Home | Community | Message Board


High Mountain Compost
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Seeds Store Buy CBD, Cannabis Seeds, Compare CBD   Amazon Agar, Parafilm, Petri Dish

Jump to first unread post. Pages: 1
OfflineTriGuy
Skeptic

Registered: 08/28/99
Posts: 105
Loc: Georgia
Last seen: 14 years, 11 months
Re: Need Advice, storage of agar plates
    #91156 - 12/18/99 03:04 PM (17 years, 8 months ago)

Good hint: store them upsidedown to keep condensation off the medium.

Hawk: we need talk email me: triguy@bellsouth.net



Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleRyche HawkV
A Messenger
Male User Gallery

Registered: 03/01/01
Posts: 2,090
Re: Need Advice, storage of agar plates
    #91158 - 12/18/99 03:29 PM (17 years, 8 months ago)

.


--------------------
-Peace-

High Quality MUSHROOM SPORES and CULTURES  for microscopy at www.muShrooms.com


muShrooms.com is the new web site of
www.thehawkseye.com


Edited by Ryche Hawk (06/05/17 07:32 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Need Advice, storage of agar plates
    #91159 - 12/18/99 07:43 PM (17 years, 8 months ago)

ryche,
I usually store extra's in a gallon zip-lok bag.I dont have parafilm,but the bags seem to work.first I spray the bag with lysol,a few squirt's,then load in the petris and just before closing,spray a fw more time's with lysol..works very well..
As far as condensation,when I am in the glove-box and innoculating petri's I just give the lid a good shake,and off go the droplets.
later,fish

------------------



Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,123
Loc: my room
Last seen: 1 month, 22 days
Re: Need Advice, storage of agar plates
    #91160 - 12/19/99 07:25 AM (17 years, 8 months ago)

For sealing petri dishes I do the following:
I cut a 1 inch piece of a Saran Wrap role off and use this to wrap arroud the petri dish just like with Parafilm. You can strech the wrap a little bit and you will get a perfect tight seal, which is porous enough to alove a gas exchange though.
As for storing fresh plates: If they are not sterile, contamination will show up if you store them at room temeprature, so you can sort the bad ones out. If you store them in a fridge, you might end up transfering mycelium wedges to allready contaminated plates, without knowing it!

------------------
It?s easy to do without silver and gold
or even pleasures of love
but its hard to do without mushrooms.

Marcus Valerius Martialis
Roman poet



Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: Need Advice, storage of agar plates
    #91161 - 12/19/99 09:20 AM (17 years, 8 months ago)

Ryche, I use parafilm on my dishes and slants when in cold storage and place the them in a couple of large zip lock bags. When I need the plates, I remove the parafilm to inoculate and keep it off. I will lose a dish or two sometimes but not as much as w/the film. I have found over the years that agar needs to breathe.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleaaron
member

Registered: 10/18/99
Posts: 89
Re: Need Advice, storage of agar plates
    #91162 - 12/19/99 11:32 PM (17 years, 7 months ago)

Thats some good advice.
Place into individual ziplock bags then into gallon ziplock bags then into trash bags with twist ties,set on shelf and don't worry about it, its a lot faster than sealing them.
Reducing Condensation:
glass plates= sterilize so they are hot to the touch when you pour your agar.
try lowering your agar to 15 grams to 1000ml.
it won't set as fast,(so it can cool off longer) which will help on your condensation.

[This message has been edited by aaron (edited December 20, 1999).]



Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleRyche HawkV
A Messenger
Male User Gallery

Registered: 03/01/01
Posts: 2,090
Re: Need Advice, storage of agar plates
    #91163 - 12/20/99 01:58 AM (17 years, 7 months ago)

.


--------------------
-Peace-

High Quality MUSHROOM SPORES and CULTURES  for microscopy at www.muShrooms.com


muShrooms.com is the new web site of
www.thehawkseye.com


Edited by Ryche Hawk (06/05/17 07:37 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: Original Seeds Store Buy CBD, Cannabis Seeds, Compare CBD   Amazon Agar, Parafilm, Petri Dish

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* Culture storage and agar plate alternative. SalviaEngland 1,161 2 01/18/03 07:48 PM
by ISH
* Agar plate info? Joshua 2,013 6 11/02/01 06:36 PM
by dimitri211
* Agar plated some dried matter - Updates coming soon, got something growing...
( 1 2 3 all )
potatonet 6,452 50 04/05/09 11:18 PM
by potatonet
* Best pre-made agar plates for Cultivation no-tone 2,345 9 01/30/03 12:31 PM
by no-tone
* using old dehydated colonised mex-a agar plates ohmatic 1,522 8 09/23/07 01:01 PM
by Andrew47
* Is it necessary to dry agar plates? shymanta 9,730 19 07/07/15 10:59 PM
by Demonsmoke
* Agar plates & ciprofloxacin/penicillin the spiral 2,333 8 09/07/06 03:32 PM
by curenado
* Effect of gravity on agar plate? Mycolomaniac 852 2 03/30/04 03:27 AM
by Mycolomaniac

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, bodhisatta
2,201 topic views. 0 members, 6 guests and 1 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Gaiana.nl
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.031 seconds spending 0.007 seconds on 18 queries.