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Offlinevatoloco
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Post deleted by MOE THE MAD SCIENTIST
    #552506 - 02/15/02 01:57 PM (15 years, 5 months ago)



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InvisibleOlgualion
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Re: cloning without agar? [Re: vatoloco]
    #552545 - 02/15/02 02:28 PM (15 years, 5 months ago)

One way is:
You could do a tissue sample to liquid medium (ie.honeywater, dextrose water, light malt water).

Or you could make a mycelial slurry with the fruitbody.

I really doubt that the tissue sample to sterilised rye would work though.


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Offlinekingkc
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Re: cloning without agar? [Re: Olgualion]
    #552584 - 02/15/02 03:00 PM (15 years, 5 months ago)

I would cook some white rice so it is slightly overdone.
I would then put about a quarter cup cooked white rice in a pint widemouth.
After sterilizing for the customary time I'd let that jar cool .
Now, very carefully take a sample of tissue and transfer that to the jar of cooked ,sterilized white rice.
Thats the best way to proceed if you lack agar.
Peace.


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InvisibleCLuB99
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Re: cloning without agar? [Re: vatoloco]
    #552739 - 02/15/02 05:17 PM (15 years, 5 months ago)

you can either use honey water or a mycelium slurry....
both works fine....with a personal preference to honey water...
or you can use peroxidated agar....


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Offlinecookiewhore
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Re: cloning without agar? [Re: CLuB99]
    #561754 - 02/24/02 07:25 PM (15 years, 4 months ago)

Whats your honeywater recipe? i have yet to try this
i tried, and fauiled myserably, with ME Liquid.

What about the slurry? how do you keep that sterile? whats your recipe with this?


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InvisibleZen Peddler
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Re: cloning without agar? [Re: cookiewhore]
    #561832 - 02/24/02 08:43 PM (15 years, 4 months ago)

Lizardking is the man to speak to about this... From my personal experiences you'll need to use h202 as liberally as possible.


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InvisibleCLuB99
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Re: cloning without agar? [Re: cookiewhore]
    #562264 - 02/25/02 08:50 AM (15 years, 4 months ago)

you can find the honey tek here: http://www.shroomery.org/findorgrowthem.php?View=docs&doc=112
you can prepare the slurry blending a clean piece of mushroom in sterile water and adding a couple cc's of H2O2 every 100cc of water.


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InvisibleZen Peddler
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Re: cloning without agar? [Re: CLuB99]
    #562872 - 02/25/02 07:46 PM (15 years, 4 months ago)

Yeah i used a piece of mushroom tissue that was coffee grinded into a boil of h202. I then used a govebox to transfer it into malt/water and brf paste that was - as Club99 suggests about 2mls of h202 to every 100mls. It worked great!!


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