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InvisibleGGreatOne234
Stranger
Registered: 12/24/99
Posts: 8,946
Alternatives to Agar Plates?
    #867439 - 09/06/02 03:34 PM (19 years, 1 month ago)

Hey Shroomers,

I hope this fits well in Advanced Cultivation
-I also looked through the FAQ's & did a search for this so,

Here's the deal,
My agar plates are history.
-But we still have plenty of P.D.A. & yeast left.

Anyone ever try using 1/2pint or 1pint jars instead of the damn petri's?
-Or are there any suitable alternatives besides the petri's?
--Like from the grocerty store or whatnot.

Could anyone solve my dilema without having to order new petri plates.?

Thanks,
Keep shroomin,
GG :smirk: 


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Offlinepaddo
Oh shit i realydid it this time
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Registered: 03/16/02
Posts: 591
Loc: Netherlands
Last seen: 3 years, 15 days
Re: Alternatives to Agar Plates? [Re: GGreatOne234]
    #867466 - 09/06/02 03:42 PM (19 years, 1 month ago)

Quote:

Anyone ever try using 1/2pint or 1pint jars instead of the damn petri's?





Yes it works.

It has been done And with succes! :smile:

Seen a tek somewhere on the net but couldnt find it anymore.

Paddo 


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InvisibleGGreatOne234
Stranger
Registered: 12/24/99
Posts: 8,946
Re: Alternatives to Agar Plates? [Re: paddo]
    #867552 - 09/06/02 04:34 PM (19 years, 1 month ago)

You have seen a tech on the net but cannot remember..
-Anyone else remember???  :smile: :smile: :smile:

-Oh goodie, i think i should be able to figure this out then..

But i wonder which one would work better,
The 1/2pint or the 1pints..

..also, i wonder if i could pour agar into each jar and then steralize for the 20 minutes
--This way would bypass the pouring of the hot liquid..??

Any other input on this subject would be nice,

The lab technicians would like to do this project this weekend.

thanks,

Keep shroomin,
GG 


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Offlinebluepoo
journeyman
Registered: 03/27/02
Posts: 83
Loc: europe
Last seen: 19 years, 1 month
Re: Alternatives to Agar Plates? [Re: GGreatOne234]
    #867837 - 09/06/02 08:41 PM (19 years, 1 month ago)

iv tride soap boxs i got at a pound shop then clean with alchole not great
try clean the plates with alchole after a wash or two with dilute bleach the trick is to get a stirle place to let them dry
jars work fine though but 20 plates cost me E3.50 so i dont bother and always get 95% contam free from stirle print and some wat less from wild strains
washing and drying takes too long and are only about 60-70% cotam free


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-----------------------------------------------------------
the above is what the litel leprecans told me to say and should be regarded as such
-----------------------------------------------------------


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OfflineShaw
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Registered: 06/28/01
Posts: 2,220
Loc: midwest, usa
Last seen: 10 months, 1 day
Re: Alternatives to Agar Plates? [Re: GGreatOne234]
    #867848 - 09/06/02 08:46 PM (19 years, 1 month ago)

yah, it's pretty common.
there are a few teks about this. Shouldn't be hard to find


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InvisibleGGreatOne234
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Registered: 12/24/99
Posts: 8,946
Re: Alternatives to Agar Plates? [Re: bluepoo]
    #868198 - 09/07/02 05:20 AM (19 years, 1 month ago)

Cool, Thanks everyone,

I will spend more time today looking for more info,

Thanks again,
Keep shroomin,
GG


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Offlineaural
Hola Amigos!
Registered: 05/04/00
Posts: 511
Loc: The Desert In The Spring
Last seen: 17 years, 6 months
Re: Alternatives to Agar Plates? [Re: GGreatOne234]
    #868437 - 09/07/02 08:35 AM (19 years, 1 month ago)

1/2 pints are easier to work with,but deep enough that you can sterilize the agar "in place" without any hassle.

That about all the tek you need!


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InvisibleGGreatOne234
Stranger
Registered: 12/24/99
Posts: 8,946
Re: Alternatives to Agar Plates? [Re: aural]
    #868465 - 09/07/02 08:56 AM (19 years, 1 month ago)

Excellent,

By saying "in place" - i assume that you mean you can pour the unsterilzed agar mixture into the 1/2pints Before sterilizeing them in the pressure cooker??


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OfflineShaw
hick

Registered: 06/28/01
Posts: 2,220
Loc: midwest, usa
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Re: Alternatives to Agar Plates? [Re: GGreatOne234]
    #868530 - 09/07/02 09:41 AM (19 years, 1 month ago)

yah, just use them like agar plates. Why do you need a special tek?


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OfflineVisigoth
enthusiast

Registered: 11/28/01
Posts: 481
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Re: Alternatives to Agar Plates? [Re: Shaw]
    #868698 - 09/07/02 11:45 AM (19 years, 1 month ago)

Hell yeah, this idea sounds great!!! Being able to seal the jar by tightening down the lid would be much easier and effective in keeping out contaminants, as opposed to using plastic wrap or parafin right?? And what would be better, lifting the lid to inncoculate or poking a small hole in the lid and covering w. tape afterwards?? Am I correct in my assumptions?? Thanks everyone!!!

Vis


--------------------
"15 minutes in freezing conditions?!?! You'd become a popsicle before you made that!!!"


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: Alternatives to Agar Plates? [Re: Visigoth]
    #868714 - 09/07/02 12:01 PM (19 years, 1 month ago)

If your going to do "clones" from fresh mushroom material, rizo material off anything, or wedges from another plate? Your going to have to open 1/2 pints, to  do so.

But, no big deal -- IF -- you have a clean way to do it (clean room, glove box or flow hood).

Hell, a foaf started agar use with baby food jars & that worked fine.

SixTango  :tongue:

 


--------------------
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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Offlineaural
Hola Amigos!
Registered: 05/04/00
Posts: 511
Loc: The Desert In The Spring
Last seen: 17 years, 6 months
Re: Alternatives to Agar Plates? [Re: GGreatOne234]
    #868887 - 09/07/02 02:26 PM (19 years, 1 month ago)

In reply to:

By saying "in place" - i assume that you mean you can pour the unsterilzed agar mixture into the 1/2pints Before sterilizeing them in the pressure cooker??





You got it! One less chance for contamination.Though if you are doing cultures as oppsed to spores then I highly recommend peroxide agar.


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InvisibleGabbaDj
BTH
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Registered: 04/08/01
Posts: 19,589
Loc: By The Lake
Re: Alternatives to Agar Plates? [Re: GGreatOne234]
    #869038 - 09/07/02 04:23 PM (19 years, 1 month ago)

If you can make Agar then you should really try pint jars...

Make as usual and PC.. Then twirl the jars around and spread the agar up the sides of the jar as far as possible...
An uncontaminated jar will grow mycellium up and around it all over, the stuff looks like its taking over...
Throw an uncontaminated penny in the jar and fill with water. Shake vigorusly then extract liquid with syringe and youve now got syringes that work better than and spore syringe.

I will never use plates again..


--------------------
GabbaDj

FAMM.ORG          C8.com                    http://www.beatsopjefiets.com/   


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InvisibleGGreatOne234
Stranger
Registered: 12/24/99
Posts: 8,946
Re: Alternatives to Agar Plates? [Re: GabbaDj]
    #869255 - 09/07/02 06:22 PM (19 years, 1 month ago)

"Then twirl the jars around and spread the agar up the sides of the jar as far as possible..."

-Well i did 5 Pint jars today, but i did not twirl the jars around like you suggested. Seems like a great fu*kin idea though dude!!!

I will do the "twirl" next time.  :laugh: :laugh: :laugh: lol

Keep shroomin,
GG


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OfflineJOptionPane
I AM THE WARCHIEF
Registered: 08/20/02
Posts: 120
Last seen: 17 years, 11 months
Re: Alternatives to Agar Plates? *DELETED* [Re: GGreatOne234]
    #869347 - 09/07/02 07:20 PM (19 years, 1 month ago)

Post deleted by JOptionPane


--------------------
I do not currently cultivate any mushrooms. Nor have I cultivated any mushrooms in the past. Nor will I cultivate any mushrooms in the near or distant future.




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Offlineaural
Hola Amigos!
Registered: 05/04/00
Posts: 511
Loc: The Desert In The Spring
Last seen: 17 years, 6 months
Re: Alternatives to Agar Plates? [Re: JOptionPane]
    #869948 - 09/08/02 11:14 AM (19 years, 1 month ago)

One reason 1/2 pts are better than baby food jars,IMO,is that it is recommended to use wedges from close to the center of the agar before mycelium gets to the sides of the jar.With baby food jars,that doesn't give you much latitude.
Also,canning jars seal easier and cleaner than commercial jars...


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InvisibleGGreatOne234
Stranger
Registered: 12/24/99
Posts: 8,946
Re: Alternatives to Agar Plates? [Re: GGreatOne234]
    #872145 - 09/09/02 11:26 AM (19 years, 1 month ago)

Well the lab tech's did the 5 one pint jars
And i am rather happy with this.

So i instructed them to crank out the old HEPA,

And innoculate the following;
3 with Psilocybe weilii
&
2 with Panaeolus castaneifolius

The technicians will keep me posted
&
I will do the same for you all.

Thanks again,


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InvisibleGGreatOne234
Stranger
Registered: 12/24/99
Posts: 8,946
Re: Alternatives to Agar Plates? [Re: GGreatOne234]
    #874226 - 09/10/02 10:11 AM (19 years, 1 month ago)

Well folks,

I think that this may be working.

One of the Psilocybe weilii Pint jars is taking off!!!!
:laugh: :tongue: :shocked: :grin: :blush: :smile: 


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OfflineVisigoth
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Registered: 11/28/01
Posts: 481
Last seen: 8 years, 8 months
Re: Alternatives to Agar Plates? [Re: GGreatOne234]
    #875100 - 09/10/02 05:03 PM (19 years, 1 month ago)

Yeah, I did some jars last night of agar. They dried and look good. Today I scraped some spores onto two of em. So we'll see how it goes. I''ll post an update later on!!

Vis


--------------------
"15 minutes in freezing conditions?!?! You'd become a popsicle before you made that!!!"


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OfflineVisigoth
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Registered: 11/28/01
Posts: 481
Last seen: 8 years, 8 months
Re: Alternatives to Agar Plates? [Re: Visigoth]
    #876986 - 09/11/02 08:21 AM (19 years, 1 month ago)

Two questions:

1. Is it ok to store already poured plates of agar in the fridge until ready to use?

2. Is it ok to turn the plates upside down so that condensation will not drip and land on the agar?

Thanks!!!!

Vis


--------------------
"15 minutes in freezing conditions?!?! You'd become a popsicle before you made that!!!"


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InvisibleGGreatOne234
Stranger
Registered: 12/24/99
Posts: 8,946
Re: Alternatives to Agar Plates? [Re: Visigoth]
    #877123 - 09/11/02 09:14 AM (19 years, 1 month ago)

Let me take a stab at this one guys,

Visigoth,

1. Is it ok to store already poured plates of agar in the fridge until ready to use?
-From what i have learned, the fridge might be the most contaminated zone of your house. I would not store petri's in the fridge unless they were all wrapped, sealed and put into a contaminant-free container..

2. Is it ok to turn the plates upside down so that condensation will not drip and land on the agar?
-No need for this. The condensation does no harm..

?? Correct/Expand on any of my answers if needed..

Keep shroomin,
GG


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Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   PhytoExtractum Kratom Powder for Sale   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Bridgetown Botanicals CBD Edibles   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Original Sensible Seeds Bulk Cannabis Seeds, High THC Strains


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