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Invisiblezeta
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Registered: 05/25/02
Posts: 3,972
Dung sterilization / pasteurization
    #956330 - 10/13/02 04:52 AM (18 years, 11 months ago)

I have dung that's been sitting outside for a few weeks and I need to do something with it..
I have previously pasteurised by microwaving on high for 10 minutes and it worked fine, but this just seems too easy compared to keeping it at 170 for 90 minutes (I have no thermometer so doing that isn't an option). My other option is pressure cooking it, I know some people do this successfully. Could I get some thoughts on the matter.
Thanks


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InvisibleZen Peddler
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Registered: 06/18/01
Posts: 6,379
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Re: Dung sterilization / pasteurization [Re: zeta]
    #956335 - 10/13/02 04:54 AM (18 years, 11 months ago)

With cubies either way but if your growing pans then id always suggest that you sterilise


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OfflineItsAllGood
London Shroomer

Registered: 10/09/02
Posts: 175
Loc: London UK
Last seen: 18 years, 9 months
Re: Dung sterilization / pasteurization [Re: Zen Peddler]
    #956396 - 10/13/02 05:50 AM (18 years, 11 months ago)

Una suggests sterilisation of Dung/straw for pans. However other teks suggest past. coz of the high risk of contam of sterilised bulk substrate. I would go with Past. for Pans and use the old pillow and simmer trick unless you have a flow hood.


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Invisiblezeta
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Registered: 05/25/02
Posts: 3,972
Re: Dung sterilization / pasteurization [Re: ItsAllGood]
    #956398 - 10/13/02 05:54 AM (18 years, 11 months ago)

My lobster is growing cubies.
Why do people bother with the pillowcase in a pot for 90 minutes if 10 minutes in the microwave works just as well?


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OfflineItsAllGood
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Registered: 10/09/02
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Re: Dung sterilization / pasteurization [Re: zeta]
    #956402 - 10/13/02 05:57 AM (18 years, 11 months ago)

i love the smell of simmering shit !!!


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Invisibleralphster44
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Re: Dung sterilization / pasteurization [Re: zeta]
    #956411 - 10/13/02 06:19 AM (18 years, 11 months ago)

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InvisibleCptnMaxMushroom
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Registered: 08/26/02
Posts: 138
Re: Dung sterilization / pasteurization [Re: zeta]
    #956617 - 10/13/02 10:54 AM (18 years, 11 months ago)

Proper pasteurization takes many hours, it's not done right by putting it in a microwave for 10 minutes on high. If anything, thats sterilization.

However, you should still have some success.



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Invisibledurban_poison
myco contractor
Registered: 09/19/01
Posts: 2,415
Re: Dung sterilization / pasteurization [Re: zeta]
    #956634 - 10/13/02 11:17 AM (18 years, 11 months ago)

can somebody please show me evidence that sterilized manure contams easier the pasturized. I have only noticed the oppisite is true, my fire ants only sterilize because past. has never been successful. Maybe past works for cubs becasue of there speedy growth rate but my fire ants always fail with past.


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OfflineAnnoA
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Re: Dung sterilization / pasteurization [Re: durban_poison]
    #956783 - 10/13/02 01:37 PM (18 years, 11 months ago)

>can somebody please show me evidence that sterilized manure contams easier the pasturized.

When you pasteurize, you can inoculate the substrate more or less in the open, since the pasteurized substrate has some resistance to contamination.

If you STERILISE the substrate, you HAVE TO inoculate and colonize it in sterile conditions, ie inoculate in front of a flow hood and colonize in a spawn bag for instance.

If I personaly want to do small amounts of substrate, then I sterilize, if I have bigger amounts, I pasteurize.

For me, the sterilized dung conaminates less than the pasteurized. The downside is that the sterilization and inoculation of big amounts is technicaly more requiring.


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InvisibleZen Peddler
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Re: Dung sterilization / pasteurization [Re: Anno]
    #959132 - 10/14/02 07:02 AM (18 years, 11 months ago)

Sure, but as we all know, pans dont compete well with contams and i cant be fucked waiting for hours for my shit to boil up. pc for an hour seal it in a ziplock and spawn it - never had a problem


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InvisibleTheLoopIs
Registered: 05/29/13
Posts: 330
Re: Dung sterilization / pasteurization [Re: zeta]
    #18407568 - 06/12/13 11:42 AM (8 years, 3 months ago)

what if Brown rice flour is also in the mix? Since it contams much easier I heard?
I think i still want to pasteurize, is that a good idea?


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