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Offlinepacodestroys
late nightresearcher
Registered: 06/30/03
Posts: 35
Loc: colorado
Last seen: 13 years, 17 days
spore printing
    #1674604 - 06/30/03 07:09 PM (14 years, 3 months ago)

AFAOF has been seeing alot of people saying how difficult spore printing is, I have 2 cakes beginning to fruit and am planning on making a spore print and syringe to innoculate some more jars. I was simply going sterilize a pint jar by swabbing with rubbing alcohol and cut a mature cap off and set in the bottom of the jar (with a pin, blah blah), then close the lid (which has four holes poked in top like a PF jar). 24 hours later remove cap and let sit to make a syringe later by squirting sterile water into the jar from numerous syringes and then filling them up. Any problems with this method? contams? Easiest way to sterilize syringe water?


--------------------
He wear no shoeshine he got toe-jam football
He got monkey finger he shoot coca-cola
He say "I know you, you know me"
One thing I can tell you is you got to be free
Come together right now over me


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InvisibleLiL_KuSsH
Lost In The Trip

Registered: 05/07/03
Posts: 3,001
Deleted [Re: pacodestroys]
    #1674618 - 06/30/03 07:13 PM (14 years, 3 months ago)

Post deleted by administrator


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Offlinepacodestroys
late nightresearcher
Registered: 06/30/03
Posts: 35
Loc: colorado
Last seen: 13 years, 17 days
Re: spore printing [Re: LiL_KuSsH]
    #1674623 - 06/30/03 07:14 PM (14 years, 3 months ago)

yes, immediately, I have 40 jars ready to innoculate


--------------------
He wear no shoeshine he got toe-jam football
He got monkey finger he shoot coca-cola
He say "I know you, you know me"
One thing I can tell you is you got to be free
Come together right now over me


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Offlinepacodestroys
late nightresearcher
Registered: 06/30/03
Posts: 35
Loc: colorado
Last seen: 13 years, 17 days
Re: spore printing [Re: pacodestroys]
    #1674636 - 06/30/03 07:18 PM (14 years, 3 months ago)

Will this method work if I use the print immediately?


--------------------
He wear no shoeshine he got toe-jam football
He got monkey finger he shoot coca-cola
He say "I know you, you know me"
One thing I can tell you is you got to be free
Come together right now over me


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InvisibleLiL_KuSsH
Lost In The Trip

Registered: 05/07/03
Posts: 3,001
Deleted [Re: pacodestroys]
    #1674722 - 06/30/03 07:54 PM (14 years, 3 months ago)

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InvisibleRipple
Ripple
Male User Gallery

Folding@home Statistics
Registered: 05/16/02
Posts: 21,014
Loc: the timbers of Fennario
Trusted Cultivator
Re: spore printing [Re: pacodestroys]
    #1674774 - 06/30/03 08:15 PM (14 years, 3 months ago)

Your method will work if you are very careful : Take common sense steps like washing hands, spray Lysol and cover the 4 little holes just to be safe.


--------------------
The bus came by and I got on that's when it all began!



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InvisibleLiL_KuSsH
Lost In The Trip

Registered: 05/07/03
Posts: 3,001
Deleted [Re: Ripple]
    #1674785 - 06/30/03 08:19 PM (14 years, 3 months ago)

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OfflinemindXplorer
mind xplorer

Registered: 10/19/02
Posts: 85
Last seen: 9 years, 5 months
Re: spore printing [Re: LiL_KuSsH]
    #1674835 - 06/30/03 08:38 PM (14 years, 3 months ago)

This is the 200,000 post in Mushroom cultivation!


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InvisibleRipple
Ripple
Male User Gallery

Folding@home Statistics
Registered: 05/16/02
Posts: 21,014
Loc: the timbers of Fennario
Trusted Cultivator
Re: spore printing [Re: LiL_KuSsH]
    #1674851 - 06/30/03 08:43 PM (14 years, 3 months ago)

:tongue: 


--------------------
The bus came by and I got on that's when it all began!



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Offlinehyper_dermic
stranger withcandy

Registered: 06/26/02
Posts: 736
Loc: the land of excess
Last seen: 13 years, 1 month
Re: spore printing [Re: Ripple]
    #1674860 - 06/30/03 08:46 PM (14 years, 3 months ago)

actually if u cover the holes, the humidity from the rotting mushroom will most likely leave a pile of goo on the bottem of the jar rendering the print useless...

i would suggest covering it with some sort of breathable material, like a paper towel....

another way to stop the sucker from rotting is putting it in the fridge.. i havnt tested this out yet, but i plan to next flush....

im gonna take a ziplock dinner tray and cut a hole in the top and place some tyvek over it to act as a filter.
then place shroom + index card inside, and toss the whole fucker in the fridge for a day or 2....

hopefully this will solve the drying-out/rotting problems ive had in the past....

good luck to you, whichever method u choose to use...

[hyp]


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OfflineRaedon
enthusiast

Registered: 04/07/03
Posts: 271
Last seen: 11 years, 4 months
Re: spore printing [Re: hyper_dermic]
    #1674880 - 06/30/03 08:57 PM (14 years, 3 months ago)

The hardest part of spore printing, beyond sterile tek, is knowing when to cut the top off a shroom and print it. An easy way is to just watch the caps on the shrooms around the tallest one. The second the tops of the smaller shrooms starts to turn darker from spores falling, go for it.


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Offlinekindkesey
take it further
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Registered: 03/16/03
Posts: 2,789
Loc: on the bus!!!
Last seen: 3 years, 10 months
Re: spore printing [Re: hyper_dermic]
    #1674897 - 06/30/03 09:06 PM (14 years, 3 months ago)




Yes, it will work. If you use a 1/2 pint ball jar, most syringes wont reach the bottom of the jar to scrape the spores into your solution. So your holes will be useless. You can find 1/4 pint jars if you look hard, they work great for my hippie friend.


--------------------
Stay Kool, and enjoy the bus ride.....



"Intrepid Search For Innerspace"

DAVID JONES where are you?


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Invisibletekramrepus
Female User Gallery
Registered: 02/20/02
Posts: 2,229
Re: spore printing [Re: kindkesey]
    #1674971 - 06/30/03 09:39 PM (14 years, 3 months ago)

Ive been cultivating "Cultivating what? I dont know" since OCtober, and although Ive had MANY, MANY jars fall victim to contams....

Ive made over 15 syringes, and never ran across a problem with contams.

Its not hard, just be super sterile


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