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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Where's the hole?
    #11161922 -

So I just inoculated my first batch. I'm growing Ecuador cubensis with rye casing method. When it came time to introduce the spores to their habitat I had problems finding my inoculation hole. I had a Tyvek filter, so the best I could do was look at the cap from underneath and try and guess where the hole was. When I first prepared the jars I tried using a marker to mark where the hole was, but when I screwed the lid on they became mismatched. How do you go about finding your hole? Any useful information would be greatly appreciated. Thanks

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Re: Where's the hole? [Re: Herbal Answer]
    #11161986 -

rtv your tyvek in place with a dab on both sides serving 2 purposes

a. self healing
b. mark your hole


--------------------
:shrug:

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Re: Where's the hole? [Re: nastos]
    #11162010 -

If your using RTV, why are you using Tyvek???  There would be no use for Tyvek if your plugging the hole up with RTV.  No hole to breathe through no need to Tyvek.....


--------------------
"I celebrate the Earth, my home, my mother, my grave, and as long as men are Man they must, if they would preserve the integrated being, do the same---[and preserve]--this rank casual hungry smelly sweaty lusting transitory body, my oozy pulpy liquid-bag-swollen body, bones, blood, hair glands, my bejeweled sex; I love and celebrate it all.  never to let men forget that they are animals as much as gods---that is one thing I shall say."

  Edward Abbey

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Re: Where's the hole? [Re: hawksapprentice]
    #11162014 -

Or do you not mean to plug the hole up???


--------------------
"I celebrate the Earth, my home, my mother, my grave, and as long as men are Man they must, if they would preserve the integrated being, do the same---[and preserve]--this rank casual hungry smelly sweaty lusting transitory body, my oozy pulpy liquid-bag-swollen body, bones, blood, hair glands, my bejeweled sex; I love and celebrate it all.  never to let men forget that they are animals as much as gods---that is one thing I shall say."

  Edward Abbey

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Re: Where's the hole? [Re: hawksapprentice]
    #11162016 -

sometimes there is an injection hole and a breather hole dude...  the breather hole need not be found as it will serve its purpose under the tyvek..


--------------------
:shrug:

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Re: Where's the hole? [Re: nastos]
    #11162024 -

wear latex gloves and feel for the hole with your hands. use some hand sanitizer if you are paranoid like me.

but why diddnt you just mark the hole after screwing the lid on?

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Re: Where's the hole? [Re: nastos]
    #11162026 -

i used to have the same problem.bent quite a few needles missing the hole and hitting the plastic lid through the self healing port.
simple solution.used a biro pen to mark the hole when making the lids.



the lids in the picture are very simple.
heat an awl red hot.
make two holes.
cover one hole with piece of old innertube glued in place with ordinary puncture repair glue.mark the hole!
push/pull some polifil through the other and your done.

good luck


--------------------
whether low pressure sucks or high pressure blows...
it's a bugger to cycle in.

even though I'm feeling good
Something tells me I'd better activate my prayer capsule

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Re: Where's the hole? [Re: Herbal Answer]
    #11162053 -

Herbal Answer said:
  I had problems finding my ... hole.


That's what she said!

:facepalm: hahaha, couldn't help myself. but no, seriously, in order to prevent damaging your needles, i make it real easy on myself and after I've assembled my jars, but before i load them into the PC, i use a soft lead pencil<easier to mark on the tyvek,any pencil should work> to gently mark where the hole is located. just like filling in the bubbles on the test sheets at school, i make the mark large enough to see through my glovebox. i don't use ink because it tends to bleed when PC'ed or swabbed with alcohol.
  and don't toss that needle that's bent up. you can fix it with some careful surgery, using a pair of needle nosed pliers, and some fine grit sandpaper. just straighten the tip out with your pliers, then lay the sandpaper on a hard surface and lightly drag the tip over the sand paper at the same angle the the tip is beveled.


--------------------
"-If we look at the path, we do not see the sky. We are earth people on a spiritual journey to the stars. Our quest, our earth walk, is to look within, to know who we are, to see that we are connected to all things, there is no separation, only in the mind-"
                                Native American-source unknown

  "Peace can only come as a natural consequence of Universal Enlightenment."
                                Nikola Tesla-1919

6T's WBS tek

Whaddya mean CASING?

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Re: Where's the hole? [Re: nonamenomad]
    #11169343 -

Damn, the solution is simple. I dunno why I didn't think about marking the hole AFTER screwing the lids on. I may have to have the doctor check me for some sort of retardation....Thanks to all that replied. I hadn't heard of RTV and will have to look into it. Thanks again.

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Re: Where's the hole? [Re: Herbal Answer]
    #11169365 -

problem solved


1/8th inch hole filled with silicone
1/4 inch hole covered with tyvek held on by micropore tape

you can see thru the silicone so you wont bend your needles
and you have a hole for GE as well

i do all my lids like this and never have any problems with them


  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.
Citric said:
Your signature is wrong on colonization temps!
GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT
ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef

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Re: Where's the hole? [Re: noobieshroomie]
    #11169410 -

I just make my holes right in the middle of the lid after experiencing troubles like yours.


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there
  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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