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Anonymous

Peroxidated agar and species sensitivity?
    #2607984 - 04/27/04 08:41 PM (12 years, 10 months ago)

Anyone have any problems with mexicana and peroxidated agar. I have clones of sclerotia that are taking forever to grow across the agar.

Concentration is 3 ml 3% per 500 ml media. The sclerotia recovered in 24 hours and was growing good mycelium at 48 hours, but it has taken over a week for it to even start to grow across the agar surface, and it has only grown about 1mm from the sclerotia. The growth seems very thick as well.

Is this typical of mexicana on peroxide, or does cubensis also have such a long lag time, and result in very slow growth across the media?

I am not liking the peroxide very much. Also experienced some piggy backing of a green mold. It grow from one small sclerotia, not visibly contaminated, onto the media. And another plate did contaminate with a browninsh green mold at the edge, even with the peroxide. Maybe the peroxide was not thouroughlydistributed, and some plates have more concentration then others?

Anyone with peroxide experience please check in.

It is growing just really slow.


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InvisibleJoshua
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Re: Peroxidated agar and species sensitivity? [Re: ]
    #2608211 - 04/27/04 09:27 PM (12 years, 10 months ago)

http://www.shroomery.org/forums/showflat...ev=#Post1315268

I haven't dealt with mexicana too much so can't tell you specifics.

I definately believe there is species sensitivity on peroxidated agar. I am sure concentration also has a lot to do with it.

If you have mold germinating on the peroxidated surface, I would ? your preparation. I have had peroxidated plates last without contamination until they dried up.

It is important to add the peroxide at a low temp as to not degrade it. What method did you use to prepare the peroxide addition to the agar?

Joshua


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Anonymous

Re: Peroxidated agar and species sensitivity? [Re: Joshua]
    #2608519 - 04/27/04 10:29 PM (12 years, 10 months ago)

Thanks for the link. I guess I should have done a search first.

Only two plates out of thirty contaminated and one was mold introduced with the explant, as mycelium not spores. The one freak plate that had perimeter contamination, who knows. My control plate left open for more then a second, is still clean.


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InvisibleJoshua
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Registered: 10/27/98
Posts: 5,389
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Re: Peroxidated agar and species sensitivity? [Re: ]
    #2609103 - 04/28/04 12:10 AM (12 years, 10 months ago)

I recently found out how sensitive a search can be, I'm less likely to give anyone crap...not that I ever did though.

Peace,
Joshua


--------------------
The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"


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