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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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Isolation necessary for clones grown on agar?
    #903171 -

I was wondering if it would be benificial to transfer rhizormatic sections of clones that are grown on agar. One would think that if it is an isolate all the mycelium would grow identical. My eyes could be playing tricks on me, but it seems that there are both cottony and strandy sectors. Am I crazy, as all the mycelium from a clone is the same, go ahead and cut that sucker up and transfer to some grains, or should I isolate what could possibly be rhizormatic sectors? Thanks for any advice.

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Re: Isolation necessary for clones grown on agar? [Re: Green_Velvet]
    #903195 -

To be honest, I've never had problems transferring the cottony sectors from an isolate. They will grow out properly.


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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five

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Re: Isolation necessary for clones grown on agar? [Re: mickey_rourke]
    #903217 -

In reply to:
I've never had problems transferring the cottony sectors from an isolate.



I thought that if the myc was sectoring it was not yet isolated. Were these isolates taken from multispore or a clone. Thanks.

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Re: Isolation necessary for clones grown on agar? [Re: Green_Velvet]
    #903219 -

Both, and I have seen what looks very similar to sectoring with isolates.


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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five

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Re: Isolation necessary for clones grown on agar? [Re: mickey_rourke]
    #903222 -

me too


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hmmm........

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Re: Isolation necessary for clones grown on agar? [Re: mickey_rourke]
    #903312 -

In reply to:
They will grow out properly.



So do you think it would be a waste of time to tranfer out the strandy sectors to new dishes?

Edited by Green_Velvet (09/23/02 11:33 PM)

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Re: Isolation necessary for clones grown on agar? [Re: Green_Velvet]
    #906021 -

So does anyone know the anwser to my original question? Anno perhaps?

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Re: Isolation necessary for clones grown on agar? [Re: Green_Velvet]
    #906167 -

It was already answered... lol


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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five

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Re: Isolation necessary for clones grown on agar? [Re: Green_Velvet]
    #909621 -

I don't know this answer for sure, I'm pretty much making some eduacated guesses but I've read the mushroom cultivator and it explains that there are certain monokaryon that are compatible with others and some that are not which will produce what they called illegitimate matings. I'm thinking that the cottony sectors might have something to do with this, and so I think that it would still be best to isolate the rhizomorphic mycelium. Somebody please correct me if I'm wrong.

I hope this helps. You should probably check out TMC even if I did manage to make sense above.


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"The real voyage of discovery consists not in seeking new landscapes but in having new eyes."
- Marcel Proust

I wish you all ceaselessly flowing moments of happiness.

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Re: Isolation necessary for clones grown on agar? [Re: psyphon]
    #910201 -

That is something I wonder about also. According to Stamets Ps. cubensis is ideally supposed to display rhizomorphic mycelia, but my zebra has some agar plates of an old Hawk's Eye B+ print which have pins forming on them with entirely cottony mycelium. ?? Not a rhizo in sight yet.


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Re: Isolation necessary for clones grown on agar? [Re: Alkaloids]
    #913349 -

Because Cubensis fruits easily - even cottony mycelia will fruit. It takes a while to create an isolate that is purely rhizomorphic and is not sectoring - normally sectoring ALWAYS occurs with even the most rhizomorphic isolates because the nature of mushroom mycelia is that it is alwas changing. Ive seen highly rhizomorphic isolates produce nothing but cotton when isolated


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Re: Isolation necessary for clones grown on agar? [Re: Green_Velvet]
    #919015 -

Thanks for all the replies guys. It seems that there isn't a defenative anwser to my question. So I'm doing a little experminetation to try and find out for myself. I'll let everyone know how it goes.

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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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