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Enema Bag Jones

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Best pre-made agar plates for Cultivation
    #1260610 - 01/30/03 05:48 AM (14 years, 2 months ago)

My friend may have access to many types of agar plates. I was wondering if anyone could tell me which of the following agar mixtures would be suitable in nutrients and pH for cultivation
TSA- Tryptic Soy Agar- 1 blue stripe
Nutrient- 2 blue stripes
SDA- Sabourands Dextrose agar - 1 green
Bacto- 2 green
MYM- 1 black
Marine- 2 black
TJA- Tomato Juice Agar - glucose - 1 red
TJA- sucrose- 2 red
MacKonkey's - 1 blue/ 1 red
MacKonkey's + Kanomycin - 1 blue/ 2 red
TSA +MgSO4 +(AMP) - 3 blue.

He also has access to Tryptic Soy Broth - a liquid medium, he thought it might be suitable for mycelial inncoulation.

Thanks a lot


--------------------
Man thinks. God laughs. - Jewish Proverb


Edited by no-tone (04/14/03 11:11 PM)


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Offlinedebianlinux
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Re: Best agar mixtures for Psilocybe [Re: no-tone]
    #1261156 - 01/30/03 09:27 AM (14 years, 2 months ago)

call me a dummy but what are the number/color combos? a cursory glance and the only recognizable possibility (to me) would be SDA.


Edited by debianlinux (01/30/03 09:27 AM)


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Enema Bag Jones

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Re: Best agar mixtures for Cultivation [Re: debianlinux]
    #1261237 - 01/30/03 09:54 AM (14 years, 2 months ago)

The stripes are to distinguish between plates. The stripe is on the plate itself, not the agar. Sorry about the confusion.


--------------------
Man thinks. God laughs. - Jewish Proverb


Edited by no-tone (04/14/03 11:15 PM)


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Enema Bag Jones

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Re: Best agar mixtures for Mushrooms [Re: debianlinux]
    #1261243 - 01/30/03 09:56 AM (14 years, 2 months ago)

Oh yes, the number preceeding the color is the amount of stripes. Example: 2 red = 2 red stripes side by side.


--------------------
Man thinks. God laughs. - Jewish Proverb


Edited by no-tone (04/29/03 12:27 PM)


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Re: Best pre-made agar plates for Psilocybe [Re: no-tone]
    #1261309 - 01/30/03 10:15 AM (14 years, 2 months ago)

Sabourauds is your best best bet amongst those listed.

MacKonkey and marine are probably the least likely to help you out.

I have had success using TSA, but it was absolutely inferior in performance to PDA or Sabourauds. [BTW, PDA outperforms Sabourauds, at least in several species I have tried including cubensis].

The one that I don't quite understand is 'Bacto'. Is that just the powdered agar? I have some 'Bacto' agar, the company has since been bought up. If it is the case, then this 'plain agar' can be combined with potato water, dextrose, and yeast extract [or yeast] to make fantastic fungal plates.
I don't know if that is the case, as the title of your post is 'premade agar plates'.

The problem with using TSB [the broth] is that it will grow just about ANYTHING. For a home cultivator, honey is actually a better option because it is somewhat selective. TSB will contaminate extremely easy at home [it's formulated for bacteria, sort of designed to contaminate]. If you are in a lab and can perform inoculations in a hood or such, it will work. There are better liquid formulae described in TMC/GGMM specific to our fungal friends.


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OfflineJackal
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Re: Best pre-made agar plates for Psilocybe [Re: no-tone]
    #1261315 - 01/30/03 10:16 AM (14 years, 2 months ago)

Why don't you do one of each in the name of research :wink:


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Enema Bag Jones

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Re: Best pre-made agar plates for Mushrooms [Re: no-tone]
    #1261714 - 01/30/03 12:09 PM (14 years, 2 months ago)

There is also a plate called 'spirit blue' the lab. it is only agar and contains no nutrients. Would it be worth boiling this down and adding nutrients?


--------------------
Man thinks. God laughs. - Jewish Proverb


Edited by no-tone (04/29/03 12:27 PM)


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Enema Bag Jones

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Re: Best pre-made agar plates for Mushrooms [Re: Jackal]
    #1261728 - 01/30/03 12:14 PM (14 years, 2 months ago)

I might be interested in that, but right now my mind is on getting some done to the finish.

I have a quite possibly contaminated spore print here that I made about a year back. If i do a streak for isolated colonies, do you think it would work out?

Or should i just order another syringe?


--------------------
Man thinks. God laughs. - Jewish Proverb


Edited by no-tone (04/29/03 12:26 PM)


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InvisibleShmoppy McGillicuddy
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Re: Best pre-made agar plates for Psilocybe [Re: no-tone]
    #1261738 - 01/30/03 12:17 PM (14 years, 2 months ago)

Try it! No point in buying some spores if you have some already. The worst that'll happen is the loss of a plate.

But, be sure to transfer a chunk to another plate as fast as possible, so if there are any contam's present, they don't have as much of a chance.


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Enema Bag Jones

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Re: Best pre-made agar plates for Mushrooms [Re: Suntzu]
    #1261764 - 01/30/03 12:31 PM (14 years, 2 months ago)

Now that I think about it, the probable reason why SDA plates dont work as well as PDA is because their pH ~4. Although SDA is a plate suited to fungi, it is a substrate that will inhibit most bacterial growth while remaining a suitable substrate for filamentous fungi (mold).


--------------------
Man thinks. God laughs. - Jewish Proverb


Edited by no-tone (04/29/03 12:25 PM)


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