mushroomhunter10 said:

I would just poke 3-4 very small holes in your lids, cut some tyvek to shape, and go to town in your glove box when it's time to inoculate.

Really easy to do...

Can you post the link to 6T's Tek??

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Quote:

fastfred said:Silicone will however impair gas exchange.  There's really no point to it.  You don't want, nor do you need, a sealed system.  People have come up with way too many methods to solve problems that don't exist.

Injecting through the polyfill works just fine.  You don't even need the polyfill.  It only serves as a potential wick for contamination.  Polyfill is usually only used in the lab for test tubes, mainly because it's a little faster than tearing up all that tinfoil.

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libertaire said:

Better safe than sorry, that's what I say.  The advantage I could see to a self sealing system is that you can be a complete slob, or at least a naive noob, and still have a fairly good chance of success.  You do still have to be slightly sterile, but I do think it decreases chances for failure quite a bit.  Both techniques are completely contam proof.  Obviously there's no way that contams could make it through a silicone barrier, so long as the needle/spore solution is completely sterile, but polyfil, if packed densely enough, is impossible for contaminates to penetrate, more so than any sort of tape, or certainly more than nothing at all.

Also, both silicone and polyfil are easily purchased in most areas, so why not take advantage of some special precautions that will at least somewhat possibly increase your chances of success?  Sterility is hard, at least for some people.  Outsmarting contaminates is easy if you use your noggin.  I guess it's just two ways of reaching the same goal, not saying one is right or wrong.

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libertaire said:

Just sticking up for your techniques man.

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