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Need an easy liquid tek that works
    #5149476 -

I have the whole BRF tek down as good as it gets and am getting great flushes, but I need to go semi-bulk. I keep trying to do liquid myc. teks and don't get anythign. I tried a karo tek and got good liquid myc. growth but when I nocked up some rye jars (prepared properly) nothing happened. I tried a tek where you blend up a fresh mushie to create a spawn slurry and again nocked up some rye jars and nothing happened. So can someone either suggest me a pretty easy liquid tek, I'm open to anything (I've got cash to invest if it's not too much and am an alright mycologist, just no agar please), or just give me a recipe to use. I really need to get somethign going. I just got a bunch of spores in the mail, so I have fresh syringes to use too. Please. Thank Shroomery. Peacefulness to you all.


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Load Universe into cannon. Aim at brain. Fire.

~Nailing Bee to the Honey

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Re: Need an easy liquid tek that works [Re: Synaethesia]
    #5149565 -


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Re: Need an easy liquid tek that works [Re: fufungi]
    #5149728 -

1:10 pale honey to water. heat just before boil for say 30 mins. dump in spores in a GB. let sit. i never measure properly and i never bother timing the heating and its worked everytime. when u get some germination after a few days some people drop in a little h2o2 to keep out contams. some people do some people don't. sometime i do sometimes i don't. anyhoo ive never had problems with LC so im pretty unscientific about it all.


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im sorry about your mother. She was a terrible attrative woman.

Get back to nature; hunting burgers and gathering fries.

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Re: Need an easy liquid tek that works [Re: skeletor]
    #5149951 -

This isn't a liquid tek, but it may serve your purposes.

Knock up your jars. In the meantime, buy some empty myco bags and fill halfway to the filter patch with WBS then PC. Just use a twist tie to close the bags. When you jars are colonized open up the bag and knock the jar into the bag. Mush up the spawn into the WBS. Mush it good and mix it around from the outside of the bag. Just squeeze it with your fingers. Put bag into incubator. This will 4x to 8x your spawn.

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Re: Need an easy liquid tek that works [Re: Synaethesia]
    #5150047 -

StoneInFocus said:
I tried a karo tek and got good liquid myc. growth but when I nocked up some rye jars (prepared properly) nothing happened. I tried a tek where you blend up a fresh mushie to create a spawn slurry and again nocked up some rye jars and nothing happened.



maybe the problem is with your growing substrate?

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Re: Need an easy liquid tek that works [Re: Synaethesia]
    #5150163 -


Rig LC lids like this.


1 each level tablespoon light dry malt & dry dextrose.
To a quart of water.
Add nutes to boiling water, decant through double coffee filters.
Half each into 2 quart jars.
Add a marble to each jar as an agitating device.
PC 20 minutes.
Coll overnight.

Inoculate LC with spores, colonized agar or tissue.
Incubate 76 to 84F.
agitate daily.

Wait until you have a thick LC.


Soak WBS 24 hours, rinse, drain & jar up.


PC jars 90 minutes @ 15 to 18 psi.
Shake each jar, as you remove from PC.
Coll jars overnight.

Inoculate WBS jars with LC, 4 to 5 ml each.

Keep needle wrapped in alc soaked gauze pad, at all time.
Minimizes needle exposure to open air.

Incubate jars close to 80F.



Find big block of COIR.


Buy several GLAD OVEN-WARE CONTAINERS.


Find/buy a roll of micropore paper tape.
(any drug store in bandage section)


Hydrate Coir with hot water to saturation point.
Doesn't drip when you pick up a handful.
Does drip when you give it a firm gentle squeeze.
Place coir in plastic bags.
Nuke several times for 3 minutes each.
Seal bags with a rubber band.
Allow bags to cool overnight.

Drill a couple 1/2 inch holes in GLAD OVEN WARE LIDS.
Cover holes with Micropore tape.

Use colonized WBS as spawn @ 50% rate to Coir in Glad trays.
Add lid to tray.

Incubate trays at 80F.


Once trays are colonized.
Open lid & add thin layer of casing cover mixture.
50/50 pH adjusted peat (adjusted pH 7.5)& Verm.
Add oyster shell flour as a long term buffer.

Incubate trays 3 to 5 more days.
(until myc hits casing surface in spots)


Remove lids.
Place trays in a fruiting chamber.
(85 to 95% rh & 80F)
Expose to 12/12 light & FAE.





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Re: Need an easy liquid tek that works [Re: agar]
    #5150731 -

WoW thanks for the replies, I just got a BRAND NEW 22 qt. PC and I'm anxious to break it in so I'll probably be doing a lot of work this weekend. Going to try a couple of these teks, but Agar's interest me the most. I think I tried your karo tek agar before and just couldn't get it to work, but this one looks more promising. Just some mat. questiosns:

For those quart jars, where did you get the lids? They look like peanut butter jar lids almost, but I've never seen lids like that before.

Is that rubber tubing (like what you use to tie up with) or a small gauge copper pipe...referring to the tubes for air exchange in the LC jars. How far does the tubing extend into the jars, all the way into the solution or just barely in, and then, is that polyfill in the ends of the tubes or something elese?

I live in a decent sized city, about 500,000 people, where would I find dry malt and dextrose. Would a grocery store have that, that's microbrew supplies right?

When nocking up LC jars, how much solution should I use (a couple CCs)?

Alright that's all I got for questions. Thanks for the help, I'm really excited now. Peacefulness. Again thanks.


--------------------
Load Universe into cannon. Aim at brain. Fire.

~Nailing Bee to the Honey

Edited by StoneInFocus (01/07/06 01:15 PM)

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Re: Need an easy liquid tek that works [Re: Synaethesia]
    #5150881 -

Mustard, Mayo, or any plastic PP5 lid that will screw on a glass jar.

Tubing, auto parts small bore rubber fuel or vacuum line.
(I just happen to use medical grade catheter tubing)

Home brew beer supply store for light dry malt & dry dextrose.

2 ml per jar is fine. Shake sh*t out of syringe - first.


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Re: Need an easy liquid tek that works [Re: agar]
    #5151157 -

Alright good deal, eternaly greatful to you, as are my neuron synapses.


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Load Universe into cannon. Aim at brain. Fire.

~Nailing Bee to the Honey

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Re: Need an easy liquid tek that works [Re: agar]
    #19242478 -

Interesting,, though not so technical


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Laughter is the best medicine
Fuck the modern medical system!

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Re: Need an easy liquid tek that works [Re: MycofestDestiny]
    #19243924 -

Fwiw, there's better ways to go 'semi-bulk' w/out risking contamination with lc. Going from brf to lc seems silly. Like climbing a learning cliff!

Esp if you're blending 'fresh mushies' and inoculating grain with that.:facepalm:

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Re: Need an easy liquid tek that works [Re: OldHam]
    #19244067 -

8 year thread guys...

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Re: Need an easy liquid tek that works [Re: PhenoDreamers]
    #19246456 -

I know, but if I post on here, I can access it easily from my threads for reference. Still learning how to use the site efficiently


--------------------
Laughter is the best medicine
Fuck the modern medical system!

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Re: Need an easy liquid tek that works [Re: MycofestDestiny]
    #19246844 -

There's a reason some threads haven't had activity in a long time. :shrug:

They're dead and shouldn't be messed with.
Please let the dead rest peacefully.  :zombiedrool:

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Re: Need an easy liquid tek that works [Re: Dark76]
    #19248034 -

I toats agree.
We should just let this heinous thread die a natural death. Just let it die people.


To recap: Everyone just continue to ignore it and don't address the question (no one uses the search function anyways...that's why these threads arent just erased from the archives after 5-6 years as opposed to hosting rubbish for decades... Just let the dead stay dead for His sake. His = Gods.

And :blah:  :blah:  :blah: 

:blast:

Edited by OldHam (12/08/13 11:51 PM)

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Re: Need an easy liquid tek that works [Re: OldHam]
    #19248130 -

There are some old threads that still have lots of good info. This one is over 9 years old and I still look at it when trying to think of ways to change up my media cheap and easy :shrug: I like to read through the old threads to get a sense as to how far we have come and how far we have yet to go. That being said LC is something that has had its day for cubensis and this one needs to drift back into the recesses of the board.

OP if you want to save something for later reference just click the icon below your sig with the string tied around the finger. This will save it in your post reminders on your account page. If your like me and post a lot, bookmarking a thread that no one posts in anymore is not going to help you find it fast.

Finally OP, please do not attempt an LC until after you have done agar and learned something about clean inoculate and sterile technique. Otherwise we are just going to see a post from you in two months asking why you just grew a metric shitton of trich.

Now we can let this thread die.

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