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Anonymous

Re: Using agar grown mycelium that has overtaken mold?
    #92260 - 01/31/00 07:51 AM (22 years, 7 months ago)

anyone at all? anyone can answer this, i'm not picky...
Oleander

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I'm only two people away from having a menage a trois!



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Offlineloathe
enthusiast
Registered: 01/26/00
Posts: 204
Last seen: 20 years, 3 months
Re: Using agar grown mycelium that has overtaken mold?
    #92262 - 01/31/00 08:59 PM (22 years, 7 months ago)

You should clone the mycelium from that side of the dish, then colonize and fruit, and feed the mushies to someone you're not too fond of. If they die, then I wouldn't suggest using that strain again ..

That's all I can think of. (c:

------------------
"Woe to you my Princess, when I come, I will kiss you quite red and feed you till you are plump. See who is the stronger, a gentle little girl who doesn't eat enough or a big wild man who has cocaine in his body."
-- Sigmund Freud, On Coca



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Anonymous

Re: Using agar grown mycelium that has overtaken mold?
    #92263 - 02/02/00 09:15 AM (22 years, 7 months ago)

I would try to clone it.
Its not like having a cake that is contaminated and than overtaken by mycelium (where the contam toxins have prolly been released to the cake), this time your only using to clone. If you do clone I would clone for at least 2-3 times before moving to grain for fruiting. This way you can be sure there are no toxins left from the first plate contams. But it could be that the contam merged with the mycelium and they grow sied by side, only you cant see it.

If you see any contams in the cloned plate, I would use that mycelium. If not, you decide if you take the chance

Later,
tweedy.



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OfflineWorkmanV
Psilocybe Microscopist
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Registered: 03/01/01
Posts: 3,566
Loc: Washington, USA
Last seen: 3 years, 9 months
Trusted Cultivator
Re: Using agar grown mycelium that has overtaken mold?
    #92264 - 02/05/00 12:00 AM (22 years, 7 months ago)

Mycelium over growing contamination on agar plates is common. Agar formulations are designed to favor mushroom mycelium. If a mold or bacteria fails to thrive on the agar, it can be overgrown by the mycelium. The mycelium that over grew the mold is no different than the mycelium just before it contacted the mold. So if you are going to clone it, don't use the mold covered area. Loose mold spores could recontaminate the new plate.

I am afraid your mycelium is not a super contamination eater but it does have good vigor and should grow quickly and fruit abundantly.



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Anonymous

Re: Using agar grown mycelium that has overtaken mold?
    #92265 - 02/06/00 09:58 PM (22 years, 7 months ago)

I think you should clone it


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Anonymous

Re: Using agar grown mycelium that has overtaken mold?
    #92266 - 02/10/00 01:23 AM (22 years, 7 months ago)

I was reading in my fat biology book about this kinda thing. They had pennecillin, which kills bacteria, and they exposed a large amount of bacteriums to this pennecillin. Now like 1/1000 of the bacteria survived, just a tiny speck. They took this speck and grew it on a seperate petri dish. Then they introduced it to pennecillin again. The pennecillin had no affect on it. This is natural selection.

Try to gather your mycelium which overgrows molds and bacteria, and it may have a naturally selected immunity to them. It is just an experiment.

------------------
Flog and squee
up the tree
see the army flee, flee, flee.



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Offlinelares
member
Registered: 12/14/98
Posts: 129
Last seen: 16 years, 10 months
Re: Using agar grown mycelium that has overtaken mold?
    #92267 - 02/10/00 10:33 PM (22 years, 7 months ago)

Actually, no, this is artificial selection. Very important difference.


--------------------
"The universe does a math equation that never even ever really is anywhere in it, and if it spouts out creation, we're on the tip of its tongue, and it asks us where we stand." -- Modest Mouse


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InvisibleSclorch
Clyster

Folding@home Statistics
Registered: 07/13/99
Posts: 4,805
Loc: On the Brink of Madness
Re: Using agar grown mycelium that has overtaken mold?
    #92268 - 02/13/00 03:37 AM (22 years, 7 months ago)

Actually, I would prefer to use the mycelium that overtook the mold. If it is strong enough to do it once, it can do it again. Right now I'm conducting several experiments, with different species of hallucinogenic shrooms, to see which species is better at fighting contamination. Surprise surprise, right now it looks like the wild strain (Florida) of psilocybe cubensis is the toughest. But the shrooms are only in the first stage of testing (colonization), the fruiting stage experiments should be interesting. I'll keep you updated.


--------------------
Note: In desperate need of a cure...


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