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Re: Step by step, first time in a long TIME (with pics) [Re: fastfred]
    #11048072 -

fastfred said:
I used to use polyfill. But it's just not worth it.  It works as well or better to simply leave a hole in the top.  No need for it at all.


-FF



When the jars are colonizing you don't use a filter just open to the air?


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

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Re: Step by step, first time in a long TIME (with pics) [Re: scrotesmcgrotes]
    #11048085 -

I plan on making a glove box tonight or tomorrow.  I have a big plastic thing that down comforters come in and some rubber gloves.  I'm going to burn some holes in the side, fit the gloves in and the seal it up.  Spray the inside with lysol/wipe it down with rubbing alcohol.

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Re: Step by step, first time in a long TIME (with pics) [Re: scrotesmcgrotes]
    #11048089 -

scrotesmcgrotes said:
mushroomhunter10 said:
You don't have to do the 18 hour soak... not required. I've never had to and never will. Just more time to waste IMHO.




I've got time and as I'm in grad school, this is keeping me from studying, which is a good thing in my book.



Not a good thing. Study your dick off.

Anyways, NICE pressure cooker. I'm jealous. I can only fit two quart jars in mine, or 7 half-pints! :grin:


--------------------
Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!

Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES

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Re: Step by step, first time in a long TIME (with pics) [Re: scrotesmcgrotes]
    #11048092 -

I was thinking about the tyvek, but I'm comfortable with polyfil at the moment.  Perhaps next time, I will use tyvek.

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Re: Step by step, first time in a long TIME (with pics) [Re: scrotesmcgrotes]
    #11048103 -

Tyvek is so much easier... IMHO of course.

Yes and remember, no heat sources in the box!!


--------------------
Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!

Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES

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Re: Step by step, first time in a long TIME (with pics) [Re: mushroomhunter10]
    #11048115 -

mushroomhunter10 said:
Not a good thing. Study your dick off.

Anyways, NICE pressure cooker. I'm jealous. I can only fit two quart jars in mine, or 7 half-pints! :grin:



Don't worry, I have been.  This is the start of year two (just two more to go after this!) so I know what it takes.  It's good to have hobbies though, and as I quit drinking, I need a little something to occupy the time.  Mushrooms and I have always gotten along well, so I thought I'd take up the old hobby and see what comes of it.

On that note, I am going to go study (I need to memorize the new edition of the APA manual, study for candidacy exams, and get ready for my thesis proposal meeting on Tuesday).  Good times.

Thanks for the suggestions and help.  I'll throw some more pics up tomorrow when the glove box is done, jars are PCd and I'm getting ready to inoculate.

Enjoy your evenings, all.

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Re: Step by step, first time in a long TIME (with pics) [Re: truskool]
    #11048167 -

truskool said:
fastfred said:
I used to use polyfill. But it's just not worth it.  It works as well or better to simply leave a hole in the top.  No need for it at all.


-FF



When the jars are colonizing you don't use a filter just open to the air?



No, you still have a tinfoil lid.  I just finally figured out it was pointless after dong a few rounds.  In the lab we never use anything more than tinfoil caps on our flasks.  There's just no point, contams fall down any lid will prevent this.

See the thread:
http://www.shroomery.org/forums/showflat.php/Number/7857880

A lot of people just can't seem to wrap their mind around the fact that a lot of what people do is a complete waste of time.

Another tip BTW.  Rye works a LOT better than WBS.  It's a lot less contamination prone.  WBS contams easier than anything else I've tried.


-FF

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Re: Step by step, first time in a long TIME (with pics) [Re: scrotesmcgrotes]
    #11048172 -

Good Luck


--------------------
Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!

Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES

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Re: Step by step, first time in a long TIME (with pics) [Re: scrotesmcgrotes]
    #11048180 -

Oh.
You only need like 1/4 cc per jar (sometimes not even), don't just gush it in there. Shake those syringes well too.

Don't want to waste them. Put them in the fridge when not in use, the viability factor will remain higher for a longer period of time.

Peace


--------------------
Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!

Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES

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Re: Step by step, first time in a long TIME (with pics) [Re: mushroomhunter10]
    #11048210 -

Oh, good to know.  So I should be able to do all 7 quart jars with 1 syringe?  Any thought on the strains?  Treasure Coast, Hualta, Costa Rica and PES Hawaiian?

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Re: Step by step, first time in a long TIME (with pics) [Re: fastfred]
    #11048225 -

I have been having mad conatim problems and thought that it was due to my new place but at the same time could be the fact that I started using tyvek without the foil before tyvek I just used foil. FF you may have just convinced me to say screw the tyvek and go back to straight foil.  I never knew about the contaim difference of rye vs WBS I use WBS cause of the cost and access.  I may go back to using rye again.


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

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Re: Step by step, first time in a long TIME (with pics) [Re: scrotesmcgrotes]
    #11048236 -

Those Hawaiians are a nice strain, and ironically just a marketing ploy. Psilocybe cubensis doesn't grow in Hawaii!!

However, a lot of people here love them, huge caps and very good visuals. Some say a cube is a cube, I disagree.

Yes all 7 quarts with a syringe easily.

Shoot at two opposing sides of each jar, 1/4 cc on each side. :grin:

Then throw them bitches in the fridge. Don't forget to flame sterilize the needles and wipe with alcohol in between jars.

Good Luck


--------------------
Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!

Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES

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Re: Step by step, first time in a long TIME (with pics) [Re: mushroomhunter10]
    #11048246 -

This is good, but going LC that one syringe will produce forever until contaminated


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

Extras: Filter Print Post Top
Re: Step by step, first time in a long TIME (with pics) [Re: truskool]
    #11048260 -

True, he could make mycelium water and/or an lc with just 1/8 of a cc into a 1/2 pint.

Good idea truskool!


--------------------
Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!

Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES

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Re: Step by step, first time in a long TIME (with pics) [Re: mushroomhunter10]
    #11048277 -

And the amount of time you gain by doing LC after the LC has grown vs MS growth you cut your future grows down by days at the minimum to weeks.


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

Extras: Filter Print Post Top
Re: Step by step, first time in a long TIME (with pics) [Re: truskool]
    #11048289 -

:thumbup:


--------------------
Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!

Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES

Extras: Filter Print Post Top
Re: Step by step, first time in a long TIME (with pics) [Re: mushroomhunter10]
    #11048290 -

I read about the Hawaiian scam in the strains thread.  Marketing is the scourge of modern life, but c'est la vie.

Making LC is on my to-do list.  First I want to make sure I can grow successfully, then I still step up my game.  This is just a test run.  Also, I pretty much just want these to give to friends/self.  I have no interest in selling these and the potential shit you can get yourself into with that.  Just personal use and close trusted friends.  While more is always better, I don't know if I need to go the whole LC route.  A few casings here and there should last me a long time.

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Re: Step by step, first time in a long TIME (with pics) [Re: scrotesmcgrotes]
    #11048308 -

scrotesmcgrotes said:
I read about the Hawaiian scam in the strains thread.  Marketing is the scourge of modern life, but c'est la vie.

Making LC is on my to-do list.  First I want to make sure I can grow successfully, then I still step up my game.  This is just a test run.  Also, I pretty much just want these to give to friends/self.  I have no interest in selling these and the potential shit you can get yourself into with that.  Just personal use and close trusted friends.  While more is always better, I don't know if I need to go the whole LC route.  A few casings here and there should last me a long time.



This is true yet an LC in the fridge will last awhile save time and when you want to do the odd jar just slam it with an LC and never buy a syringe again.


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

Extras: Filter Print Post Top
Re: Step by step, first time in a long TIME (with pics) [Re: scrotesmcgrotes]
    #11048329 -

Yeah but an LC in the fridge can probably last you a few years. :grin:

Plus LC begins colonizing immediately, no germination required.

Just use
96% water
4% honey

PC for 20 minutes, good to go! Oh and no holes in the lids of the LC's!


--------------------
Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!

Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES

Extras: Filter Print Post Top
Re: Step by step, first time in a long TIME (with pics) [Re: mushroomhunter10]
    #11048401 -

Well, then when this cycle is over I will make some LC for myself and offer up any extra syringes to friends who might want to try their hand at mycology.

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Shop: North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds


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