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 Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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 Corporal Kielbasa
Registered: 05/29/04
Posts: 17,235
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Check out the local library or go to borders and sit there with a note pad if you cant afford the book just read it and leave it.
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 KaptKid
Spaced Pirate
 
Registered: 12/11/03
Posts: 6,252
Loc: Bright Side of the Sun
Last seen: 3 years, 1 month
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 Re: Creating an isolate  [Re: EonTan]
#3992268 - 03/30/05 08:03 PM (17 years, 11 months ago) |
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Sorry for the neb?
The clone has gone from agar to bulk and has fruited. Does the spores of this isolate, represent this isolate or does it take it back to "in general"???
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Child of the 60's, Tripping ever since.
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debianlinux
Myconerd - DBK
Registered: 12/09/02
Posts: 8,334
Loc: Over There
Last seen: 5 months, 27 days
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Re: Creating an isolate [Re: KaptKid]
#3992283 - 03/30/05 08:07 PM (17 years, 11 months ago) |
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Quote:
KaptKid said:
The clone has gone from agar to bulk and has fruited. Does the spores of this isolate, represent this isolate or does it take it back to "in general"???
the latter. a section of fruitbody mycelia would represent a modified/evolved version of the isolate. only another piece of the original isolate would truly represent the original isolate. the spores are definitely more in the direction of "in general".
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KaptKid
Spaced Pirate
Registered: 12/11/03
Posts: 6,252
Loc: Bright Side of the Sun
Last seen: 3 years, 1 month
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Re: Creating an isolate [Re: debianlinux]
#3992295 - 03/30/05 08:10 PM (17 years, 11 months ago) |
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Thanks
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Child of the 60's, Tripping ever since.
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NecroMyce
Mostly Ghostly
Registered: 05/12/13
Posts: 747
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Re: Creating an isolate [Re: EonTan]
#18385536 - 06/07/13 10:14 PM (9 years, 9 months ago) |
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Quote:
EonTan said:
Spore syringe>jar of substrate>casing>fruit. You then clone a piece of this fruit onto agar. When it grows out on agar, you transfer pieces to as many plates of AGAR as you think you might need to use now, and would like to store for the future. This is the easiest way. Every plate will have the same non sectoring substrain, becasue you cloned a fruit.
you have several to test for a good one.
I understand this method and why it is used. but just a quick question i have yet to find in servery posts ive been searching through. You say you have several to test for a good one, if i did the said method above of cloning/isolating... when i pick the fruits, i dont necessarily know if they're potent or not, and if they're worth it or out. so id obviously have to eat them and try them out.
Would i just have to pick a few fruits and cut a piece of the fruit off and drop it in agar to start colonizing it, and then try the fruits separately and decide which fruits i liked better? then from there i can transfer the agar and get a master culture?
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wildernessjunkie
Reshitivest
Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
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Re: Creating an isolate [Re: NecroMyce]
#18386549 - 06/08/13 05:09 AM (9 years, 9 months ago) |
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Dammit! I read all the way to the end of this before I realized it was an 8 year old thread.
The best resource for you right here is this link.
www.mushroomvideos.com
Spend the 9-10 bucks to download it. Its worth it, and will answer a whole slew of questions you're bound to have.
Edited by wildernessjunkie (06/08/13 05:10 AM)
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PanzerCubed
Registered: 11/22/12
Posts: 2,285
Loc: Nauru 
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Good thread but a tad old, thought this might be relevant.
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NecroMyce
Mostly Ghostly
Registered: 05/12/13
Posts: 747
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Quote:
wildernessjunkie said:
Dammit! I read all the way to the end of this before I realized it was an 8 year old thread.
The best resource for you right here is this link.
www.mushroomvideos.com
Spend the 9-10 bucks to download it. Its worth it, and will answer a whole slew of questions you're bound to have.
those videos are a waste, unless theres like 3 sections of videos to buy? cuz i bought it for 8.99$ and it only came with PF tek, Spore prints/syringes and a few other videos, didnt cover half the things i needed it to cover.
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clueless
justnew
Registered: 07/21/03
Posts: 774
Loc: ANE 
Last seen: 49 minutes, 12 seconds
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Re: Creating an isolate [Re: NecroMyce]
#18388411 - 06/08/13 04:03 PM (9 years, 9 months ago) |
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I have the 3rd edition on dvd.
I didn't download it so I'm not sure what you might be missing.
Disk 1 has
Brown rice flour
Grain Prep
Grain to Grain
Rye Grass seed
Manure Sub
Casing Prep
Straw Pasteurization
Laundry Basket- Straw Log
Disk 2 has
Agar and Petri dish work
Isolation of strains
Cloning
Culture Slants
Agar Inoculation
Sawdust-Woodchips
Inoculating Sawdust
Spore printing and Syringes
Not sure what else you need.
This seems like a good start point to me.
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I'm a rhinestone tiger in a leisure suit.
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NecroMyce
Mostly Ghostly
Registered: 05/12/13
Posts: 747
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Re: Creating an isolate [Re: clueless]
#18388445 - 06/08/13 04:18 PM (9 years, 9 months ago) |
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Well then i only have disk 1, how come i didnt get disk two with the two dvd package i bought? wtf
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clueless
justnew
Registered: 07/21/03
Posts: 774
Loc: ANE
Last seen: 49 minutes, 12 seconds
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Re: Creating an isolate [Re: NecroMyce]
#18388454 - 06/08/13 04:21 PM (9 years, 9 months ago) |
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You said you downloaded it for $8.99.
Did you do that or buy a box that should have 2 disks?
I am confused now.
I took very detailed notes from disk 1 in my journal.
Feel free to read through it, and pm for help.
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I'm a rhinestone tiger in a leisure suit.
Edited by clueless (06/08/13 04:26 PM)
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NecroMyce
Mostly Ghostly
Registered: 05/12/13
Posts: 747
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Re: Creating an isolate [Re: clueless]
#18388466 - 06/08/13 04:24 PM (9 years, 9 months ago) |
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Let's Grow Mushrooms Download, High Resolution $8.99!
You can now download the full release of Let's Grow Mushrooms in high resolution in two popular formats. The FLV version uses Flash video and plays great using Adobe Flash, Real Player, Quicktime, and many other players available free online. The MP4 version plays well on computers, but is also the standard for portable devices such as iPads, Androids, etc. Download both for less than ten dollars!
----------------------------------
Thats the one i downloaded... Or is it the 49$ one that has both disks?
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clueless
justnew
Registered: 07/21/03
Posts: 774
Loc: ANE
Last seen: 49 minutes, 12 seconds
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Re: Creating an isolate [Re: NecroMyce]
#18388510 - 06/08/13 04:38 PM (9 years, 9 months ago) |
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The sample shows all the same chapters, you should have the same in your download.
Contact them if you didn't get all chapters.
ps I have 3rd but the 4th edition appears to have more on it.
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I'm a rhinestone tiger in a leisure suit.
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NecroMyce
Mostly Ghostly
Registered: 05/12/13
Posts: 747
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Re: Creating an isolate [Re: clueless]
#18390691 - 06/09/13 12:31 AM (9 years, 9 months ago) |
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sure nuff he hooked it up 
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