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Invisiblerobinhood2
merry man w/outthe tights!
Registered: 07/22/02
Posts: 110
rye grain problem
    #849368 - 08/28/02 03:24 PM (19 years, 5 months ago)

This is my friend's first time using rye grain. He's got quart jars, polyfill filter hole, rye was cooked in pressure cooker with 250ml grain (1 cup), and 200-210 ml of water. Innoculated with 2cc of spore water each. Stored at 79-86 degrees. The grains look cooked, minimal exploded grains, and some grains at the bottom have dried out a lil and stuck, despite vigorous shaking and mixing after cooking.

6 days after innoculation, my friend sees absolutely no growth. No mold, no rhyzomorphic growth, no cobwebs, nothing. The syringes had been stored in his fridge. What might explain this here? Bad syringe? Spores died in fridge? Is it bad to re-innoculate?

Robin


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OfflineChromeCrow
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Re: rye grain problem [Re: robinhood2]
    #849409 - 08/28/02 03:53 PM (19 years, 5 months ago)

how long did he wait after steralizing,to innoculate?


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InvisibleRoadkillM
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Re: rye grain problem [Re: robinhood2]
    #849417 - 08/28/02 03:58 PM (19 years, 5 months ago)

How long did you pc the Grain?
15 psi at 90 minutes?

Did you let the PC cool down on the stove without letting off the pressure in it?

How old were the spore syringes?

You don't want to put more spores in those jars.

You probably should have seen some growth already......wait a few more days.

good luck!


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Invisiblerobinhood2
merry man w/outthe tights!
Registered: 07/22/02
Posts: 110
Re: rye grain problem [Re: Roadkill]
    #849459 - 08/28/02 04:23 PM (19 years, 5 months ago)

OK just to answer the questions.....

Pressure cooked for an hour, like most teks said to...wasn't aware of 90min.

Cooled down in the cooker, no rushing

Jars cooled overnight before innoculatiing.

Syringes were a few months old....from the spring, stored in fridge.


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InvisibleHippie3
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Re: rye grain problem [Re: robinhood2]
    #850347 - 08/29/02 04:05 AM (19 years, 5 months ago)

where did you shoot the spores ?
should be along the side, so you can see growth,
not in the middle.


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Edited by Hippie3 (08/29/02 04:06 AM)


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OfflineAnnoA
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Re: rye grain problem [Re: Hippie3]
    #850360 - 08/29/02 04:18 AM (19 years, 5 months ago)

Hmm, it was rye, so he was supposed to shake it after inoculation, doesn?t matter where you inoculate....


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InvisibleHippie3
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Re: rye grain problem [Re: Anno]
    #850362 - 08/29/02 04:20 AM (19 years, 5 months ago)

i never shake after inoculation,
only after there's some visible growth do i shake it to speed colonization.
i find it works either way.
here's a text sample from a rye grain tek at my site-
In reply to:


The procedure for inoculating a jar is as follows:

1) Shake the syringe very well to agitate the spore solution within the cylinder. Check to make sure that any air bubbles are worked out of the cylinder.

2) Flame or sterilize the tip of the needle.

3) Remove the tape covering the hole in the lid and carefully insert the tip of the needle into the hole. ( steps 2 and 3 are done at the same time so the needle is still sterile when it reaches the inside of the jar.)

4) Aiming for the sides of the glass (directly above the grain), squeeze the spore solution into the jar while rotating the syringe needle around the inner jar in a circular fashion making sure to spread the solution out around the whole circumference of the jar. You will need to pay close attention not to go over 2-3 cc's per jar. You can position the syringe in your hand so that you are able to read the markings better. It may help to draw markings on the cylinder with a pen to help serve as a guide. Eventually, you should be able to just go by "feel" and come suprisingly close to the mark every time

5) pull the syringe out of the jar and recover with the tape as rapidly as possible.

6) wipe the syringe clean with an alcohol-saturated towel and set aside.

7) Mark the jar with a pen to indicate the necessary information for your reference.




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Edited by Hippie3 (08/29/02 04:28 AM)


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Offlinebabyshroom
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Registered: 09/09/01
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Re: rye grain problem [Re: Hippie3]
    #850376 - 08/29/02 04:51 AM (19 years, 5 months ago)

Ah....2 mycologists with vast amounts of experience and totally different opinions. I'm always torn as to whether I should shake or not. I do shake before innoculation, but it seems that I have more failure with jars that I shake again after innoculation. This is only the case with liquid innoculant. When I do gtg transfers, I shake with great results.
Shaking is shaking, right? I mean, I can't possibly be so stupid that I'm shaking them incorrectly, can I?


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OfflineAnnoA
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Re: rye grain problem [Re: Hippie3]
    #850389 - 08/29/02 05:18 AM (19 years, 5 months ago)

>i find it works either way.

This is true.


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InvisibleHippie3
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Re: rye grain problem [Re: Anno]
    #851547 - 08/29/02 05:36 PM (19 years, 5 months ago)

i did a batch 2day your way, anno.
but i'm guessing that way works better when more inoculant is used, so i put in 5 cc of spore solution per and shook hell out of it.
we'll see if that's better .


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Offlineindkebr
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Re: rye grain problem [Re: Hippie3]
    #853195 - 08/30/02 12:59 PM (19 years, 5 months ago)

"4) Aiming for the sides of the glass (directly above the grain), squeeze the spore solution into the jar while rotating the syringe needle around the inner jar in a circular fashion making sure to spread the solution out around the whole circumference of the jar. You will need to pay close attention not to go over 2-3 cc's per jar. You can position the syringe in your hand so that you are able to read the markings better. It may help to draw markings on the cylinder with a pen to help serve as a guide. Eventually, you should be able to just go by "feel" and come suprisingly close to the mark every time "

My question is for the statment: You will need to pay close attention not to go over 2-3 cc's per jar.

So you can't use a whole syringe and make the speed increase? I have pleny of syringes, and was planningo n using a full syringe in each jar. Is this not a good idea?


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Offlineindkebr
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Registered: 06/21/02
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Loc: Colorado
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Re: rye grain problem [Re: indkebr]
    #853612 - 08/30/02 06:10 PM (19 years, 5 months ago)

bump


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Offlinecaptiveball
just anotherbuddah
Registered: 07/29/02
Posts: 30
Last seen: 19 years, 3 months
Re: rye grain problem [Re: indkebr]
    #853694 - 08/30/02 06:50 PM (19 years, 5 months ago)

only used about 2-3 cc's and my quart is 85% done 10 days in

you need it faster that that?

slow down you move to fast
gotaa make the mornin last


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InvisibleHippie3
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Re: rye grain problem [Re: indkebr]
    #854331 - 08/31/02 04:00 AM (19 years, 5 months ago)

you can get by with 5 cc but more than that usually leads to problems with water pooling in the bottom


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Offlineindkebr
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Re: rye grain problem [Re: Hippie3]
    #854703 - 08/31/02 11:22 AM (19 years, 5 months ago)

ok thanks for the clarification.


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