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I got the PE to grow on agar, what now? (Updated with pics!)
    #12342736 -

But I need a little advise for favor. There is one small section with rizo growth, are the different sectors the spaces between the long rizo strands? If so how do I choose what sector to transfer to the next agar plate?


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Edited by Grizzdude (05/03/10 01:36 AM)

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Re: I got the PE to grow on agar, what now? [Re: Grizzdude]
    #12342821 -

Grab the rhizo stuff. Transfer some to new agar, some right to grain for spawning.


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You make it all possible. Doesn't it feel good?

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Re: I got the PE to grow on agar, what now? [Re: Doc_T]
    #12345136 -

Thanks doc!


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Re: I got the PE to grow on agar, what now? [Re: Grizzdude]
    #12497201 -

Ok so I transfered agar to agar last week and this is what I got for the PE, I took 3 ryzo sections from my PE dish and now they look pretty good. 2 of the dishes have this pink dot crap going on.





And here is an Oakridge strain that I am trying to clone. I also took 3 ryzo sections from the dish and this is what I got now.




How do things look to you guy's? How many more transfers do you think I need? Any advise, comments on the PE or Oakridge are welcomed


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Re: I got the PE to grow on agar, what now? [Re: Grizzdude]
    #12497219 -

looking good! :thumbup:  I may someday try agar.  Seems like a fun experiment.

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Re: I got the PE to grow on agar, what now? [Re: Josh84]
    #12499072 -

I'm kinda wondering if there is still sectoring on the PE? The top picture looks like it might have 5 or 6 sectors but I'm really not sure?


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Re: I got the PE to grow on agar, what now? [Re: Grizzdude]
    #12499316 -

Nice rizo ropes on the OR!

I'm a relative agar newb with only a few isolates under my belt, but the PE dishes look like they need have several sectors and could use another round of transfers.


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Re: I got the PE to grow on agar, what now? [Re: Grizzdude]
    #12499395 -

Most of the time you need to do like four or five transfers before you start seeing individual sectors.


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ephemeral anomalous

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