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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Possible to Revive Dried Mycelium from Rye Cake?
    #19349880 -

Hello,

Let's say a person neglected a fully colonized jar off rye for six months and the spawn shrunk to about 2/3 of it's original size.  Could injecting sterile tap water into the jar and then sucking it back out into a syringe result in having a viable and living grain LC solution or is the mycelium 'dead.'  If it isn't dead, is there a certain time period one would need for the myc to 'regrow' before it is useable?

Thanks.

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Re: Possible to Revive Dried Mycelium from Rye Cake? [Re: EastBayRay]
    #19349939 -

I'm pretty sure it is possible to revive dried mycelium, I've heard of people taking dried colonized kernals of grain and placed them onto agar with success and even used the dried kernals for long term storage like slants would be used for. Not sure about making a GLC from it but you'd probably want to let it rehydrate a bit first before you'd be able to suck much of the tissue up into the syringe.


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Re: Possible to Revive Dried Mycelium from Rye Cake? [Re: mushmagic]
    #19349951 -

It's a horrible way to start a LC, take a grain out put it to agar then put the agar to LC if you must have LC.


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Re: Possible to Revive Dried Mycelium from Rye Cake? [Re: bodhisatta]
    #19349968 -

:whathesaid: but I's skip the GLC and just go with agar.


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Re: Possible to Revive Dried Mycelium from Rye Cake? [Re: mushmagic]
    #19349974 -

Quote:
Could injecting sterile tap water into the jar and then sucking it back out into a syringe result in having a viable and living grain LC solution or is the mycelium 'dead.'


No to both.  You won't get a quality liquid culture nor is the mycelium dead.

Spawn it to bulk or bury it in a garden spot outdoors and hope for the best.  For expanding mycelium you should always use fresh cell lines.
RR


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Re: Possible to Revive Dried Mycelium from Rye Cake? [Re: RogerRabbit]
    #19350632 -

Nice. Over a year ago I let some rye berry and wbs cakes in 1/2 pint jars dry out to try some experiments with reviving them at some point in the future. Not sure when I'll get around to that but I'm glad to hear that it's possible to revive and spawn dried mycelium.


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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