Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

Magic Mushrooms Zamnesia
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Advanced Mycology
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   Sporeworks.EU Spores for European Microscopy   Bridgetown Botanicals CBD Concentrates   PhytoExtractum Kratom Powder for Sale

Jump to first unread post Pages: 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Alternatives to Agar Plates?
    #867439 -

Hey Shroomers,

I hope this fits well in Advanced Cultivation
-I also looked through the FAQ's & did a search for this so,

Here's the deal,
My agar plates are history.
-But we still have plenty of P.D.A. & yeast left.

Anyone ever try using 1/2pint or 1pint jars instead of the damn petri's?
-Or are there any suitable alternatives besides the petri's?
--Like from the grocerty store or whatnot.

Could anyone solve my dilema without having to order new petri plates.?

Thanks,
Keep shroomin,
GG :smirk: 

Extras: Filter Print Post Top
Re: Alternatives to Agar Plates? [Re: GGreatOne234]
    #867466 -

Quote:
Anyone ever try using 1/2pint or 1pint jars instead of the damn petri's?




Yes it works.

It has been done And with succes! :smile:

Seen a tek somewhere on the net but couldnt find it anymore.

Paddo 

Extras: Filter Print Post Top
Re: Alternatives to Agar Plates? [Re: paddo]
    #867552 -

You have seen a tech on the net but cannot remember..
-Anyone else remember???  :smile: :smile: :smile:

-Oh goodie, i think i should be able to figure this out then..

But i wonder which one would work better,
The 1/2pint or the 1pints..

..also, i wonder if i could pour agar into each jar and then steralize for the 20 minutes
--This way would bypass the pouring of the hot liquid..??

Any other input on this subject would be nice,

The lab technicians would like to do this project this weekend.

thanks,

Keep shroomin,
GG 

Extras: Filter Print Post Top
Re: Alternatives to Agar Plates? [Re: GGreatOne234]
    #867837 -

iv tride soap boxs i got at a pound shop then clean with alchole not great
try clean the plates with alchole after a wash or two with dilute bleach the trick is to get a stirle place to let them dry
jars work fine though but 20 plates cost me E3.50 so i dont bother and always get 95% contam free from stirle print and some wat less from wild strains
washing and drying takes too long and are only about 60-70% cotam free


--------------------
-----------------------------------------------------------
the above is what the litel leprecans told me to say and should be regarded as such
-----------------------------------------------------------

Extras: Filter Print Post Top
Re: Alternatives to Agar Plates? [Re: GGreatOne234]
    #867848 -

yah, it's pretty common.
there are a few teks about this. Shouldn't be hard to find


--------------------

Extras: Filter Print Post Top
Re: Alternatives to Agar Plates? [Re: bluepoo]
    #868198 -

Cool, Thanks everyone,

I will spend more time today looking for more info,

Thanks again,
Keep shroomin,
GG

Extras: Filter Print Post Top
Re: Alternatives to Agar Plates? [Re: GGreatOne234]
    #868437 -

1/2 pints are easier to work with,but deep enough that you can sterilize the agar "in place" without any hassle.

That about all the tek you need!

Extras: Filter Print Post Top
Re: Alternatives to Agar Plates? [Re: aural]
    #868465 -

Excellent,

By saying "in place" - i assume that you mean you can pour the unsterilzed agar mixture into the 1/2pints Before sterilizeing them in the pressure cooker??

Extras: Filter Print Post Top
Re: Alternatives to Agar Plates? [Re: GGreatOne234]
    #868530 -

yah, just use them like agar plates. Why do you need a special tek?


--------------------

Extras: Filter Print Post Top
Re: Alternatives to Agar Plates? [Re: Shaw]
    #868698 -

Hell yeah, this idea sounds great!!! Being able to seal the jar by tightening down the lid would be much easier and effective in keeping out contaminants, as opposed to using plastic wrap or parafin right?? And what would be better, lifting the lid to inncoculate or poking a small hole in the lid and covering w. tape afterwards?? Am I correct in my assumptions?? Thanks everyone!!!

Vis


--------------------
"15 minutes in freezing conditions?!?! You'd become a popsicle before you made that!!!"

Extras: Filter Print Post Top
Re: Alternatives to Agar Plates? [Re: Visigoth]
    #868714 -

If your going to do "clones" from fresh mushroom material, rizo material off anything, or wedges from another plate? Your going to have to open 1/2 pints, to  do so.

But, no big deal -- IF -- you have a clean way to do it (clean room, glove box or flow hood).

Hell, a foaf started agar use with baby food jars & that worked fine.

SixTango  :tongue:

 


--------------------
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~

Extras: Filter Print Post Top
Re: Alternatives to Agar Plates? [Re: GGreatOne234]
    #868887 -

In reply to:
By saying "in place" - i assume that you mean you can pour the unsterilzed agar mixture into the 1/2pints Before sterilizeing them in the pressure cooker??




You got it! One less chance for contamination.Though if you are doing cultures as oppsed to spores then I highly recommend peroxide agar.

Extras: Filter Print Post Top
Re: Alternatives to Agar Plates? [Re: GGreatOne234]
    #869038 -

If you can make Agar then you should really try pint jars...

Make as usual and PC.. Then twirl the jars around and spread the agar up the sides of the jar as far as possible...
An uncontaminated jar will grow mycellium up and around it all over, the stuff looks like its taking over...
Throw an uncontaminated penny in the jar and fill with water. Shake vigorusly then extract liquid with syringe and youve now got syringes that work better than and spore syringe.

I will never use plates again..


--------------------
GabbaDj

FAMM.ORG             

Extras: Filter Print Post Top
Re: Alternatives to Agar Plates? [Re: GabbaDj]
    #869255 -

"Then twirl the jars around and spread the agar up the sides of the jar as far as possible..."

-Well i did 5 Pint jars today, but i did not twirl the jars around like you suggested. Seems like a great fu*kin idea though dude!!!

I will do the "twirl" next time.  :laugh: :laugh: :laugh: lol

Keep shroomin,
GG

Extras: Filter Print Post Top
Re: Alternatives to Agar Plates? *DELETED* [Re: GGreatOne234]
    #869347 -

Post deleted by JOptionPane


--------------------
I do not currently cultivate any mushrooms. Nor have I cultivated any mushrooms in the past. Nor will I cultivate any mushrooms in the near or distant future.



Extras: Filter Print Post Top
Re: Alternatives to Agar Plates? [Re: JOptionPane]
    #869948 -

One reason 1/2 pts are better than baby food jars,IMO,is that it is recommended to use wedges from close to the center of the agar before mycelium gets to the sides of the jar.With baby food jars,that doesn't give you much latitude.
Also,canning jars seal easier and cleaner than commercial jars...

Extras: Filter Print Post Top
Re: Alternatives to Agar Plates? [Re: GGreatOne234]
    #872145 -

Well the lab tech's did the 5 one pint jars
And i am rather happy with this.

So i instructed them to crank out the old HEPA,

And innoculate the following;
3 with Psilocybe weilii
&
2 with Panaeolus castaneifolius

The technicians will keep me posted
&
I will do the same for you all.

Thanks again,

Extras: Filter Print Post Top
Re: Alternatives to Agar Plates? [Re: GGreatOne234]
    #874226 -

Well folks,

I think that this may be working.

One of the Psilocybe weilii Pint jars is taking off!!!!
:laugh: :tongue: :shocked: :grin: :blush: :smile: 

Extras: Filter Print Post Top
Re: Alternatives to Agar Plates? [Re: GGreatOne234]
    #875100 -

Yeah, I did some jars last night of agar. They dried and look good. Today I scraped some spores onto two of em. So we'll see how it goes. I''ll post an update later on!!

Vis


--------------------
"15 minutes in freezing conditions?!?! You'd become a popsicle before you made that!!!"

Extras: Filter Print Post Top
Re: Alternatives to Agar Plates? [Re: Visigoth]
    #876986 -

Two questions:

1. Is it ok to store already poured plates of agar in the fridge until ready to use?

2. Is it ok to turn the plates upside down so that condensation will not drip and land on the agar?

Thanks!!!!

Vis


--------------------
"15 minutes in freezing conditions?!?! You'd become a popsicle before you made that!!!"

Extras: Filter Print Post Top
Re: Alternatives to Agar Plates? [Re: Visigoth]
    #877123 -

Let me take a stab at this one guys,

Visigoth,

1. Is it ok to store already poured plates of agar in the fridge until ready to use?
-From what i have learned, the fridge might be the most contaminated zone of your house. I would not store petri's in the fridge unless they were all wrapped, sealed and put into a contaminant-free container..

2. Is it ok to turn the plates upside down so that condensation will not drip and land on the agar?
-No need for this. The condensation does no harm..

?? Correct/Expand on any of my answers if needed..

Keep shroomin,
GG

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2  [ show all ]

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   Sporeworks.EU Spores for European Microscopy   Bridgetown Botanicals CBD Concentrates   PhytoExtractum Kratom Powder for Sale

Mushrooms, Mycology and Psychedelics > Advanced Mycology
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Culture storage and agar plate alternative. SalviaEngland 1,941 2 01/18/03 05:48 PM
by ISH
* Best pre-made agar plates for Cultivation no-tone 3,888 9 01/30/03 10:31 AM
by no-tone
* Agar plate info? Joshua 3,427 6 11/02/01 04:36 PM
by dimitri211
* Effect of gravity on agar plate? Mycolomaniac 1,566 3 04/07/22 07:32 PM
by primordialkraken
* Agar plates dog 1,375 3 04/05/05 09:45 AM
by Arp
* 1st and 2nd Flush from the Agar Plate! jelsert 1,023 1 12/07/02 01:28 PM
by Skikid16
* agar plate to liquid culture, how do u do it?? Visigoth 7,562 12 07/31/02 03:33 AM
by Suntzu
* wrapping agar plates with saran wrap
( 1 2 all )		 Green_Velvet 9,462 27 09/05/05 09:22 AM
by CrashManFlash

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta
5,808 topic views. 0 members, 0 guests and 1 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.006 seconds on 14 queries.