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Invisibledog
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Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Agar plates
    #4013325 - 04/04/05 06:20 PM (16 years, 6 months ago)

I've been working with agar plates with varying rates of success, and am wondering if the depth I'm pouring the agar has anything to do with it. Have you found an optimum depth to pour agar? Do different species thrive on different depths?

Thanks for your input.


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Offlinehyphae
born to grow
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Registered: 12/13/02
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Re: Agar plates [Re: dog]
    #4013706 - 04/04/05 07:57 PM (16 years, 6 months ago)

Thinner levels tend to dryout faster. I myself pour plates at half the depth or as close as possible. I also only use plates for isolating and I only use slants for storing stock cultures so I suppose I could certainly get by with thinner levels.


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OfflineRogerRabbitM
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Registered: 03/26/03
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Re: Agar plates [Re: hyphae]
    #4016381 - 04/05/05 10:55 AM (16 years, 6 months ago)

I pour about 1/4 full. Most species I grow out will fully colonize the petri dish within two weeks or so, and the lesser volume of agar will help to reduce condensation on the lid.

Hyphae, try something next time you put away cultures. Pour a couple of petri dishes very shallow. Perhaps a 1/8" layer. Allow the mycelium to colonize 2/3 of the dish. Be sure to use parafilm. Place in the refrigerator and forget it for a year or so. When you pull it out, the agar and mycelium will be totally, bone dry. Take a few samples and move them to fresh agar. Within a few days, they'll be growing again. I recently took out a three year old azurescens dish that was as dry as it could possibly be. It's growing great again, while the spores from the original print it came from were no longer viable. If I can confirm this with a few more trials, I'll be throwing away all my test tubes, as they're such a pain in the ass to prepare and clean out. I would have not thought dry mycelium could recover this well/fast.
RR


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InvisibleArp
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Registered: 04/20/98
Posts: 2,191
Loc: in a van by the river
Re: Agar plates [Re: RogerRabbit]
    #4016526 - 04/05/05 11:45 AM (16 years, 6 months ago)

Any1 else who experience fluffy growth with a thin layer?
I think I do :smile:


Edited by Arp (04/05/05 03:48 PM)


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