply use the cake edge against the inside edge of the jar to use as leverage to pull the knife out and the cake will drop right in. This takes about 3 seconds max.



10. Now I place my black blender attachment assembly on the top of the metal blade assembly. I clean the area so that when I am about to do the transfers, there is clear room to work clean and fast.



11. I now take the jar and assembly out of the shmuvbox but still in my cleaned work area and use the blender to blend the cake into slurry.



12. Now the slurry is ready to pour into each Jar or bag.

13. You simply bring your jar back into your work area. You have all your quart jars and bags with loose lids ready for transfer.

14. With one hand slightly lift up the blade assembly lid with your fore finger and you will then open the quart jar lid with your right hand. You pour about 1 tablespoon of slurry into each jar. The key is to quickly close both lids simultaneously after you do the quick transfer. This minimizes contamination.

15. After you have inoculated all of your jars and bags. You simply shake the jars for 20 seconds each. You want to distribute that extra water you poured into your grain jars as much as possible so that you don't have any issues after this. At first you will get a little scared because your jars will look like there is pooling water at the bottom once they have sat on the shelf a few hours. Be patient, wait it out. The idea is to have fully colonized jars in 3 days without a shake and it works.

FOR THE SLURRY IN A HURRY TEK DO NOT SHAKE THE JARS. THE KEY HERE IS TO HAVE FULLY COLONIZED JARS/BAG SPAWN IN 3 DAYS WITHOUT SHAKING THE JARS AT ALL.
!!!!


PROCEDURE FOR LIQUID SPAWNING BULK SUBSTRATES


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Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
    #20506715 -

Much respect. I'm not a huge fan of BRF, but this is some cool stuff. Props to you and definite props to TVCasualty.

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Re: Slurry Spawn or Liquid Inoculant [Re: Nos-hroom]
    #20506722 -

yeah props to tv. im just the messenger. also his way of making the lid attachment is much cleaner. i do it ghetto style by pouring and it works well. i didn't feel like building the lid dispenser at the time i wanted to try it and it worked fine.


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Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
    #20520194 -

updating op with 77 hours growth with a light slurry. so ill be going back to this method from now on. it takes a minimal amount of extra effor to pressure cook a jar of water and a knife. and it saves you half the time.



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Edited by eatyualive (09/04/14 11:27 PM)

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Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
    #20520242 -

updating op with a friends results. day 3-5 bag spawn with slurry. also this is a burma strain print from the 7 year old clone i had.



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Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
    #20521317 -

update 82 hours.


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Edited by eatyualive (09/04/14 11:28 PM)

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Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
    #20525070 -

updating original post with spawn bag, 89 hours, 101 hours and bottom shot



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Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
    #20525441 -

This is so cool.

I'm doing my first grow right now (2 sets of grain spawn, one has 6 jars and the other has 5) but if I ever wanted to do a massive grow I would probably try this out.

I think it's better to know if you have contams in 3 days and take a little bit more of a risk than to wait 3-4 weeks and still risk contamination.


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Re: Slurry Spawn or Liquid Inoculant [Re: moonsphere]
    #20525571 -

moonsphere said:
This is so cool.

I'm doing my first grow right now (2 sets of grain spawn, one has 6 jars and the other has 5) but if I ever wanted to do a massive grow I would probably try this out.

I think it's better to know if you have contams in 3 days and take a little bit more of a risk than to wait 3-4 weeks and still risk contamination.


:thumbup:


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Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
    #20525943 -

This is the shit btw, I am now set on giving it a go. I still get a nostalgic kick out of making PF jars. This is a great way to put them to amazing use. Nice write up as always eatualive. I love how all your pics are in the shmuv box. Hit me up with a pm.

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Re: Slurry Spawn or Liquid Inoculant [Re: Sockadin]
    #20532757 -

so i just spawned two tubs with the slurried grain. the grain jars were actually more consolidated than my g2g and p2g jars side by side. the g2g and p2g had been sitting an extra week before the slurry jars were colonized completely. so in a matter of a day and a half the slurry jars were more dense by far than the other two methods. i also did spawning sessions using all 3 methods of grain colonization. slurry, pg2, g2g. and slurry by far owns the shit!


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Re: Slurry Spawn or Liquid Inoculant [Re: eatyualive]
    #20533897 -

Fo Real I have basically converted my entire method toward this because there's nothing comparable as far as speed. Although I've only seen one flush from this method the upcoming has caught up and passed up all my earlier projects(via agar wedge and g2g) and will be seeing their results soon:tongue2:. I have colonised some cakes via LC in a week thanks to your recommendation of the slightly dryer cakes. So hopefuly i can get a good clean method with agar to LC because i never thought a cake coulkd ever colonise so fast. This is amazing!!! So i'm going to be going straight from slurry to bulk as well to see how it goes. I cannot wait but i must prepare for this explosion of growth...................  :shockingsurprise:


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