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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Rye Grain, Vermiculite

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Invisiblevildechayea
shodan

Registered: 10/03/02
Posts: 107
black specks in colonizing jars
    #982268 - 10/22/02 10:22 AM (15 years, 1 month ago)

In my last set of 1/2 pints (brf + rye flour) I chucked a jar that was showing black specks. Now my latest set of jars (brf + millet) is showing small black specks, some slightly rod-shaped, within the white beginning areas of colonization only.

Most likely to me would be clumped spores, since it only appears in colonizing areas...wouldn't a contam attack the uncolonized substrate first?

All opinions appreciated. Peace!

v


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Offlinesluglee
i am dino.

Registered: 07/26/02
Posts: 272
Loc: Jurassic Park
Last seen: 10 years, 3 months
Re: black specks in colonizing jars [Re: vildechayea]
    #982348 - 10/22/02 11:07 AM (15 years, 1 month ago)

sorry to tell you that, your jar has fallen to contamination it is now dead...


--------------------
Slug Lee
still shroomin' ...


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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: black specks in colonizing jars [Re: vildechayea]
    #982500 - 10/22/02 12:21 PM (15 years, 1 month ago)

It does not sound good but don't do anything rash. Separate the black speck jars from the others and wait. If it is a contam you'll know in a few days. Good luck.


--------------------


Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


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Offlinebluethumbs
em pleh
Registered: 03/01/02
Posts: 25
Last seen: 15 years, 1 day
Re: black specks in colonizing jars [Re: dog]
    #982529 - 10/22/02 12:32 PM (15 years, 1 month ago)

are you sure that its not some peice of vermiculite...?


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OfflineSkikid16
fungus fan

Registered: 06/27/02
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Re: black specks in colonizing jars [Re: bluethumbs]
    #982703 - 10/22/02 01:49 PM (15 years, 1 month ago)

He didn't say if he was using verm or not all he said was brf+rye and brf+millet. Do what dog said, seperate and wait. BTW, its interesting that you used brf in conjunction with millet and rye, both are usually used by themselves, not saying that you did anything wrong, I just haven't heard all that much about combining them. Out of curiosity, how did you sterilize these jars? Good luck.


--------------------
Re-Defeat Bush in '04


Edited by Skikid16 (10/22/02 01:52 PM)


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Invisiblevildechayea
shodan

Registered: 10/03/02
Posts: 107
Re: black specks in colonizing jars [Re: Skikid16]
    #982837 - 10/22/02 02:46 PM (15 years, 1 month ago)

Thanks all for input.

The jars are PF formula, verm + brf, but then I've also tried adulterating the brf portion by say 25-40% with other grains just to see how they affect growth rate. The batch with some rye flour mixed in seems to be colonizing slightly quicker than usual. The millet I hope will provide more sustained nutrition over time.

I sterilize by boiling the jars in a large stock pot, washcloth on the bottom, and water about half way up the jar sides. Previously I heated them for 1-1/2 hours, this time about 1-1/4 hours.

A few minutes ago, I went back and checked the remaining syringes and they're super clumpy in there...I think that's my answer. When I did the jars (they are all displaying the specks), despite shaking, the inoculant was rather clumped, and the areas now starting to colonize provide a white background for spotting the clumped spores. I think that the fact the specks are only appearing in colonized areas is the key. And they look more like black pepper, rather than fuzzy or blotchy spots.

Guess we'll see. Thanks again for all your help.

v


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OfflineSkikid16
fungus fan

Registered: 06/27/02
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Re: black specks in colonizing jars [Re: vildechayea]
    #982889 - 10/22/02 03:05 PM (15 years, 1 month ago)

If you sterilized your grain by boiling, then it may very likely be contams, grain is harder to sterilize than BRF, and almost always needs to be sterilized using a PC. Well, hopefully, somehow, maybe you will get lucky, but next time you may want to try PC'ing your grain. Good Luck


--------------------
Re-Defeat Bush in '04


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Invisiblevildechayea
shodan

Registered: 10/03/02
Posts: 107
Re: black specks in colonizing jars [Re: Skikid16]
    #987244 - 10/23/02 06:48 PM (15 years, 1 month ago)

Thanks for all your help.

The mycelium are ripping thru those jars right now like Sherman thru Atlanta.

Methinks that millet kernels being so small and dry might get sterilized enough through boiling, but I defer to others more experienced.

Peace & goos luck!

v


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OfflineRichard Gere


Registered: 02/01/12
Posts: 13
Last seen: 1 year, 11 months
Re: black specks in colonizing jars [Re: vildechayea]
    #15766300 - 02/05/12 11:41 PM (5 years, 9 months ago)

I am having the same problem. I inoculated pint jars and it is taking forever to colonize. Just read that 1/2 pint is the way to go for cakes. I also forgot to shake syringe. I am curious! One has a black spot that has been there for weeks but the spot has barely grown. My most active jar by the way. If it does not grow is it still a contam or bacteria?

RG


Edited by Richard Gere (11/11/14 05:00 PM)


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OfflineCircleofFifths
Can I has a mushy?


Registered: 09/21/11
Posts: 336
Loc: Right behind YOU!!!
Last seen: 4 years, 6 months
Re: black specks in colonizing jars [Re: Richard Gere]
    #15767217 - 02/06/12 07:12 AM (5 years, 9 months ago)

IME, the black "rod" shaped specs are just clumps of dried spores. I just used a syringe that I made myself that had the "rods" in it. Also I have noticed that if you use a lighter or candle to flame sterilize, sometimes the soot comes off in the jars and that also makes black spots.

EDIT: I meant to say the if you use a lighter or candle to flame sterilize sometimes black soot comes off in the jars.


Edited by CircleofFifths (02/06/12 08:26 PM)


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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Rye Grain, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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