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Amazon Shop for: Agar, Laminar Flow Hood, Parafilm, Petri Dish

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OfflineTriGuy
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Registered: 08/28/99
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Re: Need Advice, storage of agar plates
    #91156 - 12/18/99 03:04 PM (17 years, 1 month ago)

Good hint: store them upsidedown to keep condensation off the medium.

Hawk: we need talk email me: triguy@bellsouth.net



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InvisibleRyche HawkV
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Re: Need Advice, storage of agar plates
    #91158 - 12/18/99 03:29 PM (17 years, 1 month ago)

Condensation is a problem. What I've been doing lately is letting the agar cool more.. although it becomes a little thicker, and doesnt quite poor as far.. I'm actually only loosing about 3 or 4 petri dishes of agar worth I figure. The ones that do still get condensation on them, I pull the lids off and wipe them dry with a pre-sterilized (in alcohol then dried) lint free cloth, KimWipes. I've noticed a lot less contams doing this. I'm also doing this in front of a flow hood though.

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High Quality MUSHROOM SPORES and CULTURES  for microscopy at www.muShrooms.com


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Anonymous

Re: Need Advice, storage of agar plates
    #91159 - 12/18/99 07:43 PM (17 years, 1 month ago)

ryche,
I usually store extra's in a gallon zip-lok bag.I dont have parafilm,but the bags seem to work.first I spray the bag with lysol,a few squirt's,then load in the petris and just before closing,spray a fw more time's with lysol..works very well..
As far as condensation,when I am in the glove-box and innoculating petri's I just give the lid a good shake,and off go the droplets.
later,fish

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OfflineAnnoA
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Re: Need Advice, storage of agar plates
    #91160 - 12/19/99 07:25 AM (17 years, 1 month ago)

For sealing petri dishes I do the following:
I cut a 1 inch piece of a Saran Wrap role off and use this to wrap arroud the petri dish just like with Parafilm. You can strech the wrap a little bit and you will get a perfect tight seal, which is porous enough to alove a gas exchange though.
As for storing fresh plates: If they are not sterile, contamination will show up if you store them at room temeprature, so you can sort the bad ones out. If you store them in a fridge, you might end up transfering mycelium wedges to allready contaminated plates, without knowing it!

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but its hard to do without mushrooms.

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Anonymous

Re: Need Advice, storage of agar plates
    #91161 - 12/19/99 09:20 AM (17 years, 1 month ago)

Ryche, I use parafilm on my dishes and slants when in cold storage and place the them in a couple of large zip lock bags. When I need the plates, I remove the parafilm to inoculate and keep it off. I will lose a dish or two sometimes but not as much as w/the film. I have found over the years that agar needs to breathe.


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Invisibleaaron
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Registered: 10/18/99
Posts: 89
Re: Need Advice, storage of agar plates
    #91162 - 12/19/99 11:32 PM (17 years, 1 month ago)

Thats some good advice.
Place into individual ziplock bags then into gallon ziplock bags then into trash bags with twist ties,set on shelf and don't worry about it, its a lot faster than sealing them.
Reducing Condensation:
glass plates= sterilize so they are hot to the touch when you pour your agar.
try lowering your agar to 15 grams to 1000ml.
it won't set as fast,(so it can cool off longer) which will help on your condensation.

[This message has been edited by aaron (edited December 20, 1999).]



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InvisibleRyche HawkV
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Re: Need Advice, storage of agar plates
    #91163 - 12/20/99 01:58 AM (17 years, 1 month ago)

Thanks for some input.
What I found was this.
All my agar plates I made a week or so again, then inoculated, then sealed with parafilm, then put in a large spawn bag and sealed were fine and growing mycelium. I had left a few in there, without any spores or cultures on them, no parafilm, all of them had mold on them. And some pretty wicked molds. Like 5 different kinds I had never seen before. They were actually pretty cool to sit and look at for a while, but in the end,, they got the alcohol bath.

Now for something interesting. When I made the agar plates, I made some with a tablespoon of zoo doo and an extra 25ml of water. The growth from the panaolus species on these plates was triple the size what it was on just plain agar. Anyone that has ever grown panaeolus on agar knows how slow this grows, but the addition of zoo doo really speeded it up.

I also noticed the p. alpina had a very spongy looking mycelium, and the p. mexican was very fast.. yet much more cottony then cubensis mycelium. Very pretty though.

Later in the week I'm ging to make some mycleium syringes and inoculate some bags of dung and zoo doo, and some finch seed as well.

-peace-



--------------------
-Peace-

High Quality MUSHROOM SPORES and CULTURES  for microscopy at www.muShrooms.com


muShrooms.com is the new web site of
www.thehawkseye.com
Sacred Mushroom Spores since the late 90's.


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Amazon Shop for: Agar, Laminar Flow Hood, Parafilm, Petri Dish

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