Home | Community | Message Board


Crestline Sales - MycoPath
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Seeds Store Buy Cannabis Seeds, Buy CBD   Amazon Agar, Autoclave, Petri Dish, Pressure Cooker

Jump to first unread post. Pages: 1
OfflinePeace_Patrol
Rambling hippie
Registered: 04/22/02
Posts: 350
Loc: Electric Neverending
Last seen: 13 years, 8 months
Need help with agar
    #746914 - 07/15/02 01:39 AM (15 years, 9 months ago)

Hey everyone
I am currently in the process of experimenting with the cultivation of Ps. Subaeruginosa from spores. Take note that subaeruginosa is a wood lover and essentially the same as cyanescens / azurescens as far as growing goes.
I was told a good way of starting a culture is by smearing spores on agar. So I cruised down to the pharmacy, asked for some agar, they gave me a weird look and eventually figured out that the didn't have agar, and to try 'one of the labs' so I went to a lab thing (blood testin place) and they gave me some weird agar in petri dishes. In one half of the dish is blood agar, and the other half says 'cled' whatever the hell that means. Either way, it appears neither of these support mycelium growth. Today a friend of mine gave me some agar powder because I'd asked if he knew where to get some. This agar is labelled as 'nutrient agar' and these are the ingredients:
Peptane :5
Yeast extract :2
Meat extract :1
Salt :5
Agar :15

And it says to use 28g/L of this powder per litre of water, sterilise in an autoclave for 15min at 121 degrees C.

So will this agar grow mycelium? If not, is there anything I can add to it so it will?
I don't know what the hell that 'meat extract' thing was. Could it be that my friend accidentally read it as meat instead of malt? (If so - what a meathead!) I'll double check tommorow.

Also, I don't have an autoclave, or a pressure cooker, can I still make the agar? Using an oven or something? I feel like a dumbass but I have never used agar for shrooms before so its kind of confusing.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,123
Loc: my room
Last seen: 1 month, 8 days
Re: Need help with agar [Re: Peace_Patrol]
    #747012 - 07/15/02 04:09 AM (15 years, 9 months ago)

>So will this agar grow mycelium?
No. It lacks sugar.

> If not, is there anything I can add to it so it will?
Yes, likely. Glucose.
Same amount as there is agar in it.

>Also, I don't have an autoclave, or a pressure cooker, can I still make the agar?
Look here:
http://shroomery.org/faq/faq.php?display=faq&nr=126&catnr=12&prog=1&lang=en&onlynewfaq=3

Also read http://www.mykoweb.com/articles/cultivation.html .


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinezeronio
Stranger
Male

Registered: 10/16/01
Posts: 2,349
Loc: Slovenia
Last seen: 1 year, 7 months
Re: Need help with agar [Re: Anno]
    #747096 - 07/15/02 05:43 AM (15 years, 9 months ago)

I think salt is not good for mushrooms.


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: Original Seeds Store Buy Cannabis Seeds, Buy CBD   Amazon Agar, Autoclave, Petri Dish, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* question about agar paddo 1,365 10 09/02/02 06:41 AM
by paddo
* Is it necessary to dry agar plates? shymanta 10,969 19 07/07/15 10:59 PM
by Demonsmoke
* Agar Strain Isolation - Is it worth it?
( 1 2 all )
Blue Helix 6,417 20 02/23/05 03:07 AM
by Blue Helix
* Some proven agar nutrient media recipes Una 5,667 11 10/03/04 03:15 PM
by YSr
* Interesting agar recipe (post corrected) MonkeyPod 2,572 10 04/18/05 01:18 PM
by MonkeyPod
* Agar help homebrew 615 2 01/08/02 02:11 AM
by homebrew
* Pectin 284_27 1,968 17 07/08/04 06:38 PM
by the man
* Black agar
( 1 2 all )
zeronio 6,578 23 01/19/08 12:32 AM
by RogerRabbit

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, bodhisatta
824 topic views. 1 members, 4 guests and 2 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Myco Supply
Please support our sponsors.

Copyright 1997-2018 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.006 seconds on 16 queries.