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The procedure for inoculating a jar is as follows:

1) Shake the syringe very well to agitate the spore solution within the cylinder. Check to make sure that any air bubbles are worked out of the cylinder.

2) Flame or sterilize the tip of the needle.

3) Remove the tape covering the hole in the lid and carefully insert the tip of the needle into the hole. ( steps 2 and 3 are done at the same time so the needle is still sterile when it reaches the inside of the jar.)

4) Aiming for the sides of the glass (directly above the grain), squeeze the spore solution into the jar while rotating the syringe needle around the inner jar in a circular fashion making sure to spread the solution out around the whole circumference of the jar. You will need to pay close attention not to go over 2-3 cc's per jar. You can position the syringe in your hand so that you are able to read the markings better. It may help to draw markings on the cylinder with a pen to help serve as a guide. Eventually, you should be able to just go by "feel" and come suprisingly close to the mark every time

5) pull the syringe out of the jar and recover with the tape as rapidly as possible.

6) wipe the syringe clean with an alcohol-saturated towel and set aside.

7) Mark the jar with a pen to indicate the necessary information for your reference.

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